Detailed information    

experimental Experimentally validated

Overview

Name   comEC   Type   Machinery gene
Locus tag   LCA_RS05330 Genome accession   NC_007576
Coordinates   1063849..1066116 (-) Length   755 a.a.
NCBI ID   WP_011374769.1    Uniprot ID   Q38WR1
Organism   Latilactobacillus sakei subsp. sakei 23K     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

All the late competence (com) operons encoding structural elements of the DNA uptake machinery were highly activated by sigHLsa overexpression.


Genomic Context

Location: 1058849..1071116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCA_RS05305 (LCA_1064) - 1058970..1059917 (-) 948 WP_011374764.1 hypothetical protein -
  LCA_RS05310 (LCA_1065) - 1060006..1061724 (-) 1719 WP_011374765.1 ribonuclease J -
  LCA_RS05315 (LCA_1066) rpsO 1061953..1062222 (-) 270 WP_011374766.1 30S ribosomal protein S15 -
  LCA_RS05320 (LCA_1067) rpsT 1062501..1062755 (+) 255 WP_011374767.1 30S ribosomal protein S20 -
  LCA_RS05325 (LCA_1068) holA 1062824..1063852 (-) 1029 WP_011374768.1 DNA polymerase III subunit delta -
  LCA_RS05330 (LCA_1069) comEC 1063849..1066116 (-) 2268 WP_011374769.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LCA_RS05335 (LCA_1070) - 1066113..1066601 (-) 489 WP_011374770.1 ComE operon protein 2 -
  LCA_RS05340 (LCA_1071) comEA 1066659..1067366 (-) 708 WP_231952379.1 helix-hairpin-helix domain-containing protein Machinery gene
  LCA_RS05345 (LCA_1072) - 1067438..1068484 (-) 1047 WP_011374772.1 SepM family pheromone-processing serine protease -
  LCA_RS05350 (LCA_1073) coaD 1068486..1068983 (-) 498 WP_011374773.1 pantetheine-phosphate adenylyltransferase -
  LCA_RS05355 (LCA_1074) rsmD 1068996..1069550 (-) 555 WP_011374774.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  LCA_RS05360 (LCA_1075) - 1069555..1069845 (-) 291 WP_011374775.1 YlbG family protein -
  LCA_RS05365 (LCA_1076) - 1069869..1070957 (-) 1089 WP_011374776.1 CAP-associated domain-containing protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sigH comEC positive effect
  sigH comFC positive effect
  sigH comGE positive effect
  sigH recA positive effect
  sigH comGA positive effect
  sigH ssb positive effect
  sigH comEA positive effect
  sigH comFA positive effect
  sigH comGB positive effect
  sigH comGC positive effect
  sigH dprA positive effect
  sigH comGD positive effect
  sigH comGF positive effect
  sigH comC positive effect

Sequence

Protein


Download         Length: 755 a.a.        Molecular weight: 85357.14 Da        Isoelectric Point: 10.1105

>NTDB_id=614 LCA_RS05330 WP_011374769.1 1063849..1066116(-) (comEC) [Latilactobacillus sakei subsp. sakei 23K]
MKNNWIYPVICLISLNSLMLGGQSWLTWSLLLLAFVKLISLRQTQITIMTIGLCLVYGGRFWISYHSVTQPQQPVAQYFK
VQPDAIQVAGDSVQLTAIGQQDGQTIKAYYRCQTFAEKRRWQQVRHPVLFYGEKDFKRIQGATNQNEFDYARFLAQQKRC
FYQLTLAKKTTFALRRPSSGLDWLHYWRQTCALYLRKLPTALRFHAQTLLLGLREAQVDHYQVVLGHLGIIHLLSLSGLH
VFYLVQVIRWCATYCRIPRERLNCMLFGLLPVYALLVGGTTSISRAIALILSRLLCKQLGIRQSRLDSWSLVLLVNLFWQ
PYLLHSMGGILSYLMAFALIYIGEGSTFKVAYWLSLLSLPVCLRFNYRWHVLTIMMNALVTPIYLPIVLGLAIVASVMLP
VSNLVVNGCEWLLQLIYKGLGLVVQIPHAMITFGKIPLLPLLLIVTVSLLLIDGQAISNQWRKRLKRTLVSLYLASFLAI
HFNPTGRVVMFDIGQGDSLLIQTPFNRHQLLIDTGGRLALPQAAWQRRAQVSRAEKVTVNYLYSQGVDHIDAVALSHQDA
DHIGDLNQILKQIRVDRIICAAGLPQNRQFQRQIRPFLATVQIEPYLAGAAFKVGSQKFNVLAPTKPGKGENSDSLVLQA
QIGGASWLFTGDLEQEGERAIIERYPQLTVDYLKVGHHGSKTASDPTVIKKLHLKGALISTGRENRYGHPHQETLTTLQA
AKVPYWLTAQQGMLTWAYGPGQSEKLQTTIKDSEK

