Detailed information    

experimental Experimentally validated

Overview

Name   comA/comEC   Type   Machinery gene
Locus tag   ACIAD_RS12005 Genome accession   NC_005966
Coordinates   2592109..2594529 (-) Length   806 a.a.
NCBI ID   WP_004928865.1    Uniprot ID   -
Organism   Acinetobacter baylyi ADP1     
Function   ssDNA transport through the inner membrane   
DNA binding and uptake

Function

The polytopic IM ComEC (ComA) protein forms an IM channel transporting the DNA through the IM.


Genomic Context

Location: 2587109..2599529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS11980 (ACIAD2634) purM 2587442..2588512 (+) 1071 WP_004928857.1 phosphoribosylformylglycinamidine cyclo-ligase -
  ACIAD_RS11985 (ACIAD2635) purN 2588509..2589138 (+) 630 WP_004928858.1 phosphoribosylglycinamide formyltransferase -
  ACIAD_RS11990 (ACIAD2636) - 2589198..2590001 (-) 804 WP_004928859.1 alpha/beta fold hydrolase -
  ACIAD_RS11995 (ACIAD2637) sppA 2590013..2591029 (-) 1017 WP_004928863.1 signal peptide peptidase SppA -
  ACIAD_RS12000 (ACIAD2638) - 2591163..2592176 (+) 1014 WP_011182575.1 lysophospholipid acyltransferase family protein -
  ACIAD_RS12005 (ACIAD2639) comA/comEC 2592109..2594529 (-) 2421 WP_004928865.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACIAD_RS12010 (ACIAD2640) - 2594614..2595300 (-) 687 WP_004928867.1 ABC transporter ATP-binding protein -
  ACIAD_RS12015 (ACIAD2641) - 2595293..2596528 (-) 1236 WP_004928869.1 lipoprotein-releasing ABC transporter permease subunit -
  ACIAD_RS12020 (ACIAD2643) - 2596837..2597757 (-) 921 WP_004928871.1 DMT family transporter -
  ACIAD_RS12025 (ACIAD2644) - 2597766..2598233 (-) 468 WP_004928874.1 YaiI/YqxD family protein -
  ACIAD_RS12030 (ACIAD2645) - 2598456..2599394 (+) 939 WP_004928876.1 hypothetical protein -

Sequence

Protein


Download         Length: 806 a.a.        Molecular weight: 92065.15 Da        Isoelectric Point: 8.6568

>NTDB_id=1015 ACIAD_RS12005 WP_004928865.1 2592109..2594529(-) (comA/comEC) [Acinetobacter baylyi ADP1]
MFKIILLGWIAGIAMMGRTLECIEPFTCITGILTFLIWAIYFYKKRKHQIGSGLRALVILISASFLFGIGAQYADSALEE
RLYYKIMHSDTHEVIIYVHQMGESSDKGIKQVAEVLNIHPDVVNWVFYIDQSLLGAEKSSSLELGQYYRITAKTKPIHSY
ALEGAFDVEKWFVQQNWQATAQVYRIQTLSKQEVMSAGFYLHVKQQQTWRAQFLQSVEKLRLKFRNYFIDSSLSHHALLL
ALLTGDRSLLNPELEQQFQRFGISHLLAISGPHVLIFAAMVSWIFTRLIHRFKPEIYLSIPRQKLVLLPFLMCVILYCAF
VGFEIPALRTLFTVMLGSILLLIRHHLSAFSLLVLSASLLLWMDPFSILSAAFWLSYGACFILIRIYQDMTQAQQFHQTR
FTRGMQILKALVRSQWKIFVALMPLTLIIFKQISWIAPLSNLIAIPILGGIIVPLDIMAGCLWLFIPSLGNLLFYLNNSL
LELFIGILKGLDHLFNPQLQYYAFTPLLLICLTLAIVILFLPKGVVPKFWSVLCFVPLLIPSKMIRPTQISILDVGQGQS
IFIQDQTQRILLDTGGYYDESKFSIGKQVVVPFLKSQGVHQLDRVILTHLDQDHSGGLPAVLQLIPTRMIQANEEIKDLS
IPFSLCHSGQTWETSELKIQILSPKLENLQSTVFNRNEASCVMYLQLKQASDLKYFLMMGDAGWETEYQLLQQYPDLKVD
VLILGHHGSQHSSSYDFLKRYHPKIAIASAGFENGYGHPSAEVSNRLQDLNIPLLSTTDKGAIHFKFDSGNEQTEFYRQS
YKWLQR

