Detailed information    

experimental Experimentally validated

Overview

Name   pilC   Type   Machinery gene
Locus tag   TT_RS02230 Genome accession   NC_005835
Coordinates   427762..428982 (+) Length   406 a.a.
NCBI ID   WP_011228203.1    Uniprot ID   Q5SK58
Organism   Thermus thermophilus HB27     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

A subcomplex in the IM comprising of PilMNOC forms the base for retraction and extension of the T4P structures comprising the major pilin PilA4 and the DNA translocator pseudopilus comprising PilA4 and the minor pilins PilA1–3.


Genomic Context

Location: 422762..433982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TT_RS02200 (TT_C0434) holA 422873..423751 (-) 879 WP_011172881.1 DNA polymerase III subunit delta -
  TT_RS02205 (TT_C0435) - 423786..424943 (-) 1158 WP_011172882.1 acyl-CoA dehydrogenase family protein -
  TT_RS02210 (TT_C0436) - 424953..425579 (-) 627 WP_011228202.1 GNAT family N-acetyltransferase -
  TT_RS02215 (TT_C0437) - 425613..426695 (+) 1083 WP_011172884.1 PIG-L deacetylase family protein -
  TT_RS02220 (TT_C0438) scpB 426639..427172 (-) 534 WP_008632201.1 SMC-Scp complex subunit ScpB -
  TT_RS02225 (TT_C0439) - 427176..427763 (-) 588 WP_011172885.1 L-threonylcarbamoyladenylate synthase -
  TT_RS02230 (TT_C0440) pilC 427762..428982 (+) 1221 WP_011228203.1 type II secretion system F family protein Machinery gene
  TT_RS02235 (TT_C0441) - 428957..429409 (-) 453 WP_011172887.1 EVE domain-containing protein -
  TT_RS02240 (TT_C0442) - 429478..429945 (+) 468 WP_011172888.1 hypothetical protein -
  TT_RS02245 (TT_C0443) - 429960..431315 (+) 1356 WP_011172889.1 FAD-binding oxidoreductase -
  TT_RS02250 (TT_C0444) - 431544..432491 (+) 948 WP_011172890.1 IS701-like element ISTth8 family transposase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44687.70 Da        Isoelectric Point: 9.7205

>NTDB_id=1029 TT_RS02230 WP_011228203.1 427762..428982(+) (pilC) [Thermus thermophilus HB27]
MPVYQYKARDRQGRLVEATIEAEDLRTAARLLRDRGLFVAEIKEPGKGLQAEVRIPALERGPGLKDLAIFSRQLATMLGA
GLTLLQALAILERQTENRKFREILKQVRTDVEGGMAFSEALSKHKIFSRLYVNLVRAGETSGGLDLILDRLASFLEKELE
LRGKIRSAMTYPVIVFVFAVGVAYFLLTGIVPQFAQILTDLGSELPLLTRFLIAVSDLLRAATLPLLLLAVALFFAYRWY
YGTPQGRRVIDRLKLRLPVFGNLNRKTAVARFSRTLALLLSSGVNIVEALDITKGTAGNSVVEEIVEAAKLKIQQGDPLN
LTLAQHPFVFPPMVSSMVAIGEETGALDTMLSKVADFYEREVDEAVASLTAAIEPLMIIFLGVIVGMIVAGMFLPLFKII
GTLSVQ

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1029 TT_RS02230 WP_011228203.1 427762..428982(+) (pilC) [Thermus thermophilus HB27]
ATGCCGGTCTACCAGTATAAGGCCCGGGACCGCCAGGGCCGTCTGGTGGAGGCCACCATTGAGGCCGAGGACCTCCGCAC
CGCCGCGAGGCTCCTCCGCGACCGGGGCCTCTTCGTGGCCGAGATCAAGGAGCCGGGGAAGGGCCTGCAGGCGGAGGTCC
GGATCCCCGCTTTAGAGCGGGGGCCGGGCCTGAAGGACCTCGCCATCTTCTCCCGGCAGCTCGCCACCATGCTGGGGGCG
GGCCTCACCCTCCTCCAGGCCCTCGCCATCCTGGAGCGCCAGACGGAAAACCGGAAGTTCCGGGAGATCCTCAAGCAGGT
GCGCACCGACGTGGAGGGGGGGATGGCCTTCTCCGAGGCCCTCTCCAAGCACAAGATCTTCTCCCGCCTCTACGTGAACC
TGGTGCGGGCGGGGGAGACCTCGGGCGGCCTGGACCTCATCCTGGACCGGCTGGCGAGCTTTCTGGAGAAGGAGCTGGAG
CTAAGGGGCAAGATCCGGAGCGCCATGACCTACCCGGTCATCGTCTTCGTTTTCGCCGTGGGCGTGGCCTACTTCCTCCT
CACGGGGATCGTGCCCCAGTTCGCCCAGATCCTCACGGATTTAGGCTCCGAGCTTCCCCTCCTCACCCGCTTCCTCATCG
CCGTCTCCGACCTTCTTAGGGCGGCCACCCTGCCCCTCCTCCTCCTCGCCGTGGCCCTCTTCTTCGCCTACCGCTGGTAC
TACGGCACCCCCCAGGGGCGTAGGGTCATTGACCGCCTTAAGCTCCGCCTCCCCGTCTTCGGCAACCTCAACCGCAAGAC
CGCCGTGGCCCGCTTCTCCCGCACCCTGGCCCTCCTTCTCTCCAGCGGGGTGAACATCGTGGAGGCCCTGGACATCACCA
AGGGGACCGCGGGCAACAGCGTGGTGGAGGAGATCGTGGAGGCCGCCAAGCTGAAGATCCAGCAGGGCGACCCCCTGAAC
CTCACCCTGGCCCAGCACCCCTTCGTCTTCCCGCCCATGGTGAGCTCCATGGTGGCCATCGGCGAGGAGACGGGGGCCCT
GGACACCATGCTCTCCAAGGTGGCCGACTTCTATGAGCGGGAGGTGGACGAGGCGGTGGCGAGCCTCACCGCGGCCATTG
AGCCCCTCATGATCATCTTCCTGGGCGTCATCGTGGGCATGATCGTGGCCGGGATGTTCCTGCCCCTCTTCAAGATCATC
GGGACCCTCTCCGTGCAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(272-395)

Type II secretion system (T2SS), protein F (T2SSF)

(70-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 2WHN

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baylyi ADP1

38.462

99.261

0.382
  pilC Pseudomonas stutzeri DSM 10701

36.908

99.012

0.365
  pilC Acinetobacter baumannii D1279779

36.658

98.768

0.362

Multiple sequence alignment    



References

[1] Deniz Yaman et al. (2024) Identification of subcomplexes and protein-protein interactions in the DNA transporter of Thermus thermophilus HB27. Biochimica Et Biophysica Acta. Biomembranes 1866(7):184363. [PMID: 38909880]
[2] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[3] Vijaykumar Karuppiah et al. (2010) Structure and oligomerization of the PilC type IV pilus biogenesis protein from Thermus thermophilus. Proteins 78(9):2049-57. [PMID: 20455262]
[4] Cornelia Schwarzenlander et al. (2009) The role of single subunits of the DNA transport machinery of Thermus thermophilus HB27 in DNA binding and transport. Environmental Microbiology 11(4):801-8. [PMID: 19396940]