Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   LCA_RS04960 Genome accession   NC_007576
Coordinates   985501..986367 (-) Length   288 a.a.
NCBI ID   WP_011374694.1    Uniprot ID   -
Organism   Latilactobacillus sakei subsp. sakei 23K     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

Whereas recA was little activated, expression of dprA was highly induced in the sigH(hy)* context.


Genomic Context

Location: 980501..991367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCA_RS04945 (LCA_0989) topA 981736..983820 (-) 2085 WP_011374691.1 type I DNA topoisomerase -
  LCA_RS04950 (LCA_0990) - 984071..984505 (-) 435 WP_011374692.1 Rrf2 family transcriptional regulator -
  LCA_RS04955 (LCA_0991) - 984534..985400 (-) 867 WP_011374693.1 NAD(P)H-binding protein -
  LCA_RS04960 (LCA_0992) dprA 985501..986367 (-) 867 WP_011374694.1 DNA-processing protein DprA Machinery gene
  LCA_RS04965 (LCA_0993) - 986422..987183 (-) 762 WP_011374695.1 ribonuclease HII -
  LCA_RS04970 (LCA_0994) ylqF 987185..988036 (-) 852 WP_167524711.1 ribosome biogenesis GTPase YlqF -
  LCA_RS04975 (LCA_0995) lepB 988168..988698 (-) 531 WP_011374697.1 signal peptidase I -
  LCA_RS04980 (LCA_0996) - 988723..988947 (-) 225 WP_011374698.1 YozE family protein -
  LCA_RS04985 (LCA_0997) - 988958..989587 (-) 630 WP_011374699.1 YpmS family protein -
  LCA_RS04990 (LCA_0998) - 989600..990502 (-) 903 WP_011374700.1 SGNH/GDSL hydrolase family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sigH dprA positive effect
  sigH comEC positive effect
  sigH comFC positive effect
  sigH comGE positive effect
  sigH recA positive effect
  sigH comGA positive effect
  sigH ssb positive effect
  sigH comEA positive effect
  sigH comFA positive effect
  sigH comGB positive effect
  sigH comGC positive effect
  sigH comGD positive effect
  sigH comGF positive effect
  sigH comC positive effect

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31821.78 Da        Isoelectric Point: 8.8338

>NTDB_id=626 LCA_RS04960 WP_011374694.1 985501..986367(-) (dprA) [Latilactobacillus sakei subsp. sakei 23K]
MLTKNQLLLKVHLAAGFGTISELRLAAWLSTSHNWALSALEIAQIARLPERYWPTFQASFQSTTLQRQCIDHEVRTTYLT
ILDKDYPQRLLETYLPPVLLFYRGDLRLLKQPCLAVVGARQATHYSKQSLEQLLQGLTATTIISGLAQGADAMAHEVALQ
RGLAPIGVIGTGINCSYPPQNEHLQQVVAEKGLLLSEYALGTPARRYQFPARNKIIAGLCHSLIVTEARHKSGSLITANL
ALQANRNVYAIPGRIDQSLSQGCNQLIAAGATPLLDKNILIEELRYFD

Nucleotide


Download         Length: 867 bp        

>NTDB_id=626 LCA_RS04960 WP_011374694.1 985501..986367(-) (dprA) [Latilactobacillus sakei subsp. sakei 23K]
GTGTTAACTAAAAATCAATTACTCTTAAAAGTACATTTAGCGGCGGGTTTTGGGACTATTAGTGAATTACGCTTGGCGGC
GTGGCTATCAACCAGTCATAATTGGGCGTTATCGGCGTTAGAAATTGCGCAGATTGCGCGCTTGCCGGAACGTTATTGGC
CGACTTTTCAAGCAAGTTTTCAAAGTACAACCTTACAACGCCAATGCATCGACCATGAAGTTCGAACGACTTATTTAACC
ATCTTAGATAAAGATTACCCACAACGCTTATTGGAAACCTATTTGCCGCCCGTTTTGTTATTTTATCGTGGTGATTTAAG
ATTGTTAAAACAGCCATGTCTCGCGGTAGTCGGGGCGCGTCAAGCGACTCATTATTCTAAACAATCACTAGAACAGTTAT
TACAAGGACTAACTGCCACTACGATTATTAGTGGGTTAGCACAAGGAGCGGATGCAATGGCCCACGAAGTTGCCTTACAG
CGTGGTTTGGCCCCAATTGGGGTGATTGGCACGGGCATTAATTGCAGTTATCCACCACAAAATGAGCATTTACAACAGGT
AGTTGCTGAAAAAGGTTTGTTATTATCTGAATACGCTCTTGGTACACCAGCCCGGCGCTATCAATTTCCAGCCCGCAATA
AAATCATCGCGGGGTTATGTCATAGTTTAATTGTGACGGAAGCCCGGCACAAGAGTGGTAGCTTGATTACCGCTAACTTG
GCCCTTCAAGCTAATCGCAATGTCTATGCAATTCCGGGGCGCATTGATCAATCACTGTCACAGGGGTGCAACCAATTAAT
CGCCGCGGGAGCGACACCATTGTTAGATAAAAACATATTAATAGAAGAACTACGTTATTTTGATTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Solveig Schmid et al. (2012) Alternative sigma factor σH activates competence gene expression in Lactobacillus sakei. BMC Microbiology 12:32. [PMID: 22409597]