Detailed information    

experimental Experimentally validated

Overview

Name   cinA   Type   Machinery gene
Locus tag   SPD_RS09270 Genome accession   NC_008533
Coordinates   1733832..1735088 (-) Length   418 a.a.
NCBI ID   WP_000642701.1    Uniprot ID   Q04IM5
Organism   Streptococcus pneumoniae D39     
Function   facilitate localization of RecA   
Homologous recombination

Function

CinA has been reported to play an important role during competence of Streptococcus pneumoniae. A protein–protein interaction between CinA and RecA was observed in vitro, and the localization of RecA to the membrane of competent S. pneumoniae cells was shown to be dependent on CinA.


Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1726515..1748922 1733832..1735088 within 0


Gene organization within MGE regions


Location: 1726515..1748922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPD_RS09220 (SPD_1733) - 1726515..1726712 (-) 198 WP_000890144.1 hypothetical protein -
  SPD_RS09225 (SPD_1734) - 1726716..1727510 (-) 795 WP_001101210.1 hypothetical protein -
  SPD_RS09230 (SPD_1735) - 1727918..1728343 (-) 426 WP_000204065.1 hypothetical protein -
  SPD_RS09235 (SPD_1736) - 1728472..1728831 (-) 360 WP_000749822.1 ImmA/IrrE family metallo-endopeptidase -
  SPD_RS09240 - 1728877..1729083 (-) 207 WP_000366091.1 hypothetical protein -
  SPD_RS09245 - 1729070..1729243 (-) 174 WP_000289349.1 helix-turn-helix domain-containing protein -
  SPD_RS09250 (SPD_1737) lytA 1729602..1730558 (-) 957 WP_000405235.1 N-acetylmuramoyl-L-alanine amidase LytA Auxiliary factor
  SPD_RS09255 (SPD_1738) - 1730934..1732304 (-) 1371 WP_001036271.1 MATE family efflux transporter -
  SPD_RS09265 (SPD_1739) recA 1732611..1733777 (-) 1167 WP_001085462.1 recombinase RecA Machinery gene
  SPD_RS09270 (SPD_1740) cinA 1733832..1735088 (-) 1257 WP_000642701.1 competence/damage-inducible protein A Machinery gene
  SPD_RS09275 (SPD_1741) brpA 1735173..1736189 (-) 1017 WP_000239282.1 biofilm formation/cell division transcriptional regulator BrpA -
  SPD_RS09280 (SPD_1742) - 1736197..1736715 (-) 519 WP_000455537.1 GNAT family N-acetyltransferase -
  SPD_RS09285 (SPD_1743) tsaE 1736705..1737148 (-) 444 WP_000288232.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
  SPD_RS09290 (SPD_1744) comM 1737234..1737854 (-) 621 WP_000839908.1 hypothetical protein Regulator
  SPD_RS09300 (SPD_1745) - 1738237..1739100 (-) 864 WP_001093641.1 helix-turn-helix domain-containing protein -
  SPD_RS11945 (SPD_1746) - 1739292..1739462 (+) 171 WP_001227862.1 PhrA family quorum-sensing system peptide -
  SPD_RS09310 (SPD_1747) pneA1 1739565..1739789 (+) 225 WP_000181087.1 two-peptide bacteriocin subunit PneA1 -
  SPD_RS09315 (SPD_1748) pneA2 1739796..1739984 (+) 189 WP_000786759.1 two-peptide bacteriocin subunit PneA2 -
  SPD_RS09320 (SPD_1749) - 1740100..1743090 (+) 2991 WP_000875575.1 type 2 lanthipeptide synthetase LanM family protein -
  SPD_RS09325 (SPD_1750) - 1743440..1744144 (+) 705 WP_001033170.1 flavodoxin family protein -
  SPD_RS09330 (SPD_1751) - 1744125..1745054 (+) 930 WP_000912955.1 M50 family metallopeptidase -
  SPD_RS09335 (SPD_1752) - 1745051..1747159 (+) 2109 WP_000701298.1 peptidase domain-containing ABC transporter -
  SPD_RS09340 (SPD_1753) - 1747183..1748922 (+) 1740 WP_000699969.1 S8 family peptidase -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45064.41 Da        Isoelectric Point: 4.5211

>NTDB_id=239 SPD_RS09270 WP_000642701.1 1733832..1735088(-) (cinA) [Streptococcus pneumoniae D39]
MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGDNEVRLLSLLEIASQRSSLVILTGGLGATEDDLTKQT
LAKFLGKALVFDPQAQEKLDIFFALRPDYARTPNNERQVQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLRLSTKASSQEEANQALDILENQI
LDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQGKTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRML
DIPAKNLEEHGVVSEFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIGGRSRADVR
HIAVMHAFNLVRKALLSD

