Detailed information NTG0001080

Experimentally validated

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FDQ49_RS18305
• Genome accession: NZ_CP040105
• Coordinates: 1689803..1690663 (+)
• Length: 286 a.a.
• NCBI ID: WP_022575844.1
• Uniprot ID: A0A2T7FRH3
• Organism: Acinetobacter nosocomialis M2
• Function: assembly of type IV pilus DNA binding and uptake

Function

We constructed strains containing mutations in the pilA, pilD, or pilT gene and then determined the transformation efficiency of each strain. The transformation efficiency for all mutant strains was below the level of detection in our assay.

Genomic Context

Location: 1684803..1695663

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FDQ49_RS07875 (FDQ49_07875)	secG	1685410..1685739 (-)	330	WP_002156913.1	preprotein translocase subunit SecG	-
FDQ49_RS07880 (FDQ49_07880)	tpiA	1685752..1686546 (-)	795	WP_022575842.1	triose-phosphate isomerase	-
FDQ49_RS07885 (FDQ49_07885)	pilB	1686837..1688549 (+)	1713	WP_004710829.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FDQ49_RS07890 (FDQ49_07890)	pilC	1688577..1689803 (+)	1227	WP_022575843.1	type II secretion system F family protein	Machinery gene
FDQ49_RS18305 (FDQ49_07895)	pilD	1689803..1690663 (+)	861	WP_022575844.1	A24 family peptidase	Machinery gene
FDQ49_RS07900 (FDQ49_07900)	coaE	1690665..1691261 (+)	597	WP_000874125.1	dephospho-CoA kinase	-
FDQ49_RS07905 (FDQ49_07905)	-	1691258..1692172 (-)	915	WP_022575845.1	DMT family transporter	-
FDQ49_RS07910 (FDQ49_07910)	rlmB	1692208..1692957 (-)	750	WP_002156969.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
FDQ49_RS07915 (FDQ49_07915)	-	1693059..1693385 (-)	327	WP_000099685.1	pyrimidine/purine nucleoside phosphorylase	-
FDQ49_RS07920 (FDQ49_07920)	-	1693462..1694772 (-)	1311	WP_022575846.1	tetratricopeptide repeat protein	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32107.52 Da
• Isoelectric Point: 7.1784

>NTDB_id=1080 FDQ49_RS18305 WP_022575844.1 1689803..1690663(+) (pilD) [Acinetobacter nosocomialis M2]
MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPRMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACHQP
IRWYQNIPLISWLVLRGKCGHCQHPISIRYPAIELLTMLCSLVVVMVFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=1080 FDQ49_RS18305 WP_022575844.1 1689803..1690663(+) (pilD) [Acinetobacter nosocomialis M2]
ATGCAAGACATCATTGCGTATTTTATTCAAAACTTAACTGCACTTTATATTGCTGTTGCACTCGTGAGCCTATGTATCGG
TAGCTTTCTTAATGTAGTGATTTACCGCACACCAAGAATGATGGAGCAAGATTGGCAGCAAGAATGCCAAATGTTACTCA
ATCCTGAGCAACCCATTATTGATCATGAGAAGCTAACTTTAAGTAAGCCTGCTTCATCATGCCCTGCATGCCATCAACCC
ATCCGTTGGTATCAAAATATCCCACTCATAAGCTGGCTAGTGTTAAGAGGAAAATGTGGTCATTGCCAGCACCCGATTAG
CATTCGCTATCCAGCCATCGAACTACTTACCATGCTATGTTCATTGGTGGTGGTTATGGTGTTTGGTCCAACTATACAAA
TGCTTTTTGGACTGGTCCTCACATGGGTACTGATAGCACTTACCTTTATTGATTTCGATACACAATTATTGCCTGATCGC
TTTACTCTACCTTTAGCTGCGCTTGGTTTAGGCATTAACACCTTTAATATCTACACCTCACCCAACTCGGCCATTTGGGG
TTACCTCATTGGTTTTCTATGTCTTTGGATTGTCTATTACTTATTTAAAGTAATTACTGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCAGCATTAGGTGCATGGATGGGGCCATTGATGCTGCCGTTAATTGTGTTATTGTCGTCTTTA
TTGGGTGCAATCATTGGCATCATCTTATTAAAGTTAAGAAATGACAATCAGCCTTTTGCGTTTGGGCCATATATTGCCAT
TGCAGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-128)

3D structure

• AlphaFold DB: A0A2T7FRH3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	98.252	100	0.983
pilD	Vibrio cholerae strain A1552	53.676	95.105	0.51
pilD	Vibrio campbellii strain DS40M4	52.03	94.755	0.493
pilD	Neisseria gonorrhoeae MS11	47.727	92.308	0.441

References

• [1]: Christian M Harding et al. (2013) Acinetobacter baumannii strain M2 produces type IV pili which play a role in natural transformation and twitching motility but not surface-associated motility. MBio 4(4):e00360-13. [PMID: 23919995]