Detailed information    

experimental Experimentally validated

Overview

Name   cinA   Type   Machinery gene
Locus tag   SMSK321_RS11060 Genome accession   NZ_AEDT01000034
Coordinates   3448..4704 (+) Length   418 a.a.
NCBI ID   WP_000642686.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   facilitate localization of RecA   
Homologous recombination

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 1..9704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS11035 (SMSK321_1652) ndk 67..489 (+) 423 WP_000438301.1 nucleoside-diphosphate kinase -
  SMSK321_RS11040 (SMSK321_1653) comM 622..1251 (+) 630 WP_000413917.1 hypothetical protein Regulator
  SMSK321_RS11045 (SMSK321_1654) tsaE 1348..1791 (+) 444 WP_000288250.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
  SMSK321_RS11050 (SMSK321_1655) - 1781..2299 (+) 519 WP_000455546.1 GNAT family N-acetyltransferase -
  SMSK321_RS11055 (SMSK321_1656) brpA 2307..3359 (+) 1053 WP_000239273.1 biofilm formation/cell division transcriptional regulator BrpA -
  SMSK321_RS11060 (SMSK321_1657) cinA 3448..4704 (+) 1257 WP_000642686.1 competence/damage-inducible protein A Machinery gene
  SMSK321_RS11065 (SMSK321_1658) recA 4759..5913 (+) 1155 WP_001085454.1 recombinase RecA Machinery gene
  SMSK321_RS11070 (SMSK321_1659) - 6028..7398 (+) 1371 WP_001036240.1 MATE family efflux transporter -
  SMSK321_RS11075 (SMSK321_1660) - 7495..8211 (+) 717 WP_000532883.1 YebC/PmpR family DNA-binding transcriptional regulator -
  SMSK321_RS11080 (SMSK321_1661) - 8388..8858 (+) 471 WP_000360539.1 MarR family winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 44989.28 Da        Isoelectric Point: 4.6144

>NTDB_id=538 SMSK321_RS11060 WP_000642686.1 3448..4704(+) (cinA) [Streptococcus mitis SK321]
MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGDNEARLLSLLEIASQRSSLVILTGGLGPTEDDLTKQT
LAKFLGKELVFDPQAQEKLDVFFAQRPDYARTPNNERQAQIVEGATPLPNETGLAVGGILEVDGVTYVVLPGPPSELKPM
VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILSDLIDNQTDPTLAPYAKTGEVTLRLSTKASSQEEANQVLDILENQI
LGRQTFEGLSLRDLCYGYGEEASLASIVVEELKKQGKTITAAESLTAGLFQATVADFSGASSIFKGGFVTYSLEEKSKML
DIPVKDLEEQGVVSEFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPAGTVFIGLAQEHGTKVIKVNIGGRSRADVR
HIAVMHAFNLVRKALLSD

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=538 SMSK321_RS11060 WP_000642686.1 3448..4704(+) (cinA) [Streptococcus mitis SK321]
ATGAAAGCGGAAATCATTGCTGTTGGAACAGAGATTTTGACCGGACAGATTGTTAATACCAATGCCCAGTTTTTGTCGGA
AAAACTAGCAGAGATTGGGGTAGACGTATATTTTCAGACGGCTGTAGGAGACAATGAAGCTCGTCTCTTGTCTTTGCTTG
AAATTGCCAGTCAACGTAGCAGTCTGGTGATTTTGACAGGTGGTTTAGGGCCAACTGAGGACGATTTGACCAAACAAACT
CTGGCTAAATTTTTAGGGAAAGAATTAGTCTTCGATCCTCAGGCTCAGGAGAAGTTGGATGTCTTTTTTGCCCAGAGACC
AGACTATGCCCGAACACCGAATAACGAAAGACAAGCTCAAATTGTAGAAGGAGCGACTCCACTGCCCAACGAAACTGGAC
TGGCTGTGGGAGGCATTTTGGAAGTCGATGGAGTGACCTACGTTGTCCTTCCAGGTCCACCAAGTGAATTGAAACCGATG
GTCTTAAACCAACTTCTACCCAAGTTGATGACAGGGAGCAAGCTGTATTCCCGAGTTCTTCGTTTCTTTGGAATTGGCGA
GAGCCAGCTGGTTACGATTTTGTCTGATTTGATTGATAATCAAACAGATCCGACCTTGGCCCCTTATGCTAAGACAGGGG
AGGTAACCTTGCGTCTGTCAACAAAGGCTAGCAGTCAAGAAGAGGCGAATCAAGTGCTGGATATCTTGGAAAATCAAATC
TTAGGTCGCCAGACCTTTGAAGGCCTTTCTTTACGAGACCTCTGTTATGGTTATGGAGAGGAGGCTAGCTTGGCCAGCAT
TGTGGTAGAAGAACTGAAAAAACAAGGGAAAACTATCACGGCTGCAGAGAGTTTGACGGCAGGGCTTTTCCAAGCTACTG
TGGCGGATTTTTCAGGAGCTTCAAGTATATTTAAGGGTGGTTTCGTGACCTATAGCCTGGAGGAAAAATCAAAGATGTTG
GATATTCCTGTCAAGGACTTGGAAGAACAGGGTGTGGTATCTGAATTTACAGCTCAGAAGATGGCTGAGCAGGCACGAAG
CAAGACCCAGTCAGATTTTGGTATTAGTTTGACTGGAGTGGCAGGACCAGATAGCCTCGAAGGACACCCGGCTGGAACAG
TCTTCATAGGCTTGGCACAGGAGCATGGAACTAAGGTCATCAAGGTTAATATTGGAGGTAGAAGTCGAGCAGATGTACGT
CACATTGCGGTTATGCATGCCTTTAACCTAGTTCGCAAGGCTTTATTAAGTGACTAA

Domains

Predicted by InterproScan.

Probable molybdopterin binding domain (MoCF_biosynth)

(5-169)

Damage-inducible protein CinA KH domain (CinA_KH)

(177-240)

Competence-damaged protein (CinA)

(262-414)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mitis NCTC 12261

97.368

100

0.974
  cinA Streptococcus pneumoniae TIGR4

93.541

100

0.935
  cinA Streptococcus pneumoniae R6

93.301

100

0.933
  cinA Streptococcus pneumoniae Rx1

93.301

100

0.933
  cinA Streptococcus pneumoniae D39

93.062

100

0.931
  cinA Streptococcus mutans UA159

72.488

100

0.725
  cinA Streptococcus suis isolate S10

54.808

100

0.58
  cinA Bacillus subtilis subsp. subtilis str. 168

45.694

100

0.459

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]