Detailed information    

experimental Experimentally validated

Overview

Name   pilC   Type   Machinery gene
Locus tag   NTHI_RS01945 Genome accession   NC_007146
Coordinates   393807..395027 (-) Length   406 a.a.
NCBI ID   WP_011271971.1    Uniprot ID   Q4QNP6
Organism   Haemophilus influenzae 86-028NP     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

The pilABCD and comABCDEF gene clusters are operons and that the product of each gene is essential for normal pilus function. Mutants with nonpolar deletions in each of the 10 pil and com genes had an adherence defect when primary human airway cells were used as the target. These mutants were also diminished in their ability to form a biofilm in vitro and, additionally, were deficient in natural transformation.


Genomic Context

Location: 388807..400027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHI_RS01910 - 389976..390182 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  NTHI_RS01915 - 390267..390473 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  NTHI_RS01920 - 390558..390764 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  NTHI_RS01925 (NTHI0403) - 390841..391227 (+) 387 WP_005631185.1 Cu(I)-responsive transcriptional regulator -
  NTHI_RS01930 (NTHI0404) metJ 391241..391558 (-) 318 WP_005634287.1 met regulon transcriptional regulator MetJ -
  NTHI_RS01935 (NTHI0405) rho 391802..393064 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  NTHI_RS01940 (NTHI0406) pilD 393118..393810 (-) 693 WP_011271970.1 prepilin peptidase Machinery gene
  NTHI_RS01945 (NTHI0407) pilC 393807..395027 (-) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  NTHI_RS01950 (NTHI0408) pilB 395024..396418 (-) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  NTHI_RS01955 (NTHI0409) pilA 396415..396864 (-) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NTHI_RS01960 (NTHI0411) ampD 396979..397539 (+) 561 WP_011271974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NTHI_RS01965 (NTHI0412) corC 398161..399060 (+) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 46259.57 Da        Isoelectric Point: 10.0444

>NTDB_id=1367 NTHI_RS01945 WP_011271971.1 393807..395027(-) (pilC) [Haemophilus influenzae 86-028NP]
MTKKLFYYQGSNALNQKQKGSIIADTKQQAHFQLISRGLTHIKLQQNWQFGAKPKNSEISELLNQLATLLQSVIPLKNSL
QILQQNCTQIMLNKWLERLLQSIESGLAFSQAIEQQGKYLTQQEIQLIQVGEMTGKLAVVCKKIATHRSQSLALQRKLQK
IMLYPSMVLGISLLLTLALLLFIAPQFAEMYSGNNAELPTITAILLSISNFLKQNIGILLFFVLSFFLFYYFYLKRQTWF
HQKKNQLISITPIFGTIQKLSRLVNFSQSLQIMLQAGVPLNQALDSFLPRTQTWQTKKTLVNDMVLDKEVRSILQWVSQG
YAFSNSVSSDLFPMEAQQMLQIGEQSGKLALMLEHIADNYQEKLNHQIDLLSQMLEPLMMVIIGSLIGIIMMGMYLPIFN
MGSVIQ

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1367 NTHI_RS01945 WP_011271971.1 393807..395027(-) (pilC) [Haemophilus influenzae 86-028NP]
ATGACTAAAAAACTCTTTTATTATCAAGGTAGTAACGCATTAAATCAGAAACAAAAAGGCTCAATTATTGCGGATACGAA
ACAACAAGCGCACTTTCAGTTAATAAGCCGCGGGCTTACTCACATCAAATTACAACAAAACTGGCAATTTGGGGCAAAAC
CCAAAAATTCAGAAATCAGTGAATTACTCAATCAATTAGCGACATTGCTACAGTCCGTAATTCCGTTAAAAAACAGCCTA
CAAATTTTGCAACAAAATTGTACTCAAATTATGCTCAACAAATGGCTTGAACGACTGCTTCAATCCATTGAATCTGGCTT
AGCATTCTCACAAGCCATTGAACAACAAGGAAAATATCTCACACAACAAGAAATTCAACTGATTCAAGTGGGAGAAATGA
CAGGAAAACTTGCCGTAGTTTGTAAAAAAATAGCCACGCACCGTAGTCAATCTTTGGCTTTACAACGCAAATTACAGAAA
ATTATGTTATATCCCTCAATGGTATTGGGAATTTCTCTATTATTGACACTCGCATTACTGCTTTTTATCGCGCCTCAATT
TGCTGAAATGTACAGTGGCAATAATGCGGAGTTACCAACAATAACCGCAATATTGCTCTCAATATCTAATTTCCTTAAGC
AAAATATTGGCATTTTGCTATTTTTCGTTTTGAGTTTTTTTCTATTTTATTATTTCTATCTAAAACGCCAGACTTGGTTT
CATCAAAAGAAAAATCAACTTATTTCTATCACGCCTATTTTTGGCACAATTCAAAAGCTTTCACGTTTAGTGAACTTTAG
TCAAAGTTTACAAATTATGTTGCAGGCCGGCGTACCGCTTAATCAGGCACTAGACAGTTTTCTTCCTCGCACACAAACTT
GGCAAACCAAGAAAACGCTTGTAAACGATATGGTATTAGATAAAGAAGTGCGGTCAATTTTGCAATGGGTTTCTCAAGGC
TATGCGTTTTCTAATAGCGTAAGTAGCGATCTTTTCCCGATGGAAGCACAACAAATGCTACAAATTGGCGAACAAAGCGG
AAAACTCGCTTTGATGCTAGAGCATATCGCAGATAATTACCAAGAAAAACTTAATCATCAAATTGACTTACTCTCACAAA
TGCTAGAACCATTAATGATGGTAATCATCGGCAGTCTGATTGGGATTATTATGATGGGAATGTATTTACCTATCTTTAAT
ATGGGATCAGTTATTCAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(63-185)

Type II secretion system (T2SS), protein F (T2SSF)

(266-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4QNP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Haemophilus influenzae Rd KW20

97.783

100

0.978
  pilC Glaesserella parasuis strain SC1401

38.155

100

0.384

Multiple sequence alignment    



References

[1] Michael D Carruthers et al. (2012) Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons. Journal of Bacteriology 194(8):1927-33. [PMID: 22328674]