Detailed information    

experimental Experimentally validated

Overview

Name   letS   Type   Regulator
Locus tag   GCO85_RS09730 Genome accession   NZ_CP048618
Coordinates   2153730..2156462 (+) Length   910 a.a.
NCBI ID   WP_080450539.1    Uniprot ID   -
Organism   Legionella pneumophila strain ERS1305867     
Function   regulate competence development   
Competence regulation

Function

Mutation of this gene caused a decrease in transformation. LetA/S TCS is involved in the regulation of competence upstream of the regulation controlled by the RocC/RocR system.


Genomic Context

Location: 2148730..2161462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS09710 (GCO85_09720) gstA 2148872..2149477 (-) 606 WP_061637035.1 glutathione transferase GstA -
  GCO85_RS09715 (GCO85_09725) - 2149674..2150669 (-) 996 WP_011214162.1 hypothetical protein -
  GCO85_RS09720 (GCO85_09730) - 2150710..2151972 (-) 1263 WP_061637037.1 serine hydrolase domain-containing protein -
  GCO85_RS09725 (GCO85_09735) gltX 2152219..2153631 (-) 1413 WP_061637039.1 glutamate--tRNA ligase -
  GCO85_RS09730 (GCO85_09740) letS 2153730..2156462 (+) 2733 WP_080450539.1 ATP-binding protein Regulator
  GCO85_RS09735 (GCO85_09745) - 2156559..2157803 (+) 1245 WP_011214166.1 6-phosphofructokinase -
  GCO85_RS09740 (GCO85_09750) pilA2 2157800..2158213 (-) 414 WP_013101573.1 pilin Machinery gene
  GCO85_RS09745 (GCO85_09755) pilA2 2158410..2158820 (-) 411 WP_014844212.1 pilin Machinery gene
  GCO85_RS09750 (GCO85_09760) - 2159123..2159440 (+) 318 WP_010947633.1 BolA/IbaG family iron-sulfur metabolism protein -
  GCO85_RS09755 (GCO85_09765) - 2159515..2160936 (+) 1422 WP_061637041.1 APC family permease -

Sequence

Protein


Download         Length: 910 a.a.        Molecular weight: 102970.50 Da        Isoelectric Point: 7.5330

>NTDB_id=1263 GCO85_RS09730 WP_080450539.1 2153730..2156462(+) (letS) [Legionella pneumophila strain ERS1305867]
MLKSIGIKYQLRITTLIPAFLVALLFAFFYNGLFGKDLKQHMSRLGEAYIRQLLPAAQYAMLRNDYRTLQGLINASTINP
EVKALAFYNADGRLIAYRGGKHSIHKPFNPPDYTGDYIESKQINPFTINFIAPITIPKFNLYSSTEFKELSTPKIFQADD
ILGWLSIDIDTQSLLIKRYQMLIVTIFITLFGLLMGLTIHYFLSKRIYMPIARLRRSMKQILSNEFETEIRVSSPGELGI
IEKGCAHLQRQYLNTVRDLNHHIEIATADLQQSLELLEEKNIELSLEKKKTEEKSRQKSEFIANMSHEIRTPMNGVIGFT
NVLLESKLDPLQLDYVKTIKSSAQDLLSIINDILDFSKIDAGKLNLDCIPLDIRGCIDEVLSLASPNAHKKGIDLIPITD
INVPKMVLGDPLRIKQIISNLVTNAVKFTDHGYVLIRTKIEQETDKDYTLLFAIADTGIGISPEDQTKLFTAFNQADTSI
TRRYGGSGLGLVICKKLCEEMHGRISLTSEINKGSTFSARIKVEKLVAYEIEKNQTHRFAHLKIICFDDNPLHLEAIGNG
LGFWGIEAIRVDSFNKLSRILTKHKDCKIAFINVNQGCERQAAELIAKHKQIPFVLISKWPINDFAALGARGFLYKPISI
QKLQDLIESIANENQTEKNTNQELDTLREQLRFLHPEILIAEDNPVNKMLLTSLLNNNANITTVDDGEMAVTACEDKKFD
MILLDLHMPKLNGLEAAKMIRQKSLMNKHSPIVLITASSSDLSSIDMKKSGVDFCFQKPIDEKQLLIQILRIVDKTKHAA
IDWQLCVQKVSGNQALAEEFLAKFIEELYKNREEFIELMHQKNVKGLADLAHKLHGACCFCGVPILQKRVAQLEKLARRT
ANADNLAEAFTDLIQSIDAVISEYENQYSQ

