Detailed information    

experimental Experimentally validated

Overview

Name   pilC   Type   Machinery gene
Locus tag   A4U84_RS08520 Genome accession   NZ_CP015099
Coordinates   1766573..1767769 (-) Length   398 a.a.
NCBI ID   WP_005711777.1    Uniprot ID   B8F826
Organism   Glaesserella parasuis strain SC1401     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

The expression levels of candidate uptake components were significantly lower than in SC1401, respectively. These results revealed that the highly naturally competent strain SC1401 up-regulated most of the candidate natural competence-related regulons/components.


Genomic Context

Location: 1761573..1772769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4U84_RS11985 (A4U84_08490) - 1761837..1762211 (+) 375 WP_075592870.1 SLC13 family permease -
  A4U84_RS08495 (A4U84_08495) - 1762289..1762678 (-) 390 WP_005711765.1 SufE family protein -
  A4U84_RS08500 (A4U84_08500) clpP 1762779..1763360 (+) 582 WP_010787003.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  A4U84_RS08505 (A4U84_08505) clpX 1763367..1764617 (+) 1251 WP_035491633.1 ATP-dependent protease ATP-binding subunit ClpX -
  A4U84_RS08510 (A4U84_08510) dapE 1764691..1765824 (-) 1134 WP_005711772.1 succinyl-diaminopimelate desuccinylase -
  A4U84_RS08515 (A4U84_08515) - 1765908..1766576 (-) 669 WP_005711775.1 prepilin peptidase -
  A4U84_RS08520 (A4U84_08520) pilC 1766573..1767769 (-) 1197 WP_005711777.1 type II secretion system F family protein Machinery gene
  A4U84_RS08525 (A4U84_08525) pilB 1767762..1769147 (-) 1386 WP_010787006.1 GspE/PulE family protein Machinery gene
  A4U84_RS08530 (A4U84_08530) pilA 1769195..1769659 (-) 465 WP_005711781.1 pilin Machinery gene
  A4U84_RS08535 (A4U84_08535) radA 1769847..1771223 (+) 1377 WP_021115532.1 DNA repair protein RadA -
  A4U84_RS08540 (A4U84_08540) gmk 1771276..1771902 (-) 627 WP_005711785.1 guanylate kinase -
  A4U84_RS08545 (A4U84_08545) gpt 1772140..1772619 (-) 480 WP_005714020.1 xanthine phosphoribosyltransferase -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45575.38 Da        Isoelectric Point: 10.1129

>NTDB_id=1385 A4U84_RS08520 WP_005711777.1 1766573..1767769(-) (pilC) [Glaesserella parasuis strain SC1401]
MNNIYEFQWKGRNRFGQKQTGRQLAESREVLEKRLQQKGYSQLSISRHFALPTAPKKEEINQFMQQLALLIQAKIPLKQS
LVMLLETCQNRTFYRWQQETIRLIEAGFALSVAFKKQGKYINPQEIQLIKMAETSGNLGLILTNIAQRREKSEKLAKKIK
KILFYPVFILAISITLSILLLLFIVPQFAELYGSKGKSLPLITEILFSLSHFLQHSILTLIILCVLCFLLIHILNKKTDL
IKRLKFIILSHIPILKGIIQYSRIIFFCQNSSLMLASHIRLDTVLHSFLANKSDDILLQGSLATTLTYLKQGYRLADSLD
PKLFPTDMLQMIAIGEQSGKLSPMLQQISDGYQQRLDYQIDILSQLLEPMLMVLMGIIVGTILVGLYLPIFDMGAMIE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=1385 A4U84_RS08520 WP_005711777.1 1766573..1767769(-) (pilC) [Glaesserella parasuis strain SC1401]
ATGAATAACATTTATGAATTTCAATGGAAAGGACGCAACCGCTTTGGGCAAAAGCAGACAGGGCGACAGCTTGCCGAAAG
CCGAGAAGTGCTAGAGAAACGGTTGCAACAAAAAGGATACAGCCAGTTAAGCATTAGCCGACATTTTGCCTTGCCGACTG
CACCAAAAAAGGAAGAAATTAATCAATTTATGCAACAGTTAGCCTTATTAATTCAAGCTAAAATCCCCCTAAAACAGAGT
TTAGTGATGTTGCTTGAAACCTGCCAAAATCGCACTTTTTACCGTTGGCAACAAGAAACAATCCGCTTGATTGAAGCCGG
CTTTGCGTTATCCGTTGCCTTTAAAAAACAGGGAAAATATATTAATCCACAAGAAATCCAATTAATTAAAATGGCAGAAA
CAAGCGGTAATTTAGGGCTAATTTTAACCAACATTGCACAACGCCGTGAAAAATCGGAAAAATTAGCCAAGAAAATTAAA
AAAATTCTCTTTTATCCCGTCTTTATTTTAGCTATTTCAATCACACTCTCTATTTTATTATTGCTTTTTATTGTGCCACA
ATTTGCAGAACTTTATGGTTCAAAAGGAAAATCACTCCCTCTAATTACCGAAATACTCTTTAGCCTATCCCATTTTCTAC
AACACTCTATTTTAACGCTAATTATTTTATGTGTGTTATGTTTTCTATTAATTCATATTTTAAATAAAAAAACAGATCTT
ATTAAACGCCTAAAGTTTATTATATTAAGCCATATTCCTATTTTAAAAGGCATTATCCAGTATTCACGCATTATCTTTTT
TTGTCAAAATAGCAGTTTAATGCTGGCTTCACATATTCGTTTAGATACTGTACTTCATTCCTTTTTAGCCAATAAAAGTG
ATGATATTTTATTACAAGGTTCTCTAGCAACTACACTAACTTATTTAAAACAAGGCTATCGTTTAGCCGACAGTTTAGAT
CCAAAACTCTTTCCTACGGATATGTTACAGATGATTGCCATTGGCGAGCAGAGCGGAAAATTATCGCCAATGTTGCAACA
AATCAGCGACGGTTATCAACAGCGGTTAGATTATCAAATTGATATACTTTCCCAATTATTAGAGCCGATGTTAATGGTGT
TAATGGGAATTATTGTAGGAACTATTTTAGTCGGGTTATATTTGCCGATTTTTGATATGGGAGCAATGATAGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(63-186)

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8F826

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Haemophilus influenzae 86-028NP

38.155

100

0.384
  pilC Haemophilus influenzae Rd KW20

38.155

100

0.384

Multiple sequence alignment    



References

[1] Ke Dai et al. (2018) Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401. Frontiers in Cellular And Infection Microbiology 8:32. [PMID: 29473023]