Detailed information NTG0000664

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: STU_RS09850
• Genome accession: NC_006448
• Coordinates: 64654..65808 (+)
• Length: 384 a.a.
• NCBI ID: WP_002949056.1
• Uniprot ID: Q5M6H2
• Organism: Streptococcus thermophilus LMG 18311
• Function: homologous recombination Homologous recombination

Function

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.

Genomic Context

Location: 59654..70808

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
STU_RS09825 (stu0054)	hexB	59654..61597 (+)	1944	WP_011225282.1	DNA mismatch repair endonuclease MutL	Machinery gene
STU_RS09830 (stu0055)	-	61727..61948 (+)	222	WP_041826940.1	hypothetical protein	-
STU_RS09835 (stu0056)	ruvA	62100..62690 (+)	591	WP_002949053.1	Holliday junction branch migration protein RuvA	Machinery gene
STU_RS09840 (stu0057)	-	62697..63251 (+)	555	WP_011225284.1	DNA-3-methyladenine glycosylase I	-
STU_RS09845 (stu0059)	cinA	63347..64618 (+)	1272	WP_011225285.1	competence/damage-inducible protein A	Machinery gene
STU_RS09850 (stu0060)	recA	64654..65808 (+)	1155	WP_002949056.1	recombinase RecA	Machinery gene
STU_RS09855 (stu0061)	spx	65985..66383 (+)	399	WP_002949058.1	transcriptional regulator Spx	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 41470.03 Da
• Isoelectric Point: 4.7444

>NTDB_id=664 STU_RS09850 WP_002949056.1 64654..65808(+) (recA) [Streptococcus thermophilus LMG 18311]
MAKKTKKTEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGERAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGP
ESSGKTTVALHAVAQTQKEGGIAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGIMFGNPETTPGGRALKFYASVRLDVRG
NTQIKGTGDKKDQNVGKETKIKVVKNKVAPPFKEAFVEIMYGEGISQTGELVKIASDIGIIQKAGAWFSYNGEKIGQGSE
NAKKYLADHPEIFAEIDHKVRVHYGLVELDEDDVVEDTQVEDTQVEDTSDELILDLDSTIEIEE

Nucleotide

• Length: 1155 bp

>NTDB_id=664 STU_RS09850 WP_002949056.1 64654..65808(+) (recA) [Streptococcus thermophilus LMG 18311]
GTGGCTAAGAAAACAAAGAAAACAGAAGAAATCACAAAGAAGTTTGGTGATGAGCGTCGCAAAGCACTCGACGATGCATT
AAAAAACATTGAAAAAGATTTTGGTAAGGGTGCAGTTATGCGTCTTGGTGAGCGTGCAGAGCAAAAAGTTCAGGTTATGA
GCTCAGGCTCGCTAGCTTTGGATATTGCTCTTGGTGCGGGTGGTTACCCTAAAGGTCGTATTATTGAAATTTACGGACCA
GAATCATCAGGTAAAACAACTGTTGCCCTTCATGCAGTTGCTCAGACTCAAAAAGAAGGTGGCATCGCAGCTTTTATCGA
TGCCGAGCATGCTCTTGACCCTGCGTATGCAGCAGCTCTAGGTGTTAATATCGATGAGCTTCTTTTGTCGCAGCCTGATT
CTGGTGAGCAAGGTCTCGAAATTGCAGGTAAGCTGATTGACTCTGGTGCAGTGGATTTAGTTGTTGTTGACTCAGTTGCG
GCCTTGGTACCACGTGCAGAAATTGATGGAGATATTGGTGACAGTCATGTAGGACTTCAAGCGCGTATGATGAGTCAAGC
CATGCGTAAACTTTCTGCATCTATTAATAAAACAAAAACGATTGCTATCTTTATTAACCAGTTGCGTGAAAAAGTTGGTA
TCATGTTTGGTAACCCAGAGACTACCCCAGGTGGACGTGCTTTAAAATTCTATGCATCAGTACGTCTTGATGTACGTGGT
AATACACAAATTAAAGGAACCGGTGACAAAAAGGACCAAAATGTTGGTAAGGAAACCAAGATTAAGGTTGTTAAAAACAA
AGTTGCTCCACCATTTAAAGAAGCTTTTGTTGAAATTATGTATGGCGAAGGAATTTCACAAACCGGTGAACTTGTAAAAA
TTGCAAGTGATATAGGCATTATTCAGAAAGCCGGAGCTTGGTTCTCATATAATGGGGAGAAAATTGGTCAAGGATCTGAA
AATGCTAAAAAGTATTTAGCAGATCACCCTGAGATTTTTGCAGAAATCGATCATAAAGTACGCGTACACTACGGTCTGGT
TGAGCTAGATGAGGACGATGTTGTTGAAGATACACAAGTTGAAGACACACAAGTTGAAGACACGTCTGATGAACTCATTC
TAGATCTTGATTCAACCATTGAAATCGAAGAATAA