Nucleotide


Download         Length: 2733 bp        

>NTDB_id=1263 GCO85_RS09730 WP_080450539.1 2153730..2156462(+) (letS) [Legionella pneumophila strain ERS1305867]
ATGTTGAAAAGTATTGGTATTAAATATCAGCTAAGAATCACTACTCTTATTCCTGCCTTTTTAGTCGCCTTGCTTTTTGC
TTTTTTTTATAACGGCCTATTTGGTAAAGATCTAAAGCAGCATATGTCCAGACTGGGTGAAGCCTATATTAGGCAATTAC
TTCCAGCAGCCCAGTATGCCATGCTTCGCAATGATTACCGTACATTGCAGGGTTTAATCAATGCCTCAACCATTAACCCG
GAGGTAAAAGCTTTGGCATTTTATAATGCTGATGGCCGATTAATTGCTTATCGTGGTGGCAAGCACTCTATTCATAAACC
CTTCAATCCACCTGACTATACAGGTGACTACATTGAAAGTAAGCAAATTAACCCATTCACCATTAATTTTATAGCGCCGA
TTACTATCCCAAAATTCAATTTGTACTCCAGTACAGAATTCAAAGAGCTTTCTACGCCTAAAATATTCCAGGCTGACGAT
ATATTAGGCTGGTTATCCATTGATATTGATACACAATCACTACTTATAAAACGCTATCAAATGCTCATCGTTACCATTTT
TATTACTCTATTCGGTTTACTGATGGGTTTAACAATTCATTACTTCTTGTCTAAACGAATTTACATGCCTATTGCTCGAT
TGCGTCGAAGTATGAAGCAAATTTTAAGTAATGAATTTGAAACTGAAATTCGTGTTTCAAGTCCAGGAGAATTAGGAATA
ATTGAAAAAGGTTGCGCTCATTTACAACGACAATATTTAAATACAGTTCGTGATTTGAATCACCACATTGAAATCGCCAC
AGCAGATCTGCAACAAAGTCTAGAACTTCTGGAAGAAAAAAACATTGAGCTATCACTGGAAAAGAAAAAAACTGAAGAAA
AAAGTCGACAAAAATCAGAATTTATTGCCAACATGAGTCATGAAATTCGCACGCCAATGAATGGCGTGATTGGTTTCACA
AATGTTTTATTGGAAAGCAAGCTGGACCCTTTACAACTGGACTACGTTAAAACGATCAAATCATCAGCGCAGGATTTGTT
AAGTATCATCAATGATATTCTGGATTTTTCTAAAATTGATGCGGGCAAATTAAATCTTGATTGCATCCCGCTAGACATAA
GAGGTTGCATTGATGAGGTTTTATCCCTGGCAAGCCCCAATGCGCACAAAAAAGGAATCGATTTAATCCCGATTACAGAC
ATCAATGTGCCCAAAATGGTATTGGGTGACCCTTTAAGAATCAAACAAATCATCAGTAATCTGGTGACCAATGCAGTAAA
ATTTACAGATCATGGATACGTTCTTATACGCACGAAAATCGAGCAAGAAACAGATAAAGATTATACCTTGCTATTTGCCA
TTGCGGATACGGGAATAGGTATTTCACCAGAGGATCAAACGAAACTGTTTACTGCCTTCAATCAGGCAGACACCAGCATC
ACACGCCGTTACGGTGGCTCAGGATTAGGCTTGGTTATATGTAAAAAACTATGTGAAGAAATGCATGGACGTATTAGTCT
AACCAGTGAAATCAACAAAGGCTCCACTTTTAGCGCACGCATTAAAGTTGAAAAACTTGTTGCTTATGAGATTGAAAAAA
ATCAAACTCATCGTTTCGCCCATTTAAAAATCATTTGCTTTGATGACAATCCCCTCCATTTGGAAGCAATAGGTAATGGC
TTGGGATTTTGGGGTATAGAGGCTATACGCGTTGACTCATTTAATAAACTTTCCCGAATATTGACAAAACATAAAGATTG
CAAAATTGCCTTTATTAACGTCAATCAAGGATGTGAGCGACAAGCTGCTGAACTCATTGCCAAACATAAGCAAATCCCTT
TCGTGTTAATTTCCAAATGGCCTATTAATGATTTTGCAGCGCTTGGTGCCAGGGGTTTTCTTTACAAACCTATTAGTATC
CAAAAGCTTCAGGATCTGATTGAATCGATAGCCAATGAAAACCAAACCGAGAAAAATACGAACCAGGAACTGGATACTTT
ACGCGAACAGCTTCGTTTTCTTCATCCAGAGATACTGATTGCAGAAGACAACCCTGTCAATAAAATGCTATTAACATCCC
TATTAAACAATAATGCCAATATAACAACGGTTGACGATGGCGAAATGGCCGTTACTGCTTGTGAGGATAAAAAATTTGAC
ATGATTTTACTTGATCTGCATATGCCAAAACTTAATGGTTTGGAAGCAGCCAAAATGATACGTCAAAAATCCCTAATGAA
TAAACACTCCCCTATCGTTCTCATTACCGCCAGTAGCAGTGATCTGAGTTCCATCGATATGAAAAAATCCGGAGTTGATT
TTTGTTTTCAAAAACCTATAGATGAAAAGCAATTGTTAATCCAAATTCTTCGCATCGTTGATAAAACCAAACATGCTGCC
ATCGATTGGCAATTATGCGTTCAGAAAGTTTCAGGTAATCAAGCGCTTGCTGAAGAGTTCCTCGCCAAATTCATAGAAGA
ATTATATAAGAATCGTGAAGAGTTTATTGAGTTAATGCATCAAAAAAACGTAAAGGGCCTGGCTGATTTGGCTCATAAAT
TACATGGCGCCTGTTGTTTTTGTGGTGTACCTATTTTACAAAAGAGAGTAGCTCAATTAGAGAAGCTTGCAAGACGAACA
GCTAATGCTGATAATTTAGCTGAAGCCTTTACCGATTTAATACAAAGTATTGATGCAGTGATTAGTGAATATGAAAATCA
GTATTCACAATAA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(298-362)

Hpt domain (Hpt)

(819-899)

Single cache domain 4 (sCache_4)

(36-180)

Response regulator receiver domain (Response_reg)

(678-787)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(409-524)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]