Detailed information NTG0001113

Experimentally validated

Overview

• Name: pilG
• Type: Machinery gene
• Locus tag: -
• Genome accession: U19580
• Coordinates: 219..1451 (+)
• Length: 410 a.a.
• NCBI ID: AAA85695.1
• Uniprot ID: -
• Organism: Neisseria meningitidis 44/76-A
• Function: assembly of type IV pilus DNA binding and uptake

Function

Chromosomal pilG insertion mutants were devoid of pili and displayed dramatically reduced competence for transformation.

Genomic Context

Location: 1..6451

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Locus_0	pilG	219..1451 (+)	1233	AAA85695.1	PilG	Machinery gene

Sequence

Protein

• Length: 410 a.a.
• Molecular weight: 45435.44 Da
• Isoelectric Point: 9.9375

>NTDB_id=1113 AAA85695.1 219..1451(+) (pilG) [Neisseria meningitidis 44/76-A]
MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARKKLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLS
TMINAGLPLMQAFEIVARGHGNPSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTVMDMSDFFVSYGWMVLIALGFAI
YRFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTIARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRV
IQGLSMTSGMRATELFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGWLSAMMEPIIILILGLVIGTLLVAMYL
PLFNLGNVVA*

Nucleotide

• Length: 1233 bp

>NTDB_id=1113 AAA85695.1 219..1451(+) (pilG) [Neisseria meningitidis 44/76-A]
ATGGCTAAAAACGGGGGATTTTCTTTGTTCGCAAAGAAAGAAAAACGCTTTATCTTTGAAGGCAGGCATTCCGCCTCCGA
CAAACTGGTCAACGGCGAAGTATCCGCGTTTACCGAAGAAGAGGCACGCAAAAAACTGGCAAAACGCGGCATCCGCCCGT
TGCAGATTACCCGTGTGAAAACAAGCTCCAAGCGCAAAATCACACAAGAAGACATTACCGTTTTCACCCGCCAGCTTTCC
ACGATGATTAATGCGGGCCTGCCGCTGATGCAGGCATTTGAAATCGTGGCGCGCGGACACGGCAACCCGTCTATGACGGA
AATGCTGATGGAAATCCGAGGCCAAGTGGAACAGGGCAGCTCGTTGAGCCGCGCATTCTCAAACCACCCGAAATATTTCG
ACCGCTTCTACTGCAATCTGGTTGCGGCGGGCGAAACGGGCGGTGTATTGGAAAGCCTGCTGGACAAATTGGCAATTTAC
AAGGAAAAAACCCAGGCCATCCGCAAAAAGGTAAAAACCGCGCTAACCTATCCGGTATCCGTCATCGCCGTCGCCATCGG
TTTGGTATTCGTGATGATGATTTTCGTACTGCCCGCCTTTAAAGAAGTTTACGCCAATATGGGCGCGGAGCTTCCCGCAC
TGACCCAAACAGTGATGGATATGTCCGACTTTTTCGTCTCATACGGCTGGATGGTGCTGATCGCACTGGGCTTTGCCATA
TACCGCTTCCTTAAATTGAAGGCGCGTTCGATTAAAATCCAACGGCGTATGGATGCCATACTGCTGCGTATGCCGATTTT
CGGAGACATTGTCCGCAAAGGGACGATTGCCCGCTGGGGCAGGACGACGGCAACGCTGTTTGCCGCCGGCGTGCCTTTGG
TCGATGTATTGGACTCCACTGCCGGCGCGGCGGGCAATTTAATCTATGAAGAAGCCACCCGGGAAATCCGTACGCGCGTC
ATCCAAGGTCTGTCTATGACTTCGGGGATGCGTGCGACGGAACTGTTCCCCAATATGATGTTGCAGATGTCCTCCATCGG
CGAGGAATCGGGTTCTTTGGACGATATGCTCAACAAAGCCGCCGAATTTTACGAAGACGAGGTGGACAATGCGGTCGGCT
GGCTGTCCGCTATGATGGAGCCGATTATTATTCTGATTTTAGGCTTGGTCATCGGCACGCTTCTGGTCGCTATGTATCTG
CCGCTGTTTAATTTGGGCAACGTGGTCGCCTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (281-401)

• Type II secretion system (T2SS), protein F (T2SSF) (75-198)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilG	Neisseria gonorrhoeae MS11	98.78	100	0.988
pilC	Acinetobacter baumannii D1279779	47.236	97.549	0.461
pilC	Pseudomonas stutzeri DSM 10701	44.665	99.506	0.444
pilC	Acinetobacter baylyi ADP1	45.113	97.794	0.441
pilC	Legionella pneumophila strain ERS1305867	45.088	97.783	0.441
pilC	Vibrio cholerae strain A1552	40.796	98.529	0.402
pilC	Vibrio campbellii strain DS40M4	38.235	100	0.383

References

• [1]: Stephan A Frye et al. (2015) The Inner Membrane Protein PilG Interacts with DNA and the Secretin PilQ in Transformation. PloS One 10(8):e0134954. [PMID: 26248334]
• [2]: Emma Lång et al. (2009) Identification of neisserial DNA binding components. Microbiology (Reading, England) 155(Pt 3):852-862. [PMID: 19246756]
• [3]: T Tønjum et al. (1995) Identification and characterization of pilG, a highly conserved pilus-assembly gene in pathogenic Neisseria. Molecular Microbiology 16(3):451-64. [PMID: 7565106]