Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PAKAF_RS23875 Genome accession   NZ_LR657304
Coordinates   5178519..5180219 (+) Length   566 a.a.
NCBI ID   WP_009875880.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa PAK     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5173519..5185219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAKAF_RS23850 (PAKAF_04793) ampD 5173544..5174110 (-) 567 WP_016253886.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PAKAF_RS23855 (PAKAF_04794) - 5174261..5176516 (-) 2256 WP_016253887.1 DUF1631 domain-containing protein -
  PAKAF_RS23860 (PAKAF_04795) nadC 5176802..5177650 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PAKAF_RS23870 (PAKAF_05939) pilA 5177838..5178290 (-) 453 WP_024947914.1 pilin Machinery gene
  PAKAF_RS23875 (PAKAF_04797) pilB 5178519..5180219 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PAKAF_RS23880 (PAKAF_04798) pilC 5180223..5181443 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  PAKAF_RS23885 (PAKAF_04799) pilD 5181447..5182319 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PAKAF_RS23890 (PAKAF_04800) coaE 5182316..5182927 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  PAKAF_RS23895 (PAKAF_04801) yacG 5182924..5183124 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PAKAF_RS23900 (PAKAF_04802) - 5183161..5183370 (-) 210 WP_003094660.1 hypothetical protein -
  PAKAF_RS23905 (PAKAF_04803) - 5183476..5184165 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  PAKAF_RS23910 (PAKAF_04804) - 5184162..5184632 (-) 471 WP_003094664.1 hypothetical protein -
  PAKAF_RS23915 (PAKAF_04805) - 5184629..5185054 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62383.27 Da        Isoelectric Point: 5.5590

>NTDB_id=1128568 PAKAF_RS23875 WP_009875880.1 5178519..5180219(+) (pilB) [Pseudomonas aeruginosa PAK]
MNDSIQLSGLSRQLVQANLLDEKTALQAQTQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDRESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDAANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFENATDGLAGLDDVDLE
GLDVGVKETSGQEDTGAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1128568 PAKAF_RS23875 WP_009875880.1 5178519..5180219(+) (pilB) [Pseudomonas aeruginosa PAK]
ATGAACGACAGCATCCAACTGAGCGGTCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCCTGCA
GGCCCAGACGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGACAGGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATTCCCCTGTGGCGGCGGGGCAACAAGTTGTTCGTAGGCATCTC
CGACGCCGCCAACCACCAGGCCATCAACGACGTCCAGTTCAGCACTGGCCTGACGACCGAGGCCATTCTGGTCGAGGACG
ACAAGCTCGGCCTTGCCATCGACAAGCTATTCGAGAATGCAACCGACGGCTTGGCAGGTCTCGATGACGTGGACCTGGAA
GGACTGGATGTAGGCGTCAAGGAAACGTCCGGACAAGAAGATACTGGCGCGGAGGCAGACGACGCTCCCGTCGTCCGCTT
CGTCAACAAGATGCTGCTGGACGCTATCAAAGGCGGGTCTTCCGACTTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTTCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTGGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTGTACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTACCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGACGTACCGAAGGAAACCCTGCTTCACGAAGGTTTCCCAGAA
GAGTTGATCGGCACCTTCAAGCTGTATTCACCAGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.581
  pilB Acinetobacter baylyi ADP1

57.371

99.47

0.571
  pilB Legionella pneumophila strain ERS1305867

54.77

100

0.548
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50.444

99.47

0.502
  pilB Vibrio cholerae strain A1552

50

100

0.5
  pilB Vibrio campbellii strain DS40M4

49.113

99.647

0.489
  pilF Thermus thermophilus HB27

39.855

97.527

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.417

93.11

0.376

Multiple sequence alignment