Detailed information NTG0000663

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: STER_RS00390
• Genome accession: NC_008532
• Coordinates: 64678..65817 (+)
• Length: 379 a.a.
• NCBI ID: WP_011680606.1
• Uniprot ID: Q03MX5
• Organism: Streptococcus thermophilus LMD-9
• Function: homologous recombination Homologous recombination

Function

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.

Genomic Context

Location: 59678..70817

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
STER_RS00365 (STER_0072)	hexB	59678..61621 (+)	1944	WP_011680601.1	DNA mismatch repair endonuclease MutL	Machinery gene
STER_RS00370 (STER_0073)	-	61751..61972 (+)	222	WP_011680602.1	hypothetical protein	-
STER_RS00375 (STER_0074)	ruvA	62124..62714 (+)	591	WP_011680603.1	Holliday junction branch migration protein RuvA	Machinery gene
STER_RS00380 (STER_0075)	-	62721..63275 (+)	555	WP_011680604.1	DNA-3-methyladenine glycosylase I	-
STER_RS00385 (STER_0076)	cinA	63371..64642 (+)	1272	WP_011680605.1	competence/damage-inducible protein A	Machinery gene
STER_RS00390 (STER_0077)	recA	64678..65817 (+)	1140	WP_011680606.1	recombinase RecA	Machinery gene
STER_RS00395 (STER_0078)	spx	65994..66392 (+)	399	WP_002949058.1	transcriptional regulator Spx	-

Sequence

Protein

• Length: 379 a.a.
• Molecular weight: 40911.49 Da
• Isoelectric Point: 4.8394

>NTDB_id=663 STER_RS00390 WP_011680606.1 64678..65817(+) (recA) [Streptococcus thermophilus LMD-9]
MAKKTKKTEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGERAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGP
ESSGKTTVALHAVAQTQKEGGIAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGIMFGNPETTPGGRALKFYASVRLDVRG
NTQIKGTGDKKDQNVGKETKIKVVKNKVAPPFKEAFVEIMYGEGISQTGELVKIASDIGIIQKAGAWFSYNGEKIGQGSE
NAKKYLADHPEIFAEIDHKVRVHYGLIELDEDDVVEDTQVEDTSDELILDLDSTIEIEE

Nucleotide

• Length: 1140 bp

>NTDB_id=663 STER_RS00390 WP_011680606.1 64678..65817(+) (recA) [Streptococcus thermophilus LMD-9]
GTGGCTAAGAAAACAAAGAAAACAGAAGAAATCACAAAGAAGTTTGGTGATGAGCGTCGCAAAGCACTCGACGATGCATT
AAAAAACATTGAAAAAGATTTTGGTAAGGGTGCAGTTATGCGTCTTGGTGAGCGTGCAGAGCAAAAAGTTCAGGTTATGA
GCTCAGGCTCACTAGCTTTGGATATTGCTCTTGGTGCGGGTGGTTACCCTAAAGGTCGTATTATTGAAATTTACGGACCA
GAATCATCAGGTAAAACAACTGTTGCCCTTCATGCAGTTGCTCAGACTCAAAAAGAAGGTGGCATCGCAGCTTTTATCGA
TGCCGAGCATGCTCTTGACCCTGCGTATGCAGCAGCTCTAGGTGTTAATATCGATGAGCTTCTTTTGTCGCAGCCTGATT
CTGGTGAGCAAGGTCTCGAAATTGCAGGTAAGCTGATTGACTCTGGTGCAGTGGATTTAGTTGTTGTTGACTCAGTTGCG
GCCTTGGTACCACGTGCAGAAATTGATGGAGATATTGGTGACAGTCATGTAGGACTTCAAGCGCGTATGATGAGTCAAGC
CATGCGTAAACTTTCTGCATCTATTAATAAAACAAAAACGATTGCTATCTTTATTAACCAGTTGCGTGAAAAAGTTGGTA
TCATGTTTGGTAACCCAGAGACTACCCCAGGTGGACGTGCTTTAAAATTCTATGCATCAGTACGTCTTGATGTACGTGGT
AATACACAAATTAAAGGAACCGGTGACAAAAAGGACCAAAATGTTGGTAAGGAAACCAAGATTAAGGTTGTTAAAAACAA
AGTTGCTCCACCATTTAAAGAAGCTTTTGTTGAAATTATGTATGGCGAAGGAATTTCACAAACCGGTGAACTTGTAAAAA
TTGCAAGTGATATAGGCATTATTCAGAAAGCCGGAGCTTGGTTCTCATATAATGGGGAGAAAATTGGTCAAGGATCTGAA
AATGCTAAAAAGTATTTAGCAGATCACCCTGAGATTTTTGCAGAAATCGATCATAAAGTACGCGTACACTACGGTCTGAT
TGAGCTAGATGAGGACGATGTTGTTGAAGATACACAAGTTGAAGACACGTCTGATGAACTCATTCTAGATCTTGATTCAA
CCATTGAAATCGAAGAATAA

