Detailed information NTG0001181

Experimentally validated

Overview

• Name: tfoX
• Type: Regulator
• Locus tag: GTF75_RS08345
• Genome accession: NZ_CP047301
• Coordinates: 1854210..1854797 (-)
• Length: 195 a.a.
• NCBI ID: WP_001005366.1
• Uniprot ID: A0A0K9UTP3
• Organism: Vibrio cholerae O1 biovar El Tor strain DRC-193A
• Function: master regulator of natural competence Competence regulation

Function

TfoX is the master regulator of natural transformation in V. cholerae, activating competence genes in response to chitin. TfoX is also shown to invlove in the induction of T6SS.

Genomic Context

Location: 1849210..1859797

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GTF75_RS08325 (GTF75_08330)	-	1849438..1850703 (-)	1266	WP_000835928.1	YdgA family protein	-
GTF75_RS08330 (GTF75_08335)	-	1850926..1852881 (+)	1956	WP_001082992.1	ATP-binding protein	-
GTF75_RS08335 (GTF75_08340)	-	1852878..1853375 (+)	498	WP_001031964.1	response regulator	-
GTF75_RS08340 (GTF75_08345)	-	1853566..1854114 (+)	549	WP_001232641.1	hypothetical protein	-
GTF75_RS08345 (GTF75_08350)	tfoX	1854210..1854797 (-)	588	WP_001005366.1	TfoX/Sxy family DNA transformation protein	Regulator
GTF75_RS08350 (GTF75_08355)	-	1855175..1856095 (+)	921	WP_000019000.1	HDOD domain-containing protein	-
GTF75_RS08355 (GTF75_08360)	-	1856116..1857021 (+)	906	WP_000948497.1	lysine exporter LysO family protein	-
GTF75_RS08360 (GTF75_08365)	-	1857210..1857719 (+)	510	WP_000826868.1	hypothetical protein	-
GTF75_RS08365 (GTF75_08370)	panP	1857829..1859475 (+)	1647	WP_000253201.1	pyridoxal-dependent aspartate 1-decarboxylase PanP	-

Sequence

Protein

• Length: 195 a.a.
• Molecular weight: 22816.03 Da
• Isoelectric Point: 6.9718

>NTDB_id=1181 GTF75_RS08345 WP_001005366.1 1854210..1854797(-) (tfoX) [Vibrio cholerae O1 biovar El Tor strain DRC-193A]
MNEQQFFDYVTKFGAYQKRSMFGGIGLFQHDAMYVLVSEDRIFVRGGEELDPELLALGCEKYRHVKKQTTATVNYYDITE
LYEQHHPELDSIIERSIQFSVNQREFQRSAASRRLRDLPNMQLTLERMVKKAGIDDVETFMSLGAPEVFNKVRQAYGSDV
DVKLLWKFAGAIEGIHWKLLQEPRKRQLLESCQQR

Nucleotide

• Length: 588 bp

>NTDB_id=1181 GTF75_RS08345 WP_001005366.1 1854210..1854797(-) (tfoX) [Vibrio cholerae O1 biovar El Tor strain DRC-193A]
ATGAATGAGCAACAGTTTTTCGACTACGTAACTAAGTTTGGCGCCTACCAAAAACGCTCGATGTTTGGTGGTATTGGCTT
GTTTCAACACGACGCTATGTATGTGTTGGTCAGTGAAGACCGCATTTTTGTGCGTGGTGGAGAAGAGCTCGACCCTGAGC
TCTTGGCCTTAGGGTGCGAGAAATATCGCCATGTTAAAAAACAGACAACGGCAACCGTAAACTATTACGACATCACTGAA
CTGTATGAGCAACATCACCCTGAGCTAGACTCGATTATTGAACGCTCGATTCAGTTTTCTGTGAATCAGCGGGAATTTCA
ACGCTCAGCAGCCAGTCGTCGTCTACGGGATTTACCCAATATGCAACTGACTTTGGAGCGTATGGTAAAAAAAGCGGGCA
TTGACGATGTGGAAACTTTTATGAGCCTCGGTGCGCCAGAAGTGTTTAATAAGGTGCGTCAAGCTTATGGAAGTGATGTC
GATGTAAAACTACTGTGGAAGTTTGCGGGTGCGATTGAAGGCATTCACTGGAAACTGCTGCAAGAGCCGCGTAAGCGCCA
ACTGTTAGAAAGTTGTCAGCAGCGTTAA

Domains

Predicted by InterproScan.

• TfoX C-terminal domain (TfoX_C) (112-190)

• TfoX N-terminal domain (TfoX_N) (9-99)

3D structure

• AlphaFold DB: A0A0K9UTP3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
tfoX	Vibrio cholerae O1 biovar El Tor strain P27459	100	100	1
tfoX	Vibrio cholerae O1 biovar El Tor strain E7946	100	100	1
tfoX	Vibrio cholerae O1 biovar El Tor str. N16961	100	100	1
tfoX	Vibrio cholerae strain A1552	100	100	1
tfoX	Vibrio campbellii strain DS40M4	69.43	98.974	0.687
tfoX	Vibrio parahaemolyticus RIMD 2210633	68.394	98.974	0.677
tfoX	Aliivibrio fischeri ES114	59.043	96.41	0.569

References

• [1]: Sandrine Borgeaud et al. (2015) The type VI secretion system of Vibrio cholerae fosters horizontal gene transfer. Science (New York, N.Y.) 347(6217):63-7. [PMID: 25554784]
• [2]: Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]
• [3]: Karin L Meibom et al. (2005) Chitin induces natural competence in Vibrio cholerae. Science (New York, N.Y.) 310(5755):1824-7. [PMID: 16357262]