Detailed information NTG0001070

Experimentally validated

Overview

• Name: comA/comEC
• Type: Machinery gene
• Locus tag: ABD1_RS13145
• Genome accession: NC_020547
• Coordinates: 2784187..2786619 (-)
• Length: 810 a.a.
• NCBI ID: WP_000472713.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii D1279779
• Function: ssDNA transport through the inner membrane DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.

Genomic Context

Location: 2779187..2791619

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABD1_RS13120 (ABD1_25580)	purM	2779510..2780580 (+)	1071	WP_000071984.1	phosphoribosylformylglycinamidine cyclo-ligase	-
ABD1_RS13125 (ABD1_25590)	purN	2780577..2781206 (+)	630	WP_000975522.1	phosphoribosylglycinamide formyltransferase	-
ABD1_RS13130 (ABD1_25600)	-	2781270..2782079 (-)	810	WP_000114074.1	alpha/beta fold hydrolase	-
ABD1_RS13135 (ABD1_25610)	sppA	2782098..2783114 (-)	1017	WP_001286611.1	signal peptide peptidase SppA	-
ABD1_RS13140 (ABD1_25620)	-	2783251..2784234 (+)	984	WP_001984639.1	lysophospholipid acyltransferase family protein	-
ABD1_RS13145 (ABD1_25630)	comA/comEC	2784187..2786619 (-)	2433	WP_000472713.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABD1_RS13150 (ABD1_25640)	lolD	2786705..2787391 (-)	687	WP_000049406.1	lipoprotein-releasing ABC transporter ATP-binding protein LolD	-
ABD1_RS13155 (ABD1_25650)	-	2787384..2788619 (-)	1236	WP_001984636.1	lipoprotein-releasing ABC transporter permease subunit	-
ABD1_RS18565	-	2788757..2788837 (+)	81	Protein_2625	PilZ domain-containing protein	-
ABD1_RS13160 (ABD1_25660)	-	2788867..2789775 (-)	909	WP_001140078.1	DMT family transporter	-
ABD1_RS13165 (ABD1_25670)	-	2789786..2790271 (-)	486	WP_001985538.1	YaiI/YqxD family protein	-
ABD1_RS13170 (ABD1_25680)	-	2790528..2791469 (+)	942	WP_000795874.1	hypothetical protein	-

Sequence

Protein

• Length: 810 a.a.
• Molecular weight: 92549.84 Da
• Isoelectric Point: 8.6332

>NTDB_id=1070 ABD1_RS13145 WP_000472713.1 2784187..2786619(-) (comA/comEC) [Acinetobacter baumannii D1279779]
MFKIILLGWIGGIALMGIDFPLIMQYEKVGEALLLLAFIFYLYKRPMFVDRPFLKAVFCLLCTTSLFMVGYHYAEKALIE
RLEQRETDTRNLDIIVYINRLSEEKDNKVQQTAQVLNLSKEPVNWLLYLKSNNQNLLKNNQNLELGHYYRISGKTRPAHS
YATPGAFDQEKWFIQRNIMSGFNVRYIEPLSLDEIYRLGYQQHLKEQQSFSNSFRLNIEKLRLTFRQILNSSSLQQKGLI
LALLTGDESLLSDETQLQFKQLGISHLLAISGPHVLIFAIMLSWAYHQFISRYYPQIYLWKPKQVLIAVPCCLGVLIYTA
FVGFEIPALRTFLSVLIFIGFLLLKQPIKPFTLLVYSASLLLLMDPFSVLSAGFWLSYGACFILLRIYQTIAQLPEQHFL
SLSSKMIFMSKVLIESQGKIFIALSPLTLLFFQQISWVAPLTNIIAVPIVGSVIVPLNIMAACAWFVIKPFGNMLFHFND
MLLSILLSCLGLLEKLSLTLQGISLTPLSLLAISCAIIILFLPKGILPKTWGILCCLPLVIMNKTSQQIQLNILDVGQGQ
AIFLQHPEQNWLIDTGGSYDEKIFSIGQNVVVPFLRQQGVRQLDHVVLSHLDQDHSGAFPLIQQEIPVKQLISNEQLPND
LKQPFQYCHQGQQWHYPELDIQILWPKEKDLAFVTSNQNQYSCVVYLQFKKVGGYQNFLIMGDAGWEAEYELLKDYPNLK
IDVLVLGHHGSKHSSAYDFLATLKPKLAIASAGFDNRYGHPSQQVIARLKALHIPLKSTVEQGTLSFVLENHKIVLHDRR
LDRLWLSRGF

