Detailed information    

experimental Experimentally validated

Overview

Name   pilC   Type   Machinery gene
Locus tag   HI_0297 Genome accession   L42023
Coordinates   331039..332259 (-) Length   406 a.a.
NCBI ID   AAC21961.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

All genes in the comABCDE, comNOPQ, and pilABCD operons play essential roles in competence.


Genomic Context

Location: 326039..337259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0291 - 327506..327712 (-) 207 AAC21964.1 predicted coding region HI0291 -
  HI_0292 - 327787..327993 (-) 207 AAC21956.1 mercuric ion scavenger protein (merP) -
  HI_0293 - 328070..328456 (+) 387 AAC21957.1 transcriptional regulator, merR family, putative -
  HI_0294 - 328470..328787 (-) 318 AAC21958.1 met repressor (metJ) -
  HI_0295 - 329034..330296 (+) 1263 AAC21959.1 transcription termination factor (rho) -
  HI_0296 pilD 330350..331042 (-) 693 AAC21960.1 type 4 prepilin-like protein specific leader peptidase (hopD) Machinery gene
  HI_0297 pilC 331039..332259 (-) 1221 AAC21961.1 protein transport protein Machinery gene
  HI_0298 pilB 332256..333650 (-) 1395 AAC21962.1 protein transport protein Machinery gene
  HI_0299 pilA 333647..334096 (-) 450 AAC21963.1 prepilin peptidase dependent protein D Machinery gene
  HI_0300 - 334212..334766 (+) 555 AAC21965.1 ampD signalling protein (ampD) -
  HI_0301 - 335399..336298 (+) 900 AAC21966.1 hemolysin, putative -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 46290.54 Da        Isoelectric Point: 9.8695

>NTDB_id=1356 HI_0297 AAC21961.1 331039..332259(-) (pilC) [Haemophilus influenzae Rd KW20]
MTKKLFYYQASNPLNQKQKGSIIADTKQQAHFQLISRGLTHIKLQQNWQFGAKPKNSEISELLNQLATLLQSAIPLKNSL
QILQQNCTQIVLNEWLERLLQSIESGLAFSQAIEQQGKYLTQQEIQLIQVGEMTGKLAVVCKKIATHRSQSLALQRKLQK
IMLYPSMVLGISLLLTLALLLFIVPQFAEMYSGNNAELPTITAILLSISNFLKQNIGILLFFVLSFFLFYYFYLKRQTWF
YQKKNQLISITPIFGTIQKLSRLVNFSQSLQIMLQAGVPLNQALDSFLPRTQTWQTKKTLVNDIVLDKEVRSILQWVSQG
YAFSNSVSSDLFPMEAQQMLQIGEQSGKLALMLEHIAENYQEKLNHQIDLLSQMLEPLMMVIIGSLIGIIMMGMYLPIFN
MGSVIQ

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1356 HI_0297 AAC21961.1 331039..332259(-) (pilC) [Haemophilus influenzae Rd KW20]
ATGACTAAAAAACTCTTTTATTATCAAGCTAGTAACCCATTAAATCAGAAACAAAAAGGCTCAATTATTGCGGATACAAA
ACAACAAGCACACTTTCAATTAATAAGCCGCGGGCTTACTCACATCAAATTACAACAAAACTGGCAATTTGGGGCAAAGC
CCAAAAATTCAGAAATTAGCGAATTACTCAATCAATTAGCCACGTTGCTACAATCCGCTATTCCGTTAAAAAACAGTCTG
CAAATTTTGCAACAAAATTGTACTCAAATTGTACTCAATGAATGGCTTGAACGACTACTTCAATCTATTGAATCTGGTTT
AGCATTCTCACAAGCCATTGAACAACAAGGGAAATATCTCACACAACAAGAAATTCAACTGATTCAAGTGGGAGAAATGA
CGGGAAAACTTGCCGTAGTTTGTAAAAAAATAGCCACACATCGCAGCCAATCTTTAGCATTACAACGTAAATTACAGAAA
ATTATGTTATATCCCTCAATGGTATTGGGAATTTCTCTATTATTGACACTCGCATTACTGCTTTTTATCGTGCCTCAATT
TGCTGAAATGTACAGTGGCAACAATGCGGAGTTACCAACAATAACCGCAATATTGCTCTCAATATCTAATTTCCTTAAGC
AAAATATTGGCATTTTGCTATTTTTCGTTTTGAGTTTTTTTCTATTTTATTATTTCTATCTAAAACGCCAGACTTGGTTT
TATCAAAAGAAAAATCAACTTATTTCTATCACGCCTATTTTTGGCACAATTCAAAAGCTTTCACGTTTAGTGAACTTTAG
TCAAAGTTTACAAATTATGTTGCAAGCTGGCGTACCGCTTAATCAGGCACTAGACAGTTTTCTCCCTCGCACACAAACGT
GGCAAACCAAGAAAACGCTTGTAAATGACATCGTATTAGATAAAGAAGTGCGGTCAATTTTGCAATGGGTTTCTCAAGGC
TATGCGTTTTCTAATAGTGTAAGTAGCGATCTTTTCCCGATGGAAGCACAACAAATGCTACAAATTGGCGAGCAAAGCGG
AAAACTCGCTTTGATGCTAGAGCATATTGCTGAAAATTACCAAGAAAAACTTAATCATCAAATTGACTTACTCTCACAAA
TGCTAGAACCATTAATGATGGTGATCATCGGCAGTCTGATTGGAATTATTATGATGGGAATGTATTTACCTATCTTTAAT
ATGGGTTCTGTTATTCAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(266-397)

Type II secretion system (T2SS), protein F (T2SSF)

(63-185)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Haemophilus influenzae 86-028NP

97.783

100

0.978
  pilC Glaesserella parasuis strain SC1401

38.155

100

0.384

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] Brian A Dougherty et al. (1999) Identification of Haemophilus influenzae Rd transformation genes using cassette mutagenesis. Microbiology (Reading, England) 145 ( Pt 2):401-409. [PMID: 10075422]