Detailed information NTG0421863

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GCO85_RS07735
• Genome accession: NZ_CP048618
• Coordinates: 1705898..1706761 (+)
• Length: 287 a.a.
• NCBI ID: WP_011946525.1
• Uniprot ID: I7HJU8
• Organism: Legionella pneumophila strain ERS1305867
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1700898..1711761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCO85_RS07715 (GCO85_07725)	-	1701391..1701717 (-)	327	WP_011213802.1	DUF1820 family protein	-
GCO85_RS07720 (GCO85_07730)	-	1701900..1702643 (-)	744	WP_061636356.1	methyltransferase domain-containing protein	-
GCO85_RS07725 (GCO85_07735)	pilB	1702902..1704626 (+)	1725	WP_011946523.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GCO85_RS07730 (GCO85_07740)	pilC	1704632..1705852 (+)	1221	WP_011213805.1	type II secretion system F family protein	Machinery gene
GCO85_RS07735 (GCO85_07745)	pilD	1705898..1706761 (+)	864	WP_011946525.1	A24 family peptidase	Machinery gene
GCO85_RS07740 (GCO85_07750)	-	1706775..1707980 (-)	1206	WP_013101470.1	cyclic nucleotide-binding domain-containing protein	-
GCO85_RS07745 (GCO85_07755)	-	1708088..1708681 (-)	594	WP_011213808.1	hypothetical protein	-
GCO85_RS07750 (GCO85_07760)	-	1708697..1709152 (-)	456	WP_027226538.1	hypothetical protein	-
GCO85_RS07755 (GCO85_07765)	-	1709286..1710101 (+)	816	WP_011215545.1	pyruvate, water dikinase regulatory protein	-
GCO85_RS07760 (GCO85_07770)	-	1710221..1711669 (-)	1449	WP_027226537.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32800.45 Da
• Isoelectric Point: 7.8327

>NTDB_id=421863 GCO85_RS07735 WP_011946525.1 1705898..1706761(+) (pilD) [Legionella pneumophila strain ERS1305867]
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFQLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNIFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDLIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=421863 GCO85_RS07735 WP_011946525.1 1705898..1706761(+) (pilD) [Legionella pneumophila strain ERS1305867]
ATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCTGTTGGAAGTTT
GCTTAATGTGATAATCTACCGCTTACCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCAGCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGCCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTGTTAAGAGGACGCTGTTACCAATGCGATAGTCCAATTTCAATTCGCTA
CCCTTTTGTTGAGACTCTGACCACAGTTTTATCCTTATACGCCAGTTGGCATTTTGGGTTTACAATACAACTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTCATTGCTAATACCCAGAATATATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCCTATTTAGCCTTATGGTTATTCATTAATCTGTTTTATCTGATGACAGGTAAAGTAGGTATGGGACATGGGGATTTCA
AATTATTTGCAGCATTTGGTGCATGGCTTGGATGGATGTATTTACCATTAATTCTACTAATATCTTCAATAACCGGGGCT
ATCATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGCCCTTTTCTTTGCATTTC
TGGATTAATAGCTATGTTTTGGGGAGATTTAATTATCAATTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

3D structure

• AlphaFold DB: I7HJU8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.11	97.909	0.481
pilD	Acinetobacter nosocomialis M2	48.352	95.122	0.46
pilD	Acinetobacter baumannii D1279779	47.636	95.819	0.456
pilD	Vibrio campbellii strain DS40M4	45.683	96.864	0.443
pilD	Neisseria gonorrhoeae MS11	42.446	96.864	0.411