Detailed information    

experimental Experimentally validated

Overview

Name   ssb   Type   Machinery gene
Locus tag   NGFG_RS05740 Genome accession   NC_022240
Coordinates   1059259..1059783 (-) Length   174 a.a.
NCBI ID   WP_003695064.1    Uniprot ID   Q5F7Y0
Organism   Neisseria gonorrhoeae MS11     
Function   ssDNA binding   
DNA processing

Function

To understand the biochemical features of N. gonorrhoeae RecA (RecA(Ng)), we overexpressed and purified the RecA(Ng) and SSB(Ng) proteins and compared their activities to those of the well-characterized E. coli RecA and SSB proteins in vitro.


Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1037540..1059783 1059259..1059783 within 0


Gene organization within MGE regions


Location: 1037540..1059783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS05595 (NGFG_00728) - 1037540..1037908 (-) 369 WP_003688254.1 hypothetical protein -
  NGFG_RS05600 (NGFG_00727) cas7c 1037935..1038695 (-) 761 Protein_1062 type I-C CRISPR-associated protein Cas7/Csd2 -
  NGFG_RS05615 (NGFG_00726) cas8c 1038715..1040001 (-) 1287 Protein_1063 type I-C CRISPR-associated protein Cas8c/Csd1 -
  NGFG_RS13080 - 1039998..1040177 (-) 180 Protein_1064 type I-C CRISPR-associated protein Cas5c -
  NGFG_RS15960 - 1040153..1040673 (-) 521 Protein_1065 IS630 transposase-related protein -
  NGFG_RS05625 (NGFG_00725) - 1040836..1041045 (-) 210 WP_003688249.1 TraR/DksA family transcriptional regulator -
  NGFG_RS05630 (NGFG_00724) - 1041038..1041259 (-) 222 WP_010358352.1 hypothetical protein -
  NGFG_RS05635 (NGFG_00723) - 1041333..1041620 (-) 288 WP_003688246.1 hypothetical protein -
  NGFG_RS13090 (NGFG_00722) - 1041740..1042378 (-) 639 WP_003691011.1 hypothetical protein -
  NGFG_RS15525 - 1042375..1042749 (-) 375 WP_010358353.1 hypothetical protein -
  NGFG_RS05650 (NGFG_00721) - 1042746..1043786 (-) 1041 WP_003691008.1 nucleoid-associated protein -
  NGFG_RS05655 (NGFG_00720) - 1044020..1044400 (-) 381 WP_003688243.1 helix-turn-helix domain-containing protein -
  NGFG_RS05665 (NGFG_00718) - 1044672..1044902 (+) 231 WP_003702555.1 hypothetical protein -
  NGFG_RS05670 (NGFG_00717) - 1044889..1045173 (+) 285 WP_003706365.1 winged helix-turn-helix transcriptional regulator -
  NGFG_RS05675 (NGFG_00716) - 1045210..1046083 (+) 874 Protein_1075 hypothetical protein -
  NGFG_RS05680 (NGFG_00715) - 1046076..1047842 (+) 1767 WP_003695077.1 DDE-type integrase/transposase/recombinase -
  NGFG_RS14430 - 1047883..1048071 (-) 189 WP_071201060.1 hypothetical protein -
  NGFG_RS05685 (NGFG_00714) - 1048181..1049566 (-) 1386 Protein_1078 3'-5' exonuclease -
  NGFG_RS15530 - 1050015..1050131 (+) 117 Protein_1079 IS5/IS1182 family transposase -
  NGFG_RS05700 (NGFG_00713) - 1050289..1051464 (+) 1176 WP_003691002.1 NAD(P)H-dependent flavin oxidoreductase -
  NGFG_RS05705 (NGFG_00712) - 1051806..1052354 (-) 549 WP_003688232.1 FixH family protein -
  NGFG_RS05710 (NGFG_00711) ccoG 1052357..1053853 (-) 1497 WP_003688230.1 cytochrome c oxidase accessory protein CcoG -
  NGFG_RS05725 (NGFG_00709) tkt 1054387..1056366 (+) 1980 WP_003688227.1 transketolase -
  NGFG_RS05730 (NGFG_00708) fumC 1056510..1057898 (+) 1389 WP_003695070.1 class II fumarate hydratase -
  NGFG_RS05735 (NGFG_00707) - 1057963..1058886 (-) 924 WP_003695068.1 DMT family transporter -
  NGFG_RS05740 (NGFG_00705) ssb 1059259..1059783 (-) 525 WP_003695064.1 single-stranded DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 19448.66 Da        Isoelectric Point: 5.8313

>NTDB_id=1131 NGFG_RS05740 WP_003695064.1 1059259..1059783(-) (ssb) [Neisseria gonorrhoeae MS11]
MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQRVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRI
QSRKYQGKDGIERTAYDIVANEMKMLGGRNENSGGAPYDEGYGQSQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
AAAPVEDIDDDIPF

Nucleotide


Download         Length: 525 bp        

>NTDB_id=1131 NGFG_RS05740 WP_003695064.1 1059259..1059783(-) (ssb) [Neisseria gonorrhoeae MS11]
ATGTCATTGAACAAAGTCATCCTTATCGGCCGCCTCGGACGCGATCCCGAAGTGCGCTATATGCCCAACGGCGAGGCAGT
GTGTAATTTCAGCGTCGCCACCAGCGAAACTTGGAACGACCGCAACGGCCAGCGCGTAGAGCGCACCGAATGGCACAACA
TCACGATGTACCGCAAACTGGCGGAAATTGCCGGGCAATACCTCAAAAAAGGCGGGCTGGTTTATCTGGAAGGCAGAATC
CAAAGCCGCAAATACCAAGGCAAAGACGGCATCGAACGCACCGCTTACGATATTGTCGCCAATGAAATGAAAATGTTGGG
CGGGCGCAATGAAAACAGCGGCGGCGCGCCTTACGATGAAGGTTACGGTCAGAGTCAGGAGGCTTACCAACGCCCCGCTC
AGCAAAGCCGGCAGCCCGCTCCCGACGCGCCGTCCCATCCCCAAGAAGCACCCGCCGCGCCGCGCCGCCAACCCGTGCCG
GCCGCCGCCCCGGTCGAGGACATTGACGACGACATCCCGTTCTGA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(3-106)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5F7Y0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Neisseria meningitidis MC58

98.851

100

0.989
  ssb Vibrio cholerae strain A1552

47.778

100

0.494
  ssb Glaesserella parasuis strain SC1401

44.505

100

0.466

Multiple sequence alignment    



References

[1] Elizabeth A Stohl et al. (2011) Purification and characterization of the RecA protein from Neisseria gonorrhoeae. PloS One 6(2):e17101. [PMID: 21359151]