Nucleotide

• Length: 2433 bp

>NTDB_id=1070 ABD1_RS13145 WP_000472713.1 2784187..2786619(-) (comA/comEC) [Acinetobacter baumannii D1279779]
ATGTTTAAGATTATTCTATTGGGGTGGATTGGCGGTATTGCATTGATGGGAATAGATTTCCCTTTAATCATGCAATATGA
AAAAGTGGGCGAGGCTCTACTGTTACTTGCCTTTATTTTTTATCTTTATAAACGACCAATGTTCGTTGATCGACCATTTT
TAAAGGCAGTGTTTTGCTTATTATGTACAACAAGTCTTTTTATGGTTGGTTACCACTATGCTGAAAAAGCACTGATTGAA
CGATTAGAACAAAGAGAAACGGATACCCGAAATCTCGACATTATTGTCTATATAAACCGTTTAAGTGAAGAAAAAGATAA
TAAGGTTCAACAAACTGCACAAGTTCTAAATCTTTCTAAAGAACCGGTGAATTGGTTGCTATATTTAAAAAGTAATAATC
AAAATTTATTAAAGAATAATCAGAATCTTGAATTAGGTCACTATTATCGAATATCTGGAAAAACAAGACCTGCGCATAGT
TATGCCACCCCAGGAGCTTTTGATCAGGAAAAGTGGTTTATTCAGCGAAATATTATGTCTGGTTTTAACGTGAGATATAT
TGAGCCTTTAAGTCTCGATGAAATCTATCGATTGGGCTATCAGCAACATTTAAAAGAACAACAGTCTTTTTCCAATAGTT
TTCGTTTAAATATAGAAAAACTTCGCTTAACTTTTAGGCAAATATTAAACAGCTCATCTCTACAGCAAAAGGGTTTAATT
TTAGCTTTGCTGACAGGTGATGAAAGCCTTTTATCAGATGAAACTCAACTACAGTTCAAACAATTAGGAATTAGTCATTT
ATTGGCGATCTCAGGCCCACATGTGCTCATTTTTGCCATTATGTTATCTTGGGCATATCATCAATTTATCAGTCGTTATT
ATCCTCAAATTTACTTATGGAAACCGAAACAGGTTTTGATAGCTGTACCATGCTGCCTTGGTGTTTTAATTTATACTGCA
TTTGTAGGTTTCGAAATTCCTGCACTACGAACATTCTTATCAGTCCTTATATTTATTGGATTTCTATTATTAAAACAACC
TATTAAACCTTTTACATTACTCGTATATAGCGCAAGTCTACTGTTGCTAATGGACCCGTTTAGTGTACTTTCTGCAGGTT
TTTGGCTGTCTTATGGGGCATGCTTTATTTTATTAAGAATTTACCAAACTATAGCGCAGCTACCTGAGCAACATTTTTTG
AGTCTGAGTTCAAAAATGATTTTTATGAGTAAGGTATTAATTGAATCTCAAGGCAAAATATTTATTGCATTGAGTCCTTT
AACCTTACTTTTCTTTCAACAAATTTCTTGGGTTGCTCCATTAACCAATATTATTGCCGTGCCTATTGTTGGTAGTGTTA
TTGTCCCTTTAAACATCATGGCTGCTTGCGCATGGTTTGTAATAAAACCATTTGGAAATATGCTTTTTCATTTCAATGAT
ATGTTGCTCAGCATATTGCTGAGTTGTTTGGGCTTATTAGAAAAACTCTCTTTAACATTACAAGGTATAAGCTTGACGCC
ACTGTCTTTATTAGCGATTAGTTGTGCCATTATTATTTTATTTTTACCTAAAGGAATTCTGCCCAAAACTTGGGGAATAT
TATGTTGTTTACCCTTAGTTATTATGAACAAAACGAGTCAGCAAATTCAGCTTAATATTTTAGATGTTGGGCAGGGGCAG
GCTATTTTTCTACAACATCCCGAACAAAACTGGTTAATTGACACAGGCGGTTCTTACGATGAAAAAATATTTAGTATTGG
ACAAAATGTTGTAGTGCCTTTTCTACGTCAGCAAGGCGTAAGACAACTAGATCATGTTGTGCTATCCCATCTTGATCAAG
ACCATAGTGGCGCGTTTCCTCTTATTCAACAAGAGATTCCTGTAAAGCAGCTTATTTCGAATGAACAATTACCAAATGAT
TTAAAGCAACCATTCCAATATTGCCATCAAGGGCAACAATGGCATTATCCTGAGTTAGATATTCAAATTTTATGGCCTAA
AGAAAAAGATCTGGCTTTTGTTACTTCTAACCAGAATCAATATTCTTGTGTTGTATATCTTCAATTTAAAAAAGTTGGTG
GTTACCAAAATTTTCTTATTATGGGCGATGCTGGATGGGAAGCTGAATACGAGTTATTAAAAGATTATCCTAACTTGAAG
ATAGATGTGTTAGTGCTAGGGCATCATGGAAGTAAGCACAGTTCGGCTTATGATTTCTTGGCGACCTTAAAACCTAAACT
GGCCATTGCATCGGCAGGGTTTGATAACCGTTATGGCCATCCTAGCCAACAAGTTATAGCACGTTTAAAAGCTCTACATA
TTCCGCTAAAAAGCACTGTGGAACAAGGAACCTTAAGTTTTGTGCTGGAAAACCACAAAATAGTTTTACATGACCGACGT
TTGGATAGGCTCTGGTTGAGTAGAGGTTTTTAA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (243-522)

• Metallo-beta-lactamase superfamily (Lactamase_B) (555-639)

• Domain of unknown function (DUF4131) (DUF4131) (33-188)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comA/comEC	Acinetobacter baumannii strain A118	97.407	100	0.974
comA/comEC	Acinetobacter baylyi ADP1	50.185	100	0.502

References

• [1]: Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB₂. Frontiers in Microbiology 12:738034. [PMID: 35126321]