Detailed information    

experimental Experimentally validated

Overview

Name   pilA   Type   Machinery gene
Locus tag   A4U84_RS08530 Genome accession   NZ_CP015099
Coordinates   1769195..1769659 (-) Length   154 a.a.
NCBI ID   WP_005711781.1    Uniprot ID   K4JWE9
Organism   Glaesserella parasuis strain SC1401     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

The expression levels of candidate uptake components were significantly lower than in SC1401, respectively. These results revealed that the highly naturally competent strain SC1401 up-regulated most of the candidate natural competence-related regulons/components.


Genomic Context

Location: 1764195..1774659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4U84_RS08510 (A4U84_08510) dapE 1764691..1765824 (-) 1134 WP_005711772.1 succinyl-diaminopimelate desuccinylase -
  A4U84_RS08515 (A4U84_08515) - 1765908..1766576 (-) 669 WP_005711775.1 prepilin peptidase -
  A4U84_RS08520 (A4U84_08520) pilC 1766573..1767769 (-) 1197 WP_005711777.1 type II secretion system F family protein Machinery gene
  A4U84_RS08525 (A4U84_08525) pilB 1767762..1769147 (-) 1386 WP_010787006.1 GspE/PulE family protein Machinery gene
  A4U84_RS08530 (A4U84_08530) pilA 1769195..1769659 (-) 465 WP_005711781.1 pilin Machinery gene
  A4U84_RS08535 (A4U84_08535) radA 1769847..1771223 (+) 1377 WP_021115532.1 DNA repair protein RadA -
  A4U84_RS08540 (A4U84_08540) gmk 1771276..1771902 (-) 627 WP_005711785.1 guanylate kinase -
  A4U84_RS08545 (A4U84_08545) gpt 1772140..1772619 (-) 480 WP_005714020.1 xanthine phosphoribosyltransferase -
  A4U84_RS08550 (A4U84_08550) - 1772799..1774253 (+) 1455 WP_062924312.1 aminoacyl-histidine dipeptidase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16379.04 Da        Isoelectric Point: 9.3428

>NTDB_id=1383 A4U84_RS08530 WP_005711781.1 1769195..1769659(-) (pilA) [Glaesserella parasuis strain SC1401]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNVGDKKISECSSGKN
GVRADKSDVPDAKYLKSISVASGVITVAGKGNIDGYGYTMTPKFANNNITWATSCQGADISLFPANFCGQKATP

Nucleotide


Download         Length: 465 bp        

>NTDB_id=1383 A4U84_RS08530 WP_005711781.1 1769195..1769659(-) (pilA) [Glaesserella parasuis strain SC1401]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTAATGATCGTGATCGCCATCATTGC
CATTTTAGCAACAATAGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCAGCTCTCTCTGAGTTATTAAGTGCTTCCG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACGTAGGGGATAAAAAAATCTCGGAATGTAGTTCAGGTAAAAAT
GGCGTTCGAGCAGATAAATCCGATGTCCCTGACGCAAAATATCTTAAATCTATTTCCGTCGCTTCGGGGGTAATCACCGT
GGCAGGTAAAGGCAATATTGATGGCTACGGCTACACAATGACCCCGAAATTTGCTAATAACAACATCACTTGGGCAACAA
GCTGTCAAGGGGCTGACATTAGTTTGTTCCCTGCGAATTTTTGTGGGCAGAAAGCTACACCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K4JWE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

55.034

100

0.55
  pilA Haemophilus influenzae 86-028NP

54.362

100

0.544
  pilA2 Legionella pneumophila strain ERS1305867

38.235

100

0.382
  pilA2 Legionella pneumophila str. Paris

37.5

100

0.375

Multiple sequence alignment    



References

[1] Ke Dai et al. (2018) Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401. Frontiers in Cellular And Infection Microbiology 8:32. [PMID: 29473023]