Detailed information    

experimental Experimentally validated

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACIAD_RS01680 Genome accession   NC_005966
Coordinates   353373..354599 (-) Length   408 a.a.
NCBI ID   WP_004920476.1    Uniprot ID   Q6FF46
Organism   Acinetobacter baylyi ADP1     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

PilC is a member of the conserved PilC/GspF family of integral membrane proteins, which are found in type II secretion systems (T2SS), T4P, archaeal flagella and natural transformation systems. Studies have shown that GspF proteins interact with the IM assembly platform via PilO and with cytoplasmic motor ATPases thereby linking the ATPase-dependent energy release to pilus/pseudopilus assembly.


Genomic Context

Location: 348373..359599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS01650 (ACIAD0355) - 348407..349714 (+) 1308 WP_004920495.1 tetratricopeptide repeat protein -
  ACIAD_RS01655 (ACIAD0356) - 349793..350119 (+) 327 WP_004920494.1 pyrimidine/purine nucleoside phosphorylase -
  ACIAD_RS01660 (ACIAD0357) rlmB 350219..350965 (+) 747 WP_004920490.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACIAD_RS01665 (ACIAD0358) - 351006..351923 (+) 918 WP_004920488.1 DMT family transporter -
  ACIAD_RS01670 (ACIAD0359) coaE 351920..352507 (-) 588 WP_004920482.1 dephospho-CoA kinase -
  ACIAD_RS01675 (ACIAD0360) pilD 352513..353373 (-) 861 WP_004920478.1 prepilin peptidase Machinery gene
  ACIAD_RS01680 (ACIAD0361) pilC 353373..354599 (-) 1227 WP_004920476.1 type II secretion system F family protein Machinery gene
  ACIAD_RS01685 (ACIAD0362) pilB 354628..356340 (-) 1713 WP_004920473.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACIAD_RS01690 (ACIAD0363) tpiA 356605..357402 (+) 798 WP_004920470.1 triose-phosphate isomerase -
  ACIAD_RS01695 (ACIAD0364) secG 357412..357741 (+) 330 WP_004920467.1 preprotein translocase subunit SecG -
  ACIAD_RS01715 (ACIAD0367) rimP 358821..359345 (+) 525 WP_004920465.1 ribosome maturation factor RimP -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45156.56 Da        Isoelectric Point: 9.9700

>NTDB_id=1016 ACIAD_RS01680 WP_004920476.1 353373..354599(-) (pilC) [Acinetobacter baylyi ADP1]
MAAKKTQVMPVFAYEGVDRKGIKLKGELPARNMALAKVTLRKQGITIRTIREKRKNIFEGLLKKKVKPLDIAIFTRQLAT
MMKAGVPLVQAFEIVAEGLENPAMRDVVLGLKNEVEGGNTFAGALRKYPQYFDNLFCSLIESGEQSGSLEIMLDRVAVYK
EKSEVLKQKIKKAMKYPATVIVVAIVVTIILMVKVVPVFKDLFTSFGADLPAFTQLVVNMSNWMQDYWFILILVIGAAIA
GFLEARKRSKRFRDQLDRLVLRLPIVGDLVYKAIIARYSRTLATTFAAGVPLIDALASTAGATNNTVYEQAVLKIREDVS
TGQQLNFAMRVSNRFPTMAIQMVAIGEESGALDEMLDKVANYYESEVDHAVDGLTSMMEPLIMAILGILVGGLVIAMYLP
IFQMGSVV

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1016 ACIAD_RS01680 WP_004920476.1 353373..354599(-) (pilC) [Acinetobacter baylyi ADP1]
ATGGCAGCAAAAAAAACGCAAGTAATGCCAGTATTTGCTTATGAAGGTGTCGACCGTAAAGGCATCAAGTTAAAAGGGGA
ATTACCTGCACGCAATATGGCATTGGCCAAAGTCACTTTGCGAAAACAAGGCATTACGATTCGCACCATACGTGAAAAAC
GTAAAAATATTTTTGAAGGTTTACTTAAAAAGAAAGTCAAACCACTCGATATTGCCATTTTTACTCGTCAACTTGCCACC
ATGATGAAGGCAGGTGTTCCATTGGTACAAGCATTTGAAATTGTAGCAGAAGGGTTAGAAAACCCAGCCATGCGCGATGT
AGTGCTTGGCTTAAAAAATGAAGTTGAAGGCGGCAATACCTTTGCTGGTGCTTTACGCAAGTATCCCCAGTATTTTGATA
ACTTATTCTGTTCCCTCATTGAATCAGGGGAGCAGTCGGGTTCTCTTGAAATTATGCTAGACCGTGTGGCCGTCTATAAA
GAAAAAAGTGAAGTTCTTAAGCAAAAAATTAAAAAAGCCATGAAATATCCTGCCACCGTCATTGTAGTTGCGATTGTGGT
AACCATTATTTTGATGGTAAAGGTTGTCCCTGTATTTAAAGACTTATTTACATCATTTGGTGCAGATTTACCTGCTTTTA
CGCAACTGGTGGTCAATATGTCAAACTGGATGCAAGATTACTGGTTTATTTTAATTCTGGTGATTGGGGCTGCAATTGCA
GGTTTCCTGGAAGCTAGAAAACGCAGTAAACGCTTTCGCGATCAACTTGATCGTTTGGTTCTCCGATTGCCCATTGTTGG
TGATCTGGTCTACAAGGCAATCATTGCCCGATACAGTCGCACTTTGGCCACAACTTTTGCTGCAGGTGTACCACTCATTG
ATGCGCTGGCCTCTACCGCAGGTGCCACGAATAATACTGTTTATGAACAGGCAGTGCTTAAAATCCGAGAAGATGTATCA
ACTGGACAACAACTTAACTTCGCTATGCGCGTATCCAATCGTTTTCCAACCATGGCTATCCAAATGGTAGCGATTGGTGA
AGAATCTGGAGCACTCGATGAAATGCTGGATAAAGTCGCAAATTATTATGAAAGTGAAGTCGATCATGCGGTCGATGGCC
TTACCTCCATGATGGAACCGCTGATTATGGCGATTTTGGGCATTTTAGTCGGTGGACTGGTTATTGCAATGTATCTTCCA
ATTTTCCAAATGGGCTCTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6FF46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baumannii D1279779

85.784

100

0.858
  pilC Pseudomonas stutzeri DSM 10701

60.688

100

0.61
  pilC Legionella pneumophila strain ERS1305867

53.086

99.754

0.53
  pilG Neisseria gonorrhoeae MS11

45.363

97.794

0.444
  pilG Neisseria meningitidis 44/76-A

45.113

97.794

0.441
  pilC Vibrio cholerae strain A1552

42.015

99.755

0.419
  pilC Vibrio campbellii strain DS40M4

42.462

97.789

0.415
  pilC Thermus thermophilus HB27

38.462

99.261

0.382

Multiple sequence alignment    



References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]