Detailed information NTG0001111

Experimentally validated

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NGFG_RS09220
• Genome accession: NC_022240
• Coordinates: 1696927..1697787 (+)
• Length: 286 a.a.
• NCBI ID: WP_003689814.1
• Uniprot ID: A0AA44U9R8
• Organism: Neisseria gonorrhoeae MS11
• Function: assembly of type IV pilus DNA binding and uptake

Function

pilF and pilD gene products are required for competence for DNA transformation. Mutants lacking the pilD gene product, a pre-pilin peptidase, were unable to process the pre-pilin subunit into pilin and thus were non-piliated. pilF mutants processed pilin but did not assemble the mature subunit.

Genomic Context

Location: 1691927..1702787

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NGFG_RS09205 (NGFG_02117)	dapD	1692437..1693258 (-)	822	WP_003689804.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
NGFG_RS09210 (NGFG_02118)	pgi	1693468..1695111 (-)	1644	WP_003689806.1	glucose-6-phosphate isomerase	-
NGFG_RS09215 (NGFG_02119)	pilG	1695621..1696853 (+)	1233	WP_003689811.1	type 4 pilus assembly protein PilG	Machinery gene
NGFG_RS09220 (NGFG_02120)	pilD	1696927..1697787 (+)	861	WP_003689814.1	prepilin peptidase	Machinery gene
NGFG_RS09225 (NGFG_02121)	coaE	1697784..1698416 (+)	633	WP_003689816.1	dephospho-CoA kinase	-
NGFG_RS09230 (NGFG_02122)	-	1698409..1698618 (+)	210	WP_003696096.1	DNA gyrase inhibitor YacG	-
NGFG_RS09235 (NGFG_02123)	pilF	1698704..1700380 (-)	1677	WP_003689817.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NGFG_RS09245 (NGFG_02124)	-	1700718..1701164 (-)	447	WP_003689818.1	DUF441 domain-containing protein	-
NGFG_RS09250 (NGFG_02125)	-	1701168..1702142 (-)	975	WP_003689819.1	polyprenyl synthetase family protein	-
NGFG_RS09255 (NGFG_02126)	rplU	1702366..1702674 (+)	309	WP_003691838.1	50S ribosomal protein L21	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31332.34 Da
• Isoelectric Point: 8.9561

>NTDB_id=1111 NGFG_RS09220 WP_003689814.1 1696927..1697787(+) (pilD) [Neisseria gonorrhoeae MS11]
MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVFAKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVR

Nucleotide

• Length: 861 bp

>NTDB_id=1111 NGFG_RS09220 WP_003689814.1 1696927..1697787(+) (pilD) [Neisseria gonorrhoeae MS11]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCAGTTTTGGGGCTGCTGGTCGGCAGCTTCCTGAA
TGTCGTCATTTACCGCGTACCCGTTATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAACATTTAAACCTGCCGCTGA
CCGACGATGAAAGCCGTACCTTCAACCTGATGAAGCCGGATTCCTGCTGTCCCAAATGCCGTGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGTTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTGTTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACGCTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACATTACCC
TTGATCTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATAGTTCATTATGGCTCTTATGTGCAGTGTATAAACTGCTCACAGGAAAAACCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCTCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCGGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (134-242)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	44.792	100	0.451
pilD	Vibrio campbellii strain DS40M4	45.652	96.503	0.441
pilD	Acinetobacter nosocomialis M2	47.727	92.308	0.441
pilD	Acinetobacter baumannii D1279779	47.727	92.308	0.441

References

• [1]: N E Freitag et al. (1995) Characterization of the pilF-pilD pilus-assembly locus of Neisseria gonorrhoeae. Molecular Microbiology 16(3):575-86. [PMID: 7565116]