Detailed information    

experimental Experimentally validated

Overview

Name   pilA   Type   Machinery gene
Locus tag   RS_RS02795 Genome accession   NC_003295
Coordinates   602739..603245 (+) Length   168 a.a.
NCBI ID   WP_011000517.1    Uniprot ID   Q8Y1X9
Organism   Ralstonia pseudosolanacearum GMI1000     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

pilA mutants did not make the 17 kDa PilA protein and did not exhibit twitching motility. A pilA mutant did not exhibit polar attachment to tobacco suspension culture cells or to tomato roots; it was also not naturally competent for transformation.


Genomic Context

Location: 597739..608245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS_RS02770 (RS04912) - 598084..598710 (+) 627 WP_011000512.1 DUF2889 domain-containing protein -
  RS_RS02775 (RS04911) sucC 598777..599943 (+) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  RS_RS02780 (RS04910) sucD 600028..600909 (+) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  RS_RS02785 (RS04909) - 601059..601757 (+) 699 WP_011000515.1 TerC family protein -
  RS_RS02790 (RS04908) pilA 601951..602472 (+) 522 WP_011000516.1 pilin Machinery gene
  RS_RS02795 (RS04907) pilA 602739..603245 (+) 507 WP_011000517.1 pilin Machinery gene
  RS_RS02800 (RS04906) - 603327..605060 (+) 1734 WP_043876542.1 PglL family O-oligosaccharyltransferase -
  RS_RS02805 (RS04905) moaC 605289..605765 (-) 477 WP_011000519.1 cyclic pyranopterin monophosphate synthase MoaC -
  RS_RS02810 (RS04904) - 605859..607544 (+) 1686 WP_011000520.1 tetratricopeptide repeat protein -
  RS_RS02815 (RS04903) - 607559..608146 (-) 588 WP_011000521.1 DUF2946 family protein -

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 17053.72 Da        Isoelectric Point: 9.9749

>NTDB_id=1424 RS_RS02795 WP_011000517.1 602739..603245(+) (pilA) [Ralstonia pseudosolanacearum GMI1000]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQSDLSVGSSVFT
PTKNVANLTIAGTGTVPGQITVTYTTAAGGGTLALVPTAAGTALPVSSAPSGPIMWTCYAQNKAQAASSVAPSGTMSLAA
KYAPAECR

Nucleotide


Download         Length: 507 bp        

>NTDB_id=1424 RS_RS02795 WP_011000517.1 602739..603245(+) (pilA) [Ralstonia pseudosolanacearum GMI1000]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACGGTTCGTGCACGCGTGACGGAAGGTCTGTCGCTGG
CTGCGCAAGCCAAGGCGCTGGTGTCGGAAAACGCTGCCAATGCACAGTCTGACCTGTCGGTGGGTTCGTCGGTGTTCACG
CCCACCAAGAACGTTGCCAACTTGACGATTGCTGGTACGGGTACTGTTCCGGGTCAGATCACCGTCACCTATACGACGGC
AGCTGGCGGCGGCACGCTGGCTCTGGTTCCGACTGCGGCTGGTACTGCACTGCCGGTTAGCTCGGCACCGTCGGGCCCGA
TCATGTGGACCTGCTACGCTCAGAATAAGGCTCAGGCTGCTAGCAGCGTTGCTCCGAGCGGTACGATGTCGCTGGCAGCG
AAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-168)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8Y1X9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

49.689

100

0.588
  pilA2 Legionella pneumophila strain ERS1305867

49.068

100

0.581
  comP Acinetobacter baylyi ADP1

44.444

100

0.517
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.082

100

0.417
  pilA/pilA1 Eikenella corrodens VA1

34.857

100

0.396

Multiple sequence alignment    



References

[1] Yaowei Kang et al. (2002) Ralstonia solanacearum requires type 4 pili to adhere to multiple surfaces and for natural transformation and virulence. Molecular Microbiology 46(2):427-37. [PMID: 12406219]