Detailed information    

experimental Experimentally validated

Overview

Name   pilA   Type   Machinery gene
Locus tag   HI_0299 Genome accession   L42023
Coordinates   333647..334096 (-) Length   149 a.a.
NCBI ID   AAC21963.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

All genes in the comABCDE, comNOPQ, and pilABCD operons play essential roles in competence.


Genomic Context

Location: 328647..339096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0295 - 329034..330296 (+) 1263 AAC21959.1 transcription termination factor (rho) -
  HI_0296 pilD 330350..331042 (-) 693 AAC21960.1 type 4 prepilin-like protein specific leader peptidase (hopD) Machinery gene
  HI_0297 pilC 331039..332259 (-) 1221 AAC21961.1 protein transport protein Machinery gene
  HI_0298 pilB 332256..333650 (-) 1395 AAC21962.1 protein transport protein Machinery gene
  HI_0299 pilA 333647..334096 (-) 450 AAC21963.1 prepilin peptidase dependent protein D Machinery gene
  HI_0300 - 334212..334766 (+) 555 AAC21965.1 ampD signalling protein (ampD) -
  HI_0301 - 335399..336298 (+) 900 AAC21966.1 hemolysin, putative -
  HI_0302 - 336282..337850 (+) 1569 AAC21967.1 apolipoprotein N-acyltransferase (cutE) -
  HI_0303 - 337900..338637 (+) 738 AAC21968.1 conserved hypothetical protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15509.02 Da        Isoelectric Point: 9.1085

>NTDB_id=1354 HI_0299 AAC21963.1 333647..334096(-) (pilA) [Haemophilus influenzae Rd KW20]
MKLTTLQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTSCTGGKNGIA
ADIKTAKGYVASVITQSGGITVKGNGTLANMEYILQAKGNAAAGVTWTTTCKGTDASLFPANFCGSVTK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1354 HI_0299 AAC21963.1 333647..334096(-) (pilA) [Haemophilus influenzae Rd KW20]
ATGAAATTAACAACACTGCAAACCTTGAAAAAAGGGTTTACATTAATCGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACCATCGCAATTCCTTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCGTCTGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAGCTGTACAGGGGGAAAAAATGGTATTGCA
GCGGATATAAAGACAGCAAAAGGCTATGTAGCCTCAGTTATCACTCAATCAGGTGGTATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTAAAGGTAATGCTGCAGCAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

91.946

100

0.919
  pilA Glaesserella parasuis strain SC1401

55.034

100

0.55
  pilA2 Legionella pneumophila str. Paris

44.094

93.382

0.412
  pilA2 Legionella pneumophila strain ERS1305867

43.307

93.382

0.404
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] Brian A Dougherty et al. (1999) Identification of Haemophilus influenzae Rd transformation genes using cassette mutagenesis. Microbiology (Reading, England) 145 ( Pt 2):401-409. [PMID: 10075422]