Domains

Predicted by InterProScan.

• recA bacterial DNA recombination protein (RecA_N) (21-286)

3D structure

• AlphaFold DB: Q03MX5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Streptococcus thermophilus LMG 18311	98.438	100	0.997
recA	Streptococcus pyogenes NZ131	89.182	100	0.894
recA	Streptococcus mutans UA159	88.251	100	0.892
recA	Streptococcus mitis SK321	86.352	100	0.868
recA	Streptococcus mitis NCTC 12261	86.352	100	0.868
recA	Streptococcus pneumoniae R36A	84.935	100	0.863
recA	Streptococcus pneumoniae Rx1	84.935	100	0.863
recA	Streptococcus pneumoniae D39	84.935	100	0.863
recA	Streptococcus pneumoniae R6	84.935	100	0.863
recA	Streptococcus pneumoniae TIGR4	84.935	100	0.863
recA	Lactococcus lactis subsp. cremoris KW2	77.62	93.14	0.723
recA	Latilactobacillus sakei subsp. sakei 23K	66.575	100	0.679
recA	Bacillus subtilis subsp. subtilis str. 168	66	100	0.664
recA	Staphylococcus aureus strain ATCC 12600	64.47	100	0.648
recA	Acinetobacter nosocomialis M2	58.824	100	0.6
recA	Neisseria gonorrhoeae strain FA1090	59.827	99.425	0.595
recA	Neisseria gonorrhoeae MS11	59.827	99.425	0.595
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	60.177	98.834	0.595
recA	Acinetobacter baylyi ADP1	62.154	93.123	0.579
recA	Acinetobacter baumannii D1279779	62.154	93.123	0.579
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	56.891	99.417	0.566
recA	Vibrio cholerae O1 biovar El Tor strain E7946	61.231	91.808	0.562
recA	Vibrio cholerae strain A1552	61.231	91.808	0.562
recA	Ralstonia pseudosolanacearum GMI1000	57.31	97.159	0.557
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	62.539	88.981	0.556
recA	Pseudomonas stutzeri DSM 10701	58.462	93.66	0.548
recA	Glaesserella parasuis strain SC1401	59.412	91.398	0.543
recA	Helicobacter pylori strain NCTC11637	56.667	95.101	0.539
recA	Helicobacter pylori 26695	56.667	95.101	0.539

References

• [1]: Gabriele Giliberti et al. (2006) Transcriptional analysis of the recA gene of Streptococcus thermophilus. Microbial Cell Factories 5:29. [PMID: 16972988]
• [2]: Gabriele Giliberti et al. (2002) Alteration of cell morphology and viability in a recA mutant of Streptococcus thermophilus upon induction of heat shock and nutrient starvation. Gene 295(1):1-6. [PMID: 12242004]