Detailed information NTG0000527

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: SMSK321_RS11065
• Genome accession: NZ_AEDT01000034
• Coordinates: 4759..5913 (+)
• Length: 384 a.a.
• NCBI ID: WP_001085454.1
• Uniprot ID: -
• Organism: Streptococcus mitis SK321
• Function: homologous recombination Homologous recombination

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 1..10913

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMSK321_RS11035 (SMSK321_1652)	ndk	67..489 (+)	423	WP_000438301.1	nucleoside-diphosphate kinase	-
SMSK321_RS11040 (SMSK321_1653)	comM	622..1251 (+)	630	WP_000413917.1	hypothetical protein	Regulator
SMSK321_RS11045 (SMSK321_1654)	tsaE	1348..1791 (+)	444	WP_000288250.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
SMSK321_RS11050 (SMSK321_1655)	-	1781..2299 (+)	519	WP_000455546.1	GNAT family N-acetyltransferase	-
SMSK321_RS11055 (SMSK321_1656)	brpA	2307..3359 (+)	1053	WP_000239273.1	biofilm formation/cell division transcriptional regulator BrpA	-
SMSK321_RS11060 (SMSK321_1657)	cinA	3448..4704 (+)	1257	WP_000642686.1	competence/damage-inducible protein A	Machinery gene
SMSK321_RS11065 (SMSK321_1658)	recA	4759..5913 (+)	1155	WP_001085454.1	recombinase RecA	Machinery gene
SMSK321_RS11070 (SMSK321_1659)	-	6028..7398 (+)	1371	WP_001036240.1	MATE family efflux transporter	-
SMSK321_RS11075 (SMSK321_1660)	-	7495..8211 (+)	717	WP_000532883.1	YebC/PmpR family DNA-binding transcriptional regulator	-
SMSK321_RS11080 (SMSK321_1661)	-	8388..8858 (+)	471	WP_000360539.1	MarR family winged helix-turn-helix transcriptional regulator	-
SMSK321_RS11085 (SMSK321_1662)	rcrP	8833..10602 (+)	1770	WP_000731785.1	ABC transporter ATP-binding protein	Regulator

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 41244.96 Da
• Isoelectric Point: 4.7992

>NTDB_id=527 SMSK321_RS11065 WP_001085454.1 4759..5913(+) (recA) [Streptococcus mitis SK321]
MAKKPKKLDEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGERAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGP
ESSGKTTVALHAVAQAQKEGGIAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGNPETTPGGRALKFYASVRLDVRG
STQIKGTGDQKDTNVGKETKIKVVKNKVAPPFKEAFVEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSE
NAKKYLADNPEVFAEIDHQVRVQFGLIDGEEAAESKKDEVAQVDSVNEEVTLDLGDELEIEIEE

Nucleotide

• Length: 1155 bp

>NTDB_id=527 SMSK321_RS11065 WP_001085454.1 4759..5913(+) (recA) [Streptococcus mitis SK321]
ATGGCGAAAAAACCAAAAAAATTAGATGAAATTTCAAAAAAATTCGGGGCAGAACGTGAAAAAGCCTTGAATGACGCTCT
TAAATTGATTGAGAAAGACTTTGGTAAGGGTTCAATCATGCGTTTGGGTGAACGTGCTGAGCAAAAGGTGCAAGTGATGA
GCTCCGGTTCCTTGGCTCTTGATATTGCCCTTGGTTCAGGTGGTTATCCTAAGGGACGTATCATCGAAATCTACGGACCA
GAGTCATCTGGTAAGACAACGGTTGCTCTTCATGCAGTTGCGCAAGCGCAAAAAGAAGGTGGAATTGCAGCCTTTATCGA
TGCGGAACATGCCCTCGATCCAGCTTATGCTGCGGCCCTTGGTGTCAACATTGACGAATTGCTCTTGTCTCAACCAGATT
CAGGAGAGCAAGGTCTTGAGATTGCAGGAAAATTGATTGACTCAGGTGCTGTAGATCTTGTCGTGGTCGACTCAGTTGCT
GCCCTTGTACCTCGTGCAGAAATTGATGGAGATATCGGAGATAGCCACGTTGGTTTGCAGGCTCGTATGATGAGCCAGGC
TATGCGTAAACTTGGTGCTTCTATCAATAAAACCAAAACAATTGCCATCTTTATCAACCAATTGCGTGAAAAAGTTGGGG
TCATGTTTGGAAATCCAGAAACAACTCCTGGTGGACGTGCTCTGAAATTCTACGCATCAGTCCGTTTGGATGTTCGTGGA
AGCACACAAATCAAGGGAACTGGTGACCAAAAAGATACCAATGTCGGTAAAGAAACTAAAATCAAGGTCGTGAAAAACAA
GGTAGCTCCACCATTTAAGGAAGCCTTCGTTGAAATCATGTACGGAGAAGGGATTTCTAAGACTGGTGAACTCTTAAAAA
TCGCAAGCGATTTGGATATCATCAAAAAAGCAGGAGCTTGGTACTCTTACAAGGATGAGAAAATCGGGCAAGGTTCTGAA
AATGCCAAGAAATACTTGGCAGATAACCCAGAAGTCTTTGCTGAGATTGATCATCAAGTCCGTGTTCAATTTGGTTTGAT
TGATGGAGAAGAAGCTGCTGAAAGCAAAAAAGATGAAGTAGCTCAAGTAGACTCTGTGAATGAAGAAGTAACTCTTGATC
TAGGCGATGAGCTTGAAATCGAAATTGAAGAATAA

Domains

Predicted by InterproScan.

• recA bacterial DNA recombination protein (RecA) (21-286)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Streptococcus mitis NCTC 12261	98.958	100	0.99
recA	Streptococcus pneumoniae TIGR4	93.557	100	0.945
recA	Streptococcus pneumoniae Rx1	93.557	100	0.945
recA	Streptococcus pneumoniae D39	93.557	100	0.945
recA	Streptococcus pneumoniae R6	93.557	100	0.945
recA	Streptococcus pyogenes NZ131	85.039	100	0.857
recA	Streptococcus mutans UA159	83.77	99.739	0.836
recA	Lactococcus lactis subsp. cremoris KW2	73.834	99.742	0.736
recA	Latilactobacillus sakei subsp. sakei 23K	68.222	96.62	0.659
recA	Bacillus subtilis subsp. subtilis str. 168	65.994	99.713	0.658
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	62.195	95.627	0.595
recA	Acinetobacter baumannii D1279779	60.411	97.708	0.59
recA	Neisseria gonorrhoeae strain FA1090	60.606	94.828	0.575
recA	Neisseria gonorrhoeae MS11	60.606	94.828	0.575
recA	Neisseria gonorrhoeae MS11	60.606	94.828	0.575
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	64.396	88.981	0.573
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	57.647	99.125	0.571
recA	Acinetobacter baylyi ADP1	61.043	93.41	0.57
recA	Vibrio cholerae O1 biovar El Tor strain E7946	61.92	91.243	0.565
recA	Vibrio cholerae strain A1552	61.92	91.243	0.565
recA	Pseudomonas stutzeri DSM 10701	56.069	99.712	0.559
recA	Ralstonia pseudosolanacearum GMI1000	59.394	93.75	0.557
recA	Glaesserella parasuis strain SC1401	60.588	91.398	0.554
recA	Helicobacter pylori 26695	57.447	94.813	0.545
recA	Helicobacter pylori strain NCTC11637	57.447	94.813	0.545

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]