Detailed information    

experimental Experimentally validated

Overview

Name   ssb   Type   Machinery gene
Locus tag   NGFG_RS05740 Genome accession   NC_022240
Coordinates   1059259..1059783 (-) Length   174 a.a.
NCBI ID   WP_003695064.1    Uniprot ID   Q5F7Y0
Organism   Neisseria gonorrhoeae MS11     
Function   ssDNA binding   
DNA processing

Function

To understand the biochemical features of N. gonorrhoeae RecA (RecA(Ng)), we overexpressed and purified the RecA(Ng) and SSB(Ng) proteins and compared their activities to those of the well-characterized E. coli RecA and SSB proteins in vitro.


Genomic Context

Location: 1054259..1064783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS05725 (NGFG_00709) recP/tkt 1054387..1056366 (+) 1980 WP_003688227.1 transketolase Machinery gene
  NGFG_RS05730 (NGFG_00708) fumC 1056510..1057898 (+) 1389 WP_003695070.1 class II fumarate hydratase -
  NGFG_RS05735 (NGFG_00707) - 1057963..1058886 (-) 924 WP_003695068.1 DMT family transporter -
  NGFG_RS05740 (NGFG_00705) ssb 1059259..1059783 (-) 525 WP_003695064.1 single-stranded DNA-binding protein Machinery gene
  NGFG_RS05745 (NGFG_00704) - 1059787..1061169 (-) 1383 WP_003688217.1 MFS transporter -
  NGFG_RS05750 (NGFG_00703) - 1061281..1061904 (-) 624 WP_003688215.1 lytic transglycosylase domain-containing protein -
  NGFG_RS05760 (NGFG_00701) - 1062406..1062672 (-) 267 Protein_1089 transposase -
  NGFG_RS05765 (NGFG_00700) - 1062709..1063199 (-) 491 Protein_1090 REP-associated tyrosine transposase -
  NGFG_RS05770 (NGFG_02337) - 1063561..1064414 (-) 854 Protein_1091 opacity family porin -

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 19448.66 Da        Isoelectric Point: 5.8313

>NTDB_id=1131 NGFG_RS05740 WP_003695064.1 1059259..1059783(-) (ssb) [Neisseria gonorrhoeae MS11]
MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQRVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRI
QSRKYQGKDGIERTAYDIVANEMKMLGGRNENSGGAPYDEGYGQSQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
AAAPVEDIDDDIPF

Nucleotide


Download         Length: 525 bp        

>NTDB_id=1131 NGFG_RS05740 WP_003695064.1 1059259..1059783(-) (ssb) [Neisseria gonorrhoeae MS11]
ATGTCATTGAACAAAGTCATCCTTATCGGCCGCCTCGGACGCGATCCCGAAGTGCGCTATATGCCCAACGGCGAGGCAGT
GTGTAATTTCAGCGTCGCCACCAGCGAAACTTGGAACGACCGCAACGGCCAGCGCGTAGAGCGCACCGAATGGCACAACA
TCACGATGTACCGCAAACTGGCGGAAATTGCCGGGCAATACCTCAAAAAAGGCGGGCTGGTTTATCTGGAAGGCAGAATC
CAAAGCCGCAAATACCAAGGCAAAGACGGCATCGAACGCACCGCTTACGATATTGTCGCCAATGAAATGAAAATGTTGGG
CGGGCGCAATGAAAACAGCGGCGGCGCGCCTTACGATGAAGGTTACGGTCAGAGTCAGGAGGCTTACCAACGCCCCGCTC
AGCAAAGCCGGCAGCCCGCTCCCGACGCGCCGTCCCATCCCCAAGAAGCACCCGCCGCGCCGCGCCGCCAACCCGTGCCG
GCCGCCGCCCCGGTCGAGGACATTGACGACGACATCCCGTTCTGA

Domains

Predicted by InterProScan.

Single-strand binding protein family (SSB)

(3-106)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5F7Y0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Neisseria meningitidis MC58

98.851

100

0.989
  ssb Vibrio cholerae strain A1552

47.778

100

0.494
  ssb Glaesserella parasuis strain SC1401

44.505

100

0.466

Multiple sequence alignment    



References

[1] Elizabeth A Stohl et al. (2011) Purification and characterization of the RecA protein from Neisseria gonorrhoeae. PloS One 6(2):e17101. [PMID: 21359151]