Domains

Predicted by InterProScan.

• recA bacterial DNA recombination protein (RecA_N) (21-286)

3D structure

• AlphaFold DB: Q5M6H2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Streptococcus thermophilus LMD-9	98.438	100	0.997
recA	Streptococcus pyogenes NZ131	88.021	100	0.894
recA	Streptococcus mutans UA159	88.052	100	0.885
recA	Streptococcus mitis NCTC 12261	85.64	99.74	0.854
recA	Streptococcus mitis SK321	85.64	99.74	0.854
recA	Streptococcus pneumoniae Rx1	84.715	100	0.852
recA	Streptococcus pneumoniae R36A	84.715	100	0.852
recA	Streptococcus pneumoniae D39	84.715	100	0.852
recA	Streptococcus pneumoniae R6	84.715	100	0.852
recA	Streptococcus pneumoniae TIGR4	84.715	100	0.852
recA	Lactococcus lactis subsp. cremoris KW2	77.62	91.927	0.714
recA	Bacillus subtilis subsp. subtilis str. 168	66.189	100	0.664
recA	Latilactobacillus sakei subsp. sakei 23K	67.919	97.465	0.662
recA	Staphylococcus aureus strain ATCC 12600	65.306	98.847	0.646
recA	Neisseria gonorrhoeae strain FA1090	59.827	99.425	0.595
recA	Neisseria gonorrhoeae MS11	59.827	99.425	0.595
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	60.177	98.834	0.595
recA	Acinetobacter nosocomialis M2	58.689	100	0.589
recA	Acinetobacter baylyi ADP1	62.154	93.123	0.579
recA	Acinetobacter baumannii D1279779	62.154	93.123	0.579
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	56.598	99.417	0.563
recA	Vibrio cholerae O1 biovar El Tor strain E7946	61.231	91.808	0.562
recA	Vibrio cholerae strain A1552	61.231	91.808	0.562
recA	Ralstonia pseudosolanacearum GMI1000	57.31	97.159	0.557
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	62.539	88.981	0.556
recA	Pseudomonas stutzeri DSM 10701	58.462	93.66	0.548
recA	Glaesserella parasuis strain SC1401	59.412	91.398	0.543
recA	Helicobacter pylori 26695	56.667	95.101	0.539
recA	Helicobacter pylori strain NCTC11637	56.667	95.101	0.539

References

• [1]: Gabriele Giliberti et al. (2006) Transcriptional analysis of the recA gene of Streptococcus thermophilus. Microbial Cell Factories 5:29. [PMID: 16972988]
• [2]: Gabriele Giliberti et al. (2002) Alteration of cell morphology and viability in a recA mutant of Streptococcus thermophilus upon induction of heat shock and nutrient starvation. Gene 295(1):1-6. [PMID: 12242004]