Detailed information    

experimental Experimentally validated

Overview

Name   pilB   Type   Machinery gene
Locus tag   HI_0298 Genome accession   L42023
Coordinates   332256..333650 (-) Length   464 a.a.
NCBI ID   AAC21962.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

All genes in the comABCDE, comNOPQ, and pilABCD operons play essential roles in competence.


Genomic Context

Location: 327256..338650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0291 - 327506..327712 (-) 207 AAC21964.1 predicted coding region HI0291 -
  HI_0292 - 327787..327993 (-) 207 AAC21956.1 mercuric ion scavenger protein (merP) -
  HI_0293 - 328070..328456 (+) 387 AAC21957.1 transcriptional regulator, merR family, putative -
  HI_0294 - 328470..328787 (-) 318 AAC21958.1 met repressor (metJ) -
  HI_0295 - 329034..330296 (+) 1263 AAC21959.1 transcription termination factor (rho) -
  HI_0296 pilD 330350..331042 (-) 693 AAC21960.1 type 4 prepilin-like protein specific leader peptidase (hopD) Machinery gene
  HI_0297 pilC 331039..332259 (-) 1221 AAC21961.1 protein transport protein Machinery gene
  HI_0298 pilB 332256..333650 (-) 1395 AAC21962.1 protein transport protein Machinery gene
  HI_0299 pilA 333647..334096 (-) 450 AAC21963.1 prepilin peptidase dependent protein D Machinery gene
  HI_0300 - 334212..334766 (+) 555 AAC21965.1 ampD signalling protein (ampD) -
  HI_0301 - 335399..336298 (+) 900 AAC21966.1 hemolysin, putative -
  HI_0302 - 336282..337850 (+) 1569 AAC21967.1 apolipoprotein N-acyltransferase (cutE) -
  HI_0303 - 337900..338637 (+) 738 AAC21968.1 conserved hypothetical protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52983.51 Da        Isoelectric Point: 6.0235

>NTDB_id=1355 HI_0298 AAC21962.1 332256..333650(-) (pilB) [Haemophilus influenzae Rd KW20]
MTSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPNQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRVLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFNRLLR
TFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIKNSLLLVIAQRLVRKLCSKC
GGNLANSCDCHQGYRGRIGVYQFLHWQQNDYQTDFKNLRASGLEKVSQGITDEKEIERVLGKNL

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1355 HI_0298 AAC21962.1 332256..333650(-) (pilB) [Haemophilus influenzae Rd KW20]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTCTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGAACCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAATTTCAAGTGCGGTTTAGAATTGATGGAGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAGAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACGTGTGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAATGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAACCGCCTATTACGC
ACTTTTTTACGTCAAGATCCCGATATTATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCATTACGTGC
GGCACAAACTGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCTATTTCTCGCTTACAACAACTCG
GTATTCAACAGTATGAAATTAAAAACAGTTTATTGCTCGTTATTGCACAGCGTCTTGTACGAAAGCTTTGTTCAAAGTGC
GGTGGAAATTTAGCAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGACTATCAAACGGATTTTAAAAATTTACGTGCGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACGGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAATTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-98)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.544

99.784

0.963
  pilB Glaesserella parasuis strain SC1401

58.093

97.831

0.568
  pilB Vibrio cholerae strain A1552

39.279

100

0.446
  pilB Vibrio campbellii strain DS40M4

40.412

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Acinetobacter baylyi ADP1

39.113

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.414
  pilB Acinetobacter baumannii D1279779

39.709

100

0.412
  pilF Neisseria gonorrhoeae MS11

36.765

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Thermus thermophilus HB27

36.134

100

0.371

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] Brian A Dougherty et al. (1999) Identification of Haemophilus influenzae Rd transformation genes using cassette mutagenesis. Microbiology (Reading, England) 145 ( Pt 2):401-409. [PMID: 10075422]