Detailed information NTG0001368

Experimentally validated

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NTHI_RS01940
• Genome accession: NC_007146
• Coordinates: 393118..393810 (-)
• Length: 230 a.a.
• NCBI ID: WP_011271970.1
• Uniprot ID: -
• Organism: Haemophilus influenzae 86-028NP
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

The pilABCD and comABCDEF gene clusters are operons and that the product of each gene is essential for normal pilus function. Mutants with nonpolar deletions in each of the 10 pil and com genes had an adherence defect when primary human airway cells were used as the target. These mutants were also diminished in their ability to form a biofilm in vitro and, additionally, were deficient in natural transformation.

Genomic Context

Location: 388118..398810

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NTHI_RS01910	-	389976..390182 (-)	207	WP_005631184.1	heavy-metal-associated domain-containing protein	-
NTHI_RS01915	-	390267..390473 (-)	207	WP_005631184.1	heavy-metal-associated domain-containing protein	-
NTHI_RS01920	-	390558..390764 (-)	207	WP_005631184.1	heavy-metal-associated domain-containing protein	-
NTHI_RS01925 (NTHI0403)	-	390841..391227 (+)	387	WP_005631185.1	Cu(I)-responsive transcriptional regulator	-
NTHI_RS01930 (NTHI0404)	metJ	391241..391558 (-)	318	WP_005634287.1	met regulon transcriptional regulator MetJ	-
NTHI_RS01935 (NTHI0405)	rho	391802..393064 (+)	1263	WP_005648966.1	transcription termination factor Rho	-
NTHI_RS01940 (NTHI0406)	pilD	393118..393810 (-)	693	WP_011271970.1	prepilin peptidase	Machinery gene
NTHI_RS01945 (NTHI0407)	pilC	393807..395027 (-)	1221	WP_011271971.1	type II secretion system F family protein	Machinery gene
NTHI_RS01950 (NTHI0408)	pilB	395024..396418 (-)	1395	WP_011271972.1	GspE/PulE family protein	Machinery gene
NTHI_RS01955 (NTHI0409)	pilA	396415..396864 (-)	450	WP_011271973.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
NTHI_RS01960 (NTHI0411)	ampD	396979..397539 (+)	561	WP_011271974.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-

Sequence

Protein

• Length: 230 a.a.
• Molecular weight: 26682.66 Da
• Isoelectric Point: 8.6876

>NTDB_id=1368 NTHI_RS01940 WP_011271970.1 393118..393810(-) (pilD) [Haemophilus influenzae 86-028NP]
MIYFTMFLLGGILGIALWFYLSGFITHLQQEIYATYVELFPQNSSPFQPHFASIQQKKCGHILRYFFSIGVGFIFLQIAF
KDSIFTVWIGLTLIILWTISYLDWHYQLISTTPCLWLLTLGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKCYYR
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAIIIYLVKYYGY

Nucleotide

• Length: 693 bp

>NTDB_id=1368 NTHI_RS01940 WP_011271970.1 393118..393810(-) (pilD) [Haemophilus influenzae 86-028NP]
ATGATTTACTTCACAATGTTTTTATTAGGCGGCATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACGCA
TTTGCAGCAAGAGATTTATGCGACTTACGTTGAATTATTTCCACAAAACAGTTCTCCATTTCAACCGCACTTTGCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTTAGTATTGGGGTTGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGATCGGACTCACACTTATTATTCTTTGGACAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACGACACCCTGTTTATGGTTACTTACTCTCGGTTTATTTGGCGCAGACAATAACTTTTCATTGCTAACGT
TATCTGAAAGCATAAAAAGTGCGGCTAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATGTTATTATAGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTGCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCGGCTATCATTATTTATCTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (92-199)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Haemophilus influenzae Rd KW20	94.783	100	0.948

References

• [1]: Michael D Carruthers et al. (2012) Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons. Journal of Bacteriology 194(8):1927-33. [PMID: 22328674]