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=239 SPD_RS09270 WP_000642701.1 1733832..1735088(-) (cinA) [Streptococcus pneumoniae D39]
ATGAAAGCAGAAATCATTGCTGTTGGAACAGAGATTTTGACAGGACAGATTGTCAACACCAATGCCCAGTTTTTGTCGGA
AAAACTAGCTGAGATTGGGGTAGATGTATATTTTCAGACGGCTGTAGGAGACAATGAAGTTCGTCTCTTGTCTTTGCTTG
AGATTGCCAGTCAACGTAGCAGTCTGGTGATTTTGACAGGCGGTTTGGGGGCAACTGAGGACGACCTAACCAAACAAACC
CTAGCTAAATTTTTAGGGAAAGCATTAGTCTTTGATCCTCAGGCTCAGGAGAAGTTGGATATCTTTTTTGCCCTGCGACC
AGACTATGCCCGAACACCGAATAACGAAAGACAAGTTCAAATTGTAGAAGGAGCGATTCCACTGCCAAACGAAACAGGAC
TGGCTGTTGGAGGAAAATTAGAAGTAGACGGAGTGACCTATGTCGTCCTTCCAGGTCCGCCAAGTGAATTGAAACCCATG
GTCTTAAACCAACTTCTACCCAAGTTGATGACAGGGAGCAAGCTGTATTCCCGAGTTCTTCGTTTCTTTGGGATTGGCGA
GAGCCAGTTGGTTACGATTTTGGCTGATTTAATTGATAATCAGATCGATCCTACCTTGGCCCCTTATGCCAAGACAGGAG
AAGTCACTCTACGTCTGTCAACAAAGGCTAGCAGTCAAGAAGAGGCGAATCAAGCGCTGGATATCTTGGAAAATCAAATC
TTGGACTGCCAGACTTTCGAAGGAATTTCTTTACGAGACTTTTGCTATGGTTATGGGGAAGAAACTAGTTTAGCCAGCAT
TGTGGTAGAAGAACTGAAAAGGCAAGGGAAAACCATCGCGGCTGCAGAGAGTTTGACGGCAGGTCTTTTCCAAGCTACCG
TGGCGAATTTTTCTGGAGTTTCAAGTATATTTGAGGGTGGTTTTGTGACCTATAGCTTGGAGGAAAAATCAAGGATGTTG
GATATTCCTGCCAAGAATTTGGAAGAACATGGTGTGGTGTCTGAATTTACAGCTCAGAAGATGGCTGAGCAGGCACGAAG
CAAGACCCAGTCTGATTTTGGAATTAGTTTGACTGGAGTGGCAGGACCAGATAGCCTAGAAGGACACCCAGTTGGGACAG
TCTTCATAGGCTTGGCGCAAGATCAAGGAACTGAGGTTATCAAGGTTAATATTGGAGGCAGAAGCCGAGCAGATGTACGT
CACATTGCGGTTATGCATGCCTTTAACCTAGTTCGCAAGGCTTTATTAAGTGACTAA

Domains

Predicted by InterproScan.

Damage-inducible protein CinA KH domain (CinA_KH)

(177-240)

Probable molybdopterin binding domain (MoCF_biosynth)

(5-169)

Competence-damaged protein (CinA)

(262-414)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q04IM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus pneumoniae R6

99.761

100

0.998
  cinA Streptococcus pneumoniae Rx1

99.761

100

0.998
  cinA Streptococcus pneumoniae TIGR4

99.043

100

0.99
  cinA Streptococcus mitis SK321

93.062

100

0.931
  cinA Streptococcus mitis NCTC 12261

92.823

100

0.928
  cinA Streptococcus mutans UA159

70.335

100

0.703
  cinA Streptococcus suis isolate S10

54.567

100

0.578
  cinA Bacillus subtilis subsp. subtilis str. 168

44.976

100

0.452

Multiple sequence alignment    



References

[1] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
[2] Ping Luo et al. (2003) Transient association of an alternative sigma factor, ComX, with RNA polymerase during the period of competence for genetic transformation in Streptococcus pneumoniae. Journal of Bacteriology 185(1):349-58. [PMID: 12486073]
[3] M S Lee et al. (1999) Identification of a new regulator in Streptococcus pneumoniae linking quorum sensing to competence for genetic transformation. Journal of Bacteriology 181(16):5004-16. [PMID: 10438773]
[4] I Mortier-Barrière et al. (1998) Competence-specific induction of recA is required for full recombination proficiency during transformation in Streptococcus pneumoniae. Molecular Microbiology 27(1):159-70. [PMID: 9466264]
[5] H R Masure et al. (1998) Membrane targeting of RecA during genetic transformation. Molecular Microbiology 27(4):845-52. [PMID: 9515709]