Detailed information    

experimental Experimentally validated

Overview

Name   qstR   Type   Regulator
Locus tag   VP_RS13295 Genome accession   NC_004603
Coordinates   2865626..2866270 (+) Length   214 a.a.
NCBI ID   WP_005480993.1    Uniprot ID   Q87LA2
Organism   Vibrio parahaemolyticus RIMD 2210633     
Function   promote expression of competence genes   
Competence regulation

Function

The ΔtfoX or ΔcytR mutant showed significant downregulation in pilA, pilB, comEA, comEC, and qstR.


Genomic Context

Location: 2860626..2871270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP_RS13280 (VP2707) - 2861110..2862555 (-) 1446 WP_005481017.1 MSHA biogenesis protein MshI -
  VP_RS13285 (VP2708) csrD 2862567..2864576 (-) 2010 WP_011106082.1 RNase E specificity factor CsrD -
  VP_RS13290 (VP2709) ssb 2864817..2865347 (-) 531 WP_005466625.1 single-stranded DNA-binding protein Machinery gene
  VP_RS13295 (VP2710) qstR 2865626..2866270 (+) 645 WP_005480993.1 LuxR C-terminal-related transcriptional regulator Regulator
  VP_RS13300 (VP2711) galU 2866528..2867400 (+) 873 WP_005466627.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  VP_RS13305 (VP2712) uvrA 2867538..2870360 (+) 2823 WP_005490537.1 excinuclease ABC subunit UvrA -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  tfoX qstR positive effect
  cytR qstR positive effect
  tfoX comEC positive effect
  cytR comEC positive effect
  tfoX pilB positive effect
  cytR pilB positive effect
  tfoX comEA positive effect
  cytR comEA positive effect
  tfoX pilA positive effect
  cytR pilA positive effect

Sequence

Protein


Download         Length: 214 a.a.        Molecular weight: 24386.20 Da        Isoelectric Point: 8.7760

>NTDB_id=1296 VP_RS13295 WP_005480993.1 2865626..2866270(+) (qstR) [Vibrio parahaemolyticus RIMD 2210633]
MRKSAYARKLFLISMEDDAAQKVASLEKYIDMSIPVISTDALMEAKPEHRNKILLIDFSEHKSLVQSIKNLPLVWKNFET
VVFNVPKRLTTDELLAFGQLKGLFYSEDSLEQVGEGLKGIVNGQNWLPRNVTSQLLHYYRNVINTHTAPATVDLTIRELQ
VLRCLQAGASNSQMAEELFVSEFTIKSHLYQIFKKLSVKNRVQAIAWADQNLMS

Nucleotide


Download         Length: 645 bp        

>NTDB_id=1296 VP_RS13295 WP_005480993.1 2865626..2866270(+) (qstR) [Vibrio parahaemolyticus RIMD 2210633]
GTGAGAAAGTCGGCTTACGCAAGAAAGCTATTTCTGATTAGTATGGAAGACGATGCTGCGCAAAAAGTCGCATCGTTAGA
AAAGTACATCGATATGAGCATTCCTGTAATCTCAACCGATGCCCTAATGGAAGCGAAACCCGAACATCGAAATAAAATTC
TACTCATTGACTTCAGTGAACATAAATCACTCGTTCAATCGATTAAAAACCTGCCATTGGTTTGGAAGAACTTTGAGACG
GTCGTGTTCAACGTTCCAAAGCGCCTCACTACGGATGAACTGCTCGCCTTCGGTCAATTAAAAGGCTTGTTTTACAGCGA
AGATTCTTTAGAACAAGTCGGCGAAGGACTGAAAGGTATTGTCAACGGGCAAAACTGGCTACCACGCAACGTAACCAGCC
AACTGCTGCACTACTATCGAAATGTGATAAATACTCACACCGCGCCAGCAACCGTCGATCTCACCATTCGCGAACTGCAA
GTGCTGCGTTGTCTGCAAGCCGGGGCATCGAATAGCCAGATGGCAGAAGAACTGTTTGTTAGCGAATTTACGATCAAGTC
GCACCTGTATCAGATCTTTAAAAAGCTCTCAGTGAAAAACCGAGTTCAAGCCATTGCTTGGGCCGATCAAAACTTGATGT
CTTAA

Domains

Predicted by InterproScan.

Bacterial regulatory proteins, luxR family (GerE)

(154-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LA2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  qstR Vibrio campbellii strain DS40M4

89.252

100

0.893
  qstR Vibrio cholerae strain A1552

52.093

100

0.523

Multiple sequence alignment    



References

[1] Anusuya Debnath et al. (2022) Regulators of natural competence in Vibrio parahaemolyticus. Brazilian Journal of Microbiology : [publication of The Brazilian Society For Microbiology] 53(3):1491-1499. [PMID: 35761009]