Detailed information    

experimental Experimentally validated

Overview

Name   pilD   Type   Machinery gene
Locus tag   HI_0296 Genome accession   L42023
Coordinates   330350..331042 (-) Length   230 a.a.
NCBI ID   AAC21960.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

All genes in the comABCDE, comNOPQ, and pilABCD operons play essential roles in competence.


Genomic Context

Location: 325350..336042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0290 - 325363..327531 (-) 2169 AAC21955.1 potassium/copper-transporting ATPase, putative -
  HI_0291 - 327506..327712 (-) 207 AAC21964.1 predicted coding region HI0291 -
  HI_0292 - 327787..327993 (-) 207 AAC21956.1 mercuric ion scavenger protein (merP) -
  HI_0293 - 328070..328456 (+) 387 AAC21957.1 transcriptional regulator, merR family, putative -
  HI_0294 - 328470..328787 (-) 318 AAC21958.1 met repressor (metJ) -
  HI_0295 - 329034..330296 (+) 1263 AAC21959.1 transcription termination factor (rho) -
  HI_0296 pilD 330350..331042 (-) 693 AAC21960.1 type 4 prepilin-like protein specific leader peptidase (hopD) Machinery gene
  HI_0297 pilC 331039..332259 (-) 1221 AAC21961.1 protein transport protein Machinery gene
  HI_0298 pilB 332256..333650 (-) 1395 AAC21962.1 protein transport protein Machinery gene
  HI_0299 pilA 333647..334096 (-) 450 AAC21963.1 prepilin peptidase dependent protein D Machinery gene
  HI_0300 - 334212..334766 (+) 555 AAC21965.1 ampD signalling protein (ampD) -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26788.82 Da        Isoelectric Point: 9.2892

>NTDB_id=1357 HI_0296 AAC21960.1 330350..331042(-) (pilD) [Haemophilus influenzae Rd KW20]
MIYFTMFLLGGILGIALWFYLSGFITRLQQNIYAIYVELFPQNRSPFQPHFASIQQKKCGHILRYFFSIGVGFIFLQIAF
KDSIFTVWIGLTLIILWTISYLDWHYQLISTTPCLWLLTLGLFGADNNFSLLTLSESIKSAASFFIVFYVIYWLAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHRKKKEFLPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=1357 HI_0296 AAC21960.1 330350..331042(-) (pilD) [Haemophilus influenzae Rd KW20]
ATGATTTATTTCACAATGTTTTTATTAGGCGGTATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACGCG
TTTGCAGCAAAATATTTATGCGATTTACGTTGAATTATTTCCACAAAACCGTTCTCCATTTCAACCGCACTTTGCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTTAGTATTGGGGTTGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGATCGGACTCACACTTATTATTCTTTGGACAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACGACACCCTGTTTATGGTTACTTACTCTAGGATTATTTGGAGCAGACAATAACTTTTCATTACTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGTAATCTATTGGCTTGCAAAATTCTATTATGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTGCTTGGAATATGTTTTTCGCTTATTCATAGGAAGAAAAAAGAATTTTTACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

94.783

100

0.948

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] Brian A Dougherty et al. (1999) Identification of Haemophilus influenzae Rd transformation genes using cassette mutagenesis. Microbiology (Reading, England) 145 ( Pt 2):401-409. [PMID: 10075422]