Detailed information    

experimental Experimentally validated

Overview

Name   comE   Type   Machinery gene
Locus tag   NGFG_RS09375 Genome accession   NC_022240
Coordinates   1720150..1720545 (-) Length   131 a.a.
NCBI ID   WP_003703428.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae MS11     
Function   DNA binding   
DNA binding and uptake

Function

Single-copy deletion of comE in N. gonorrhoeae did not have a measurable effect on competence, whereas serial deletions led to gradual decrease in transformation frequencies, reaching a 4 x 10(4)-fold reduction when all copies were deleted. Recombinant His-tagged ComE showed DNA binding activity, without any detectable sequence specificity.


Genomic Context

Location: 1715150..1725545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS09330 (NGFG_02140) - 1715453..1715698 (-) 246 WP_003689849.1 hypothetical protein -
  NGFG_RS09340 (NGFG_02141) - 1715810..1716238 (+) 429 WP_020997399.1 CopD family protein -
  NGFG_RS09345 (NGFG_02142) - 1716250..1716726 (+) 477 WP_003689851.1 CYTH domain-containing protein -
  NGFG_RS09350 (NGFG_02143) - 1716756..1717244 (-) 489 WP_003689852.1 dihydrofolate reductase -
  NGFG_RS09355 (NGFG_02144) aroG 1717312..1718367 (-) 1056 WP_003689853.1 3-deoxy-7-phosphoheptulonate synthase AroG -
  NGFG_RS15285 - 1718529..1718756 (-) 228 WP_153707886.1 hypothetical protein -
  NGFG_RS16350 (NGFG_02147) - 1718984..1719280 (+) 297 WP_003689859.1 hypothetical protein -
  NGFG_RS16355 (NGFG_02148) - 1719277..1719516 (+) 240 WP_003689861.1 membrane protein -
  NGFG_RS09375 (NGFG_02458) comE 1720150..1720545 (-) 396 WP_003703428.1 helix-hairpin-helix domain-containing protein Machinery gene
  NGFG_RS16160 - 1720650..1720766 (-) 117 Protein_1761 competence protein ComE -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 13796.61 Da        Isoelectric Point: 10.8790

>NTDB_id=1126 NGFG_RS09375 WP_003703428.1 1720150..1720545(-) (comE) [Neisseria gonorrhoeae MS11]
MSRGGATPYRFLLIRHIVARCGLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAEY
RAQNGAFKSVDDLIKVKGIGPAVLAKLKDQASVGAPAPKGPAKPVLPAVKK

Nucleotide


Download         Length: 396 bp        

>NTDB_id=1126 NGFG_RS09375 WP_003703428.1 1720150..1720545(-) (comE) [Neisseria gonorrhoeae MS11]
TTGAGCCGGGGCGGGGCAACGCCGTACCGGTTTTTGTTAATCCGCCATATCGTCGCAAGATGCGGTTTGTTGTTTGCAAC
CCTTAAAGGAAAAACCATGAAAAAAATGTTTGTATTGTTCTGTATGCTGTTCTCCTGCGCCTTCTCCCTTGCGGCGGTAA
ACATCAATGCGGCTTCGCAGCAGGAGCTGGAGGCGCTGCCGGGCATAGGCCCGGCGAAGGCGAAGGCCATTGCGGAATAC
CGCGCGCAAAACGGCGCGTTCAAGTCTGTGGACGATTTGATCAAGGTGAAGGGCATCGGTCCGGCGGTGCTGGCGAAGCT
GAAAGACCAGGCTTCCGTCGGCGCGCCCGCACCAAAAGGCCCCGCCAAACCGGTGCTGCCTGCGGTTAAAAAATAG

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(52-110)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Neisseria gonorrhoeae MS11

100

100

1
  comE Neisseria gonorrhoeae MS11

100

100

1
  comE Neisseria gonorrhoeae MS11

100

100

1
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

51.852

100

0.532
  comEA Vibrio campbellii strain DS40M4

40.86

97.895

0.4
  comEA Vibrio parahaemolyticus RIMD 2210633

38.889

95.745

0.372

Multiple sequence alignment    



References

[1] I Chen et al. (2001) ComE, a competence protein from Neisseria gonorrhoeae with DNA-binding activity. Journal of Bacteriology 183(10):3160-8. [PMID: 11325945]