Nucleotide


Download         Length: 2268 bp        

>NTDB_id=614 LCA_RS05330 WP_011374769.1 1063849..1066116(-) (comEC) [Latilactobacillus sakei subsp. sakei 23K]
ATGAAAAACAACTGGATTTATCCAGTTATTTGTTTAATCAGTTTAAATAGCTTAATGCTTGGGGGGCAATCTTGGTTAAC
CTGGAGCTTATTATTATTGGCTTTTGTCAAATTAATAAGTCTCAGACAAACTCAGATAACGATTATGACGATCGGCTTAT
GCCTTGTTTACGGGGGCCGCTTTTGGATAAGCTATCATAGTGTGACGCAACCGCAACAACCGGTAGCACAGTACTTTAAA
GTGCAACCCGATGCGATTCAAGTAGCGGGCGATAGTGTCCAATTAACGGCAATCGGCCAACAAGATGGTCAGACTATTAA
GGCTTATTATCGCTGCCAAACGTTCGCCGAGAAGCGGCGATGGCAGCAAGTACGACACCCCGTTTTGTTCTATGGGGAAA
AAGATTTTAAACGTATACAGGGTGCTACCAATCAAAATGAGTTTGATTATGCCCGTTTTTTAGCGCAACAGAAGCGCTGC
TTTTACCAACTGACGTTAGCCAAAAAAACAACTTTTGCGCTGAGACGCCCTAGTAGCGGTTTGGATTGGCTACATTATTG
GCGCCAAACGTGTGCACTGTATTTACGTAAGTTACCAACAGCACTACGGTTTCATGCGCAGACGTTACTATTAGGTTTGC
GGGAAGCGCAAGTTGACCATTACCAGGTTGTTTTGGGCCACTTAGGGATTATTCATCTCTTAAGTCTATCAGGGTTGCAC
GTGTTTTATTTAGTGCAAGTCATTCGGTGGTGTGCGACTTATTGCCGCATACCGCGTGAGCGGTTAAACTGTATGTTATT
TGGGCTATTGCCGGTATATGCCTTACTGGTTGGTGGTACGACAAGCATTAGTCGCGCGATTGCGCTTATTTTATCCCGGT
TACTTTGCAAACAGTTGGGCATTCGGCAATCAAGGTTAGATAGTTGGAGTTTAGTATTACTGGTCAATTTATTCTGGCAG
CCTTATTTGCTACATAGTATGGGCGGGATTTTAAGTTATTTGATGGCCTTTGCGCTCATTTATATTGGTGAGGGTAGCAC
TTTTAAAGTTGCTTACTGGCTAAGTTTATTGAGTTTGCCGGTTTGCTTACGCTTTAATTATCGCTGGCATGTGCTGACGA
TAATGATGAATGCCTTGGTAACGCCTATCTATCTGCCAATTGTGCTAGGTTTGGCGATTGTAGCGAGTGTGATGTTACCT
GTTAGCAATCTAGTGGTTAACGGATGTGAGTGGCTGTTACAGTTGATTTACAAAGGCTTAGGACTAGTTGTTCAAATTCC
CCATGCGATGATTACGTTTGGTAAAATACCATTATTACCGTTACTATTGATTGTGACGGTCAGTCTCTTGTTAATCGATG
GCCAGGCAATTAGTAATCAATGGCGTAAACGGCTGAAACGCACATTGGTAAGTTTGTATCTGGCTAGTTTTTTGGCGATT
CATTTTAATCCGACTGGTCGGGTGGTCATGTTTGATATTGGTCAAGGTGATAGTCTGTTGATTCAGACGCCGTTTAACCG
GCATCAGCTTTTAATCGATACTGGTGGTCGCCTAGCGCTACCGCAAGCAGCTTGGCAACGGCGGGCGCAGGTTTCACGAG
CGGAAAAAGTAACGGTTAATTATCTCTATAGCCAAGGAGTAGATCACATTGATGCGGTGGCACTTTCTCATCAGGATGCC
GATCATATTGGTGATTTGAACCAGATTTTAAAACAAATTCGTGTTGATCGAATCATTTGTGCTGCCGGATTACCGCAAAA
TCGGCAATTTCAAAGACAAATTCGACCATTTTTAGCAACGGTTCAGATAGAACCCTATTTAGCCGGGGCCGCTTTTAAAG
TGGGATCACAAAAATTTAACGTCTTAGCACCGACTAAGCCGGGAAAAGGCGAAAATAGTGATTCGTTAGTCTTACAGGCA
CAAATTGGTGGGGCTAGCTGGCTTTTTACAGGAGATCTAGAACAAGAAGGTGAACGGGCAATTATCGAGCGGTATCCACA
ATTAACCGTCGATTATTTAAAAGTTGGCCATCATGGTAGTAAAACGGCTAGTGACCCCACCGTGATTAAAAAATTGCACT
TAAAAGGCGCATTAATCTCAACCGGCAGAGAGAATCGTTATGGTCATCCGCACCAGGAAACTTTGACGACTTTGCAAGCG
GCCAAAGTGCCTTATTGGCTAACGGCGCAGCAGGGGATGTTGACCTGGGCGTACGGTCCCGGTCAAAGCGAAAAACTGCA
AACAACAATAAAGGATAGTGAAAAATGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(209-452)

Metallo-beta-lactamase superfamily (Lactamase_B)

(494-702)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q38WR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Solveig Schmid et al. (2012) Alternative sigma factor σH activates competence gene expression in Lactobacillus sakei. BMC Microbiology 12:32. [PMID: 22409597]