Nucleotide


Download         Length: 2421 bp        

>NTDB_id=1015 ACIAD_RS12005 WP_004928865.1 2592109..2594529(-) (comA/comEC) [Acinetobacter baylyi ADP1]
ATGTTTAAAATTATTTTATTGGGGTGGATTGCTGGCATTGCCATGATGGGCAGGACACTGGAATGCATTGAACCATTTAC
ATGCATTACAGGAATTCTGACTTTTTTGATATGGGCTATTTATTTCTACAAAAAACGAAAGCATCAGATTGGTTCTGGAT
TAAGAGCACTTGTTATCTTAATTTCTGCCAGTTTCTTATTTGGTATAGGCGCACAATACGCAGATTCTGCTCTTGAGGAA
CGCTTGTATTATAAAATAATGCATTCGGATACACATGAGGTGATTATTTACGTTCATCAAATGGGTGAATCTTCAGATAA
AGGTATAAAACAAGTCGCTGAAGTACTCAACATTCATCCTGATGTCGTGAATTGGGTATTTTATATTGATCAGAGCTTAC
TGGGGGCTGAAAAAAGCAGTAGCCTTGAGCTTGGTCAGTATTATCGAATCACTGCTAAGACCAAGCCGATACATAGCTAT
GCACTTGAAGGTGCATTTGATGTGGAAAAATGGTTTGTTCAGCAAAATTGGCAAGCGACTGCCCAAGTTTATCGTATACA
AACCTTAAGCAAACAAGAGGTGATGAGTGCAGGTTTTTATTTACATGTAAAACAGCAACAGACATGGCGAGCACAGTTTT
TACAGTCTGTTGAAAAGCTCAGGCTTAAATTTCGGAACTACTTTATAGATTCATCCTTATCACATCATGCACTCTTACTG
GCGCTCCTAACAGGAGACCGCAGTTTACTCAATCCAGAGCTTGAGCAGCAATTTCAACGTTTTGGCATTTCTCACTTGTT
GGCGATTTCGGGACCCCATGTGCTCATTTTTGCTGCCATGGTAAGCTGGATTTTTACTCGATTGATTCATCGGTTTAAAC
CAGAAATTTACTTATCAATTCCACGTCAAAAATTGGTATTACTCCCTTTTTTAATGTGTGTGATTTTATATTGTGCTTTT
GTTGGCTTTGAAATCCCTGCACTTAGAACGCTGTTTACTGTAATGCTTGGCAGTATTTTACTTTTGATACGACATCATCT
AAGTGCTTTTTCACTTTTGGTCTTGAGTGCATCCTTACTGTTATGGATGGACCCCTTTAGTATTTTATCCGCTGCATTTT
GGCTATCTTATGGTGCATGTTTTATTTTGATCAGAATTTATCAAGATATGACACAAGCACAGCAGTTTCATCAGACTCGT
TTTACGCGTGGGATGCAGATTCTAAAAGCATTGGTTAGATCACAATGGAAAATTTTTGTCGCTCTGATGCCTTTGACGTT
AATTATTTTTAAGCAAATATCATGGATTGCTCCATTATCTAATCTAATCGCAATTCCGATATTGGGCGGTATTATTGTGC
CTCTAGATATTATGGCTGGATGCTTATGGCTATTCATTCCATCACTTGGAAATCTGTTGTTTTATTTAAATAATAGCCTG
CTCGAATTATTTATCGGAATCTTAAAAGGTCTTGATCATTTATTTAATCCTCAGCTTCAATATTATGCATTTACGCCGTT
ACTACTGATTTGTCTCACTTTAGCCATTGTGATCCTATTTCTACCCAAAGGCGTGGTTCCAAAATTCTGGTCAGTTTTAT
GCTTCGTTCCACTTTTAATTCCTTCTAAAATGATTCGGCCCACTCAGATTAGTATTTTAGATGTAGGACAAGGCCAGTCA
ATTTTTATTCAGGACCAAACCCAGCGTATTTTGCTAGATACGGGTGGATACTATGATGAAAGTAAATTTAGTATTGGCAA
ACAGGTCGTGGTTCCATTCTTGAAGTCTCAAGGTGTGCATCAACTGGATCGAGTAATTTTGACTCATCTTGATCAGGATC
ATAGTGGTGGACTACCTGCAGTATTGCAATTGATCCCAACGCGTATGATTCAGGCCAATGAAGAAATTAAAGATTTATCT
ATTCCATTCTCATTGTGTCATTCTGGTCAGACTTGGGAAACATCAGAGCTAAAAATCCAGATATTGTCACCAAAATTAGA
AAATTTACAGTCTACGGTATTTAATCGTAATGAAGCGTCTTGTGTGATGTATTTACAGCTTAAACAAGCATCAGATTTAA
AGTATTTTCTGATGATGGGGGATGCAGGCTGGGAAACCGAATATCAGCTATTACAACAATATCCCGATCTTAAAGTTGAT
GTTTTAATTTTGGGGCATCATGGCAGCCAACACAGTTCATCTTATGATTTTCTCAAGAGATATCATCCCAAAATTGCGAT
TGCTTCAGCAGGGTTTGAAAATGGATATGGTCATCCGAGTGCTGAGGTAAGCAATCGTCTGCAAGATTTGAATATTCCCT
TATTGAGTACAACTGATAAGGGAGCGATTCATTTTAAGTTTGATTCAGGGAATGAGCAGACAGAATTTTATCGTCAGTCG
TATAAGTGGTTGCAGCGTTAA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(242-523)

Domain of unknown function (DUF4131) (DUF4131)

(27-188)

Metallo-beta-lactamase superfamily (Lactamase_B)

(554-623)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comA/comEC Acinetobacter baumannii D1279779

50.185

100

0.502
  comA/comEC Acinetobacter baumannii strain A118

49.815

100

0.499

Multiple sequence alignment    



References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]