Detailed information    

experimental Experimentally validated

Overview

Name   comC   Type   Machinery gene
Locus tag   LCA_RS08810 Genome accession   NC_007576
Coordinates   1742507..1743196 (+) Length   229 a.a.
NCBI ID   WP_011375453.1    Uniprot ID   Q38UQ6
Organism   Latilactobacillus sakei subsp. sakei 23K     
Function   DNA uptake   
DNA binding and uptake

Function

All the late competence (com) operons encoding structural elements of the DNA uptake machinery were highly activated by sigHLsa overexpression.


Genomic Context

Location: 1737507..1748196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCA_RS08790 (LCA_1766) - 1737948..1738499 (+) 552 WP_011375451.1 cysteine hydrolase family protein -
  LCA_RS08795 (LCA_1768) fusA 1739070..1741157 (-) 2088 WP_011375452.1 elongation factor G -
  LCA_RS08800 (LCA_1769) rpsG 1741253..1741723 (-) 471 WP_004270185.1 30S ribosomal protein S7 -
  LCA_RS08805 (LCA_1770) rpsL 1741813..1742226 (-) 414 WP_004270179.1 30S ribosomal protein S12 -
  LCA_RS08810 (LCA_1771) comC 1742507..1743196 (+) 690 WP_011375453.1 A24 family peptidase Machinery gene
  LCA_RS09965 (LCA_1772) - 1743200..1743484 (-) 285 WP_011375454.1 isochorismatase family protein -
  LCA_RS09840 (LCA_1773) - 1743414..1743773 (-) 360 WP_011375455.1 isochorismatase family protein -
  LCA_RS08820 (LCA_1774) rpoC 1743947..1747591 (-) 3645 WP_011375456.1 DNA-directed RNA polymerase subunit beta' -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sigH comC positive effect
  sigH comEC positive effect
  sigH comFC positive effect
  sigH comGE positive effect
  sigH recA positive effect
  sigH comGA positive effect
  sigH ssb positive effect
  sigH comEA positive effect
  sigH comFA positive effect
  sigH comGB positive effect
  sigH comGC positive effect
  sigH dprA positive effect
  sigH comGD positive effect
  sigH comGF positive effect

Sequence

Protein


Download         Length: 229 a.a.        Molecular weight: 26187.50 Da        Isoelectric Point: 7.4351

>NTDB_id=623 LCA_RS08810 WP_011375453.1 1742507..1743196(+) (comC) [Latilactobacillus sakei subsp. sakei 23K]
MLLYLIIFYSGACCASFLTVCAWRLPIEKSIITPRSHCDCCQQSLAWHDLLPLFSYLYLRGHCRTCHAQIKPTFLFSELI
GGLLACFIFSESLSWDLAYLLVILFYISLVDLFYYILYPIPLFASLVPLFYLYWPQNHWLGAIVFCCGLLLTTYWASGFG
FGDVELLTILTLWVGLESVLRILLAACLICILFQGIKKIWPSPKQATRQIPFIPYISMGVVIILLQTPQ

Nucleotide


Download         Length: 690 bp        

>NTDB_id=623 LCA_RS08810 WP_011375453.1 1742507..1743196(+) (comC) [Latilactobacillus sakei subsp. sakei 23K]
ATGTTACTTTATTTAATCATCTTTTATAGCGGTGCTTGTTGCGCCTCATTTCTAACAGTTTGCGCCTGGCGCTTACCCAT
CGAAAAGTCCATTATCACACCACGTTCGCACTGCGATTGCTGTCAGCAGTCACTGGCTTGGCATGATTTATTACCACTAT
TCAGCTACTTATACTTACGTGGCCACTGCCGCACTTGTCATGCTCAGATTAAGCCCACTTTTTTATTCAGCGAACTAATT
GGTGGTCTATTGGCCTGCTTCATTTTTAGCGAATCCCTTTCTTGGGACCTCGCTTATTTACTCGTGATTCTTTTTTACAT
CAGTCTCGTTGACCTATTCTACTATATTCTCTATCCAATCCCCTTATTCGCATCACTGGTGCCCCTCTTTTATTTGTATT
GGCCACAGAATCATTGGCTAGGAGCCATCGTCTTTTGTTGCGGGCTGTTGCTCACCACTTACTGGGCGTCTGGTTTTGGT
TTTGGCGATGTCGAGTTACTCACTATCCTAACGTTGTGGGTCGGCCTTGAATCCGTTTTGCGAATTCTCTTAGCAGCCTG
TTTAATCTGTATTCTATTTCAAGGCATCAAAAAAATCTGGCCATCGCCTAAACAAGCGACGCGCCAGATTCCTTTTATTC
CCTATATATCGATGGGAGTGGTGATAATACTCCTACAAACACCACAATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(99-193)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(11-89)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q38UQ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Solveig Schmid et al. (2012) Alternative sigma factor σH activates competence gene expression in Lactobacillus sakei. BMC Microbiology 12:32. [PMID: 22409597]