Detailed information    

experimental Experimentally validated

Overview

Name   qstR   Type   Regulator
Locus tag   DSB67_RS13765 Genome accession   NZ_CP030788
Coordinates   2843948..2844592 (+) Length   214 a.a.
NCBI ID   WP_005432827.1    Uniprot ID   -
Organism   Vibrio campbellii strain DS40M4     
Function   promote expression of competence genes   
Competence regulation

Function

Endogenous qstR and ectopic tfoX expression are both necessary to initiate high levels of natural transformation in V. campbellii DS40M4.


Genomic Context

Location: 2838948..2849592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSB67_RS13750 (DSB67_13755) - 2839398..2840846 (-) 1449 WP_010648155.1 MSHA biogenesis protein MshI -
  DSB67_RS13755 (DSB67_13760) csrD 2840858..2842867 (-) 2010 WP_010648157.1 RNase E specificity factor CsrD -
  DSB67_RS13760 (DSB67_13765) ssb 2843115..2843654 (-) 540 WP_010648159.1 single-stranded DNA-binding protein Machinery gene
  DSB67_RS13765 (DSB67_13770) qstR 2843948..2844592 (+) 645 WP_005432827.1 LuxR C-terminal-related transcriptional regulator Regulator
  DSB67_RS13770 (DSB67_13775) galU 2844756..2845628 (+) 873 WP_010648161.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  DSB67_RS13775 (DSB67_13780) uvrA 2845767..2848589 (+) 2823 WP_010648164.1 excinuclease ABC subunit UvrA -

Sequence

Protein


Download         Length: 214 a.a.        Molecular weight: 24551.46 Da        Isoelectric Point: 8.0591

>NTDB_id=1394 DSB67_RS13765 WP_005432827.1 2843948..2844592(+) (qstR) [Vibrio campbellii strain DS40M4]
MKKSAYARKLFLISMEEDAGKKVEALEKYTDIDIPVISTQALMEANPKHRNKILLIDFSEHKELVLSIKNLPLIWKNFET
VVFNVPKRLTTDELLAFGQLKGIFYEESSLEQIGEGLKEIINGQNWLPRNVTSQLLHYYRNVISTHTAPATVDLTIRELQ
VLRCLQAGASNNQMAEELFVSEFTIKSHLYQIFKKLSVKNRVQAIAWADQNLMS

Nucleotide


Download         Length: 645 bp        

>NTDB_id=1394 DSB67_RS13765 WP_005432827.1 2843948..2844592(+) (qstR) [Vibrio campbellii strain DS40M4]
GTGAAAAAATCGGCTTATGCGAGAAAACTTTTTCTAATCAGCATGGAAGAGGATGCTGGGAAAAAGGTCGAAGCTCTGGA
GAAGTATACAGATATCGACATCCCCGTCATTTCGACCCAAGCACTGATGGAAGCAAACCCGAAGCACCGCAATAAAATCT
TGCTTATCGACTTCAGTGAACACAAAGAGTTGGTTCTATCGATAAAAAACCTTCCTCTGATTTGGAAGAATTTCGAAACA
GTCGTGTTCAACGTTCCTAAACGTTTGACGACAGATGAGCTATTGGCGTTTGGTCAATTGAAAGGCATCTTCTATGAAGA
GAGTTCTCTTGAGCAGATTGGCGAAGGGTTAAAGGAAATCATTAATGGTCAAAACTGGCTGCCCCGTAATGTCACCAGTC
AACTTCTCCATTATTATCGTAACGTCATTAGTACCCACACAGCTCCTGCCACCGTCGATTTGACCATTCGTGAACTGCAA
GTACTTCGCTGCCTGCAAGCTGGCGCATCAAACAATCAAATGGCCGAAGAGTTATTCGTGAGCGAATTCACTATCAAGTC
TCACCTGTATCAGATTTTTAAGAAGCTTTCGGTCAAAAACCGTGTACAAGCGATTGCTTGGGCTGACCAAAACCTGATGT
CATAA

Domains

Predicted by InterproScan.

Bacterial regulatory proteins, luxR family (GerE)

(154-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  qstR Vibrio parahaemolyticus RIMD 2210633

89.252

100

0.893
  qstR Vibrio cholerae strain A1552

52.336

100

0.523

Multiple sequence alignment    



References

[1] Chelsea A Simpson et al. (2019) Diversity in Natural Transformation Frequencies and Regulation across Vibrio Species. MBio 10(6):e02788-19. [PMID: 31848285]