Detailed information    

experimental Experimentally validated

Overview

Name   comE   Type   Machinery gene
Locus tag   A4U84_RS02010 Genome accession   NZ_CP015099
Coordinates   460258..461520 (-) Length   420 a.a.
NCBI ID   WP_021113424.1    Uniprot ID   A0A836Z0A6
Organism   Glaesserella parasuis strain SC1401     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

The expression levels of candidate uptake components were significantly lower than in SC1401, respectively. These results revealed that the highly naturally competent strain SC1401 up-regulated most of the candidate natural competence-related regulons/components.


Genomic Context

Location: 455258..466520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4U84_RS01980 (A4U84_01980) - 455874..456734 (-) 861 WP_417935434.1 IS256 family transposase, variant Zn-binding type -
  A4U84_RS01985 (A4U84_01985) trxA 457074..457388 (-) 315 WP_015940165.1 thioredoxin -
  A4U84_RS01990 (A4U84_01990) - 457685..458314 (-) 630 WP_015940164.1 LysE family transporter -
  A4U84_RS01995 (A4U84_01995) - 458316..458786 (-) 471 WP_021113432.1 phosphatidylglycerophosphatase A family protein -
  A4U84_RS02000 (A4U84_02000) thiL 458789..459757 (-) 969 WP_062923924.1 thiamine-phosphate kinase -
  A4U84_RS02005 (A4U84_02005) nusB 459766..460179 (-) 414 WP_021112072.1 transcription antitermination factor NusB -
  A4U84_RS02010 (A4U84_02010) comE 460258..461520 (-) 1263 WP_021113424.1 type IV pilus secretin PilQ Machinery gene
  A4U84_RS02015 (A4U84_02015) comD 461530..461871 (-) 342 WP_235629440.1 hypothetical protein Machinery gene
  A4U84_RS02020 (A4U84_02020) comC 461916..462446 (-) 531 WP_021119496.1 hypothetical protein Machinery gene
  A4U84_RS02025 (A4U84_02025) comB 462443..462964 (-) 522 WP_062923926.1 hypothetical protein Machinery gene
  A4U84_RS02030 (A4U84_02030) comA 462940..463617 (-) 678 WP_021113429.1 hypothetical protein Machinery gene
  A4U84_RS02035 (A4U84_02035) - 463771..466326 (+) 2556 WP_062923927.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 46971.81 Da        Isoelectric Point: 7.3676

>NTDB_id=1381 A4U84_RS02010 WP_021113424.1 460258..461520(-) (comE) [Glaesserella parasuis strain SC1401]
MRYLFLLFFATFPVLANQQISLSIRNAPTAEIISYLAEETGKNITISDEIKDTKNFRVEKSHFDEILNSLIKTHQLNLKK
ENGIYYIHQAQEHKQHTTAQLVNALPKLITKTIKLHYSKASEVIESLTKGQGNLLSESGYLHFDDRSNSIIVKDSAASVK
NFTQLIESLDKPTEQIAIEARIVTISSEHLQQLGVRWGLFSPNENHYKLAGNLEGNGLTTNNLNVNFPVNPSASVALQIA
AINSRVLDLELTALESENNIEIIASPRLLTTDKKTASIKQGTEIPYAMYSKKEEITDIEFREAVLGLEVTPHISKQNQIL
LDLAISQNSPNNQINNTMVTIDKQEIKTQVLAKHGETIVLGGIFQHLIAKGEDKVPLLGSIPVIKRLFSQSQDKISKREL
VIFVTPYIVKSEKIGAEKQK

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=1381 A4U84_RS02010 WP_021113424.1 460258..461520(-) (comE) [Glaesserella parasuis strain SC1401]
ATGCGTTATTTATTTCTGCTCTTCTTTGCTACTTTTCCTGTATTAGCAAACCAACAAATTTCACTTTCGATAAGAAATGC
CCCAACGGCAGAGATTATTAGTTATTTGGCGGAGGAAACGGGAAAGAATATTACAATTTCGGATGAAATTAAAGATACAA
AAAATTTCAGAGTAGAAAAAAGCCATTTTGATGAGATATTAAATAGTTTAATCAAAACACATCAATTAAATTTAAAAAAA
GAAAATGGCATTTACTATATTCATCAAGCCCAAGAACATAAACAACATACTACGGCACAATTAGTTAATGCTCTGCCTAA
ATTAATCACCAAAACAATCAAGTTACACTATTCCAAAGCCTCTGAAGTTATAGAGTCTTTAACAAAAGGGCAAGGCAATT
TACTATCAGAAAGTGGTTATCTTCACTTTGATGATCGCAGTAATAGCATTATCGTCAAAGACAGTGCCGCCTCAGTTAAA
AACTTTACTCAACTTATCGAAAGCCTAGATAAACCAACGGAACAAATTGCAATTGAAGCCCGAATTGTCACTATCAGCAG
TGAACATTTACAACAACTTGGCGTGCGTTGGGGCTTATTTTCCCCTAACGAAAATCACTACAAATTGGCAGGCAATTTAG
AAGGCAACGGATTAACTACCAACAACTTAAACGTAAATTTTCCAGTAAATCCGTCCGCTTCTGTTGCCTTACAAATTGCT
GCAATCAACAGCCGTGTACTTGATTTAGAACTTACTGCATTAGAAAGCGAAAATAACATAGAGATCATTGCAAGCCCTCG
CTTACTCACAACAGATAAAAAAACAGCGAGTATCAAACAAGGTACAGAAATTCCTTATGCAATGTACAGTAAGAAAGAGG
AAATCACCGATATTGAATTTCGTGAAGCCGTTTTGGGGTTAGAGGTCACGCCACATATTTCTAAACAAAATCAGATTTTG
TTAGACCTTGCTATCAGCCAAAATTCGCCCAATAACCAAATAAATAATACAATGGTGACAATTGATAAACAGGAAATCAA
AACGCAAGTTCTAGCTAAACACGGTGAAACCATCGTATTAGGCGGTATTTTTCAACATCTGATCGCCAAAGGCGAAGATA
AAGTACCGCTGTTAGGCAGTATCCCCGTGATTAAACGCTTATTTAGCCAAAGCCAAGATAAAATCTCTAAACGAGAATTA
GTGATTTTTGTCACGCCTTATATTGTGAAATCTGAAAAAATAGGAGCGGAAAAGCAGAAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(253-410)

Bacterial type II/III secretion system short domain (Secretin_N)

(110-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A836Z0A6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

52.941

100

0.536
  comE Haemophilus influenzae 86-028NP

52

100

0.526
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.923

98.571

0.374
  pilQ Vibrio cholerae strain A1552

37.923

98.571

0.374
  pilQ Vibrio campbellii strain DS40M4

36.967

100

0.371

Multiple sequence alignment    



References

[1] Ke Dai et al. (2018) Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401. Frontiers in Cellular And Infection Microbiology 8:32. [PMID: 29473023]