Detailed information    

experimental Experimentally validated

Overview

Name   cinA   Type   Machinery gene
Locus tag   BSU_16930 Genome accession   NC_000964
Coordinates   1763222..1764472 (+) Length   416 a.a.
NCBI ID   NP_389575.2    Uniprot ID   P46323
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   unknown   
Unclear

Function

The cinA gene encodes a protein that localizes to the nucleoid and plays a minor role in competent cells.


Genomic Context

Location: 1758222..1769472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_16860 (BSU16860) ymfH 1758314..1759600 (+) 1287 NP_389568.2 putative processing protease -
  BSU_16870 (BSU16870) efpI 1759655..1760383 (+) 729 NP_389569.2 EF-P-5 aminopentanone reductase (EF-P repair enzyme), NADPH-dependent -
  BSU_16880 (BSU16880) ymfJ 1760464..1760721 (+) 258 NP_389570.1 putative enzyme -
  BSU_16910 (BSU16910) rodZ 1761707..1762573 (+) 867 NP_389573.3 cell shape determination factor -
  BSU_16920 (BSU16920) pgsA 1762623..1763204 (+) 582 NP_389574.2 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase -
  BSU_16930 (BSU16930) cinA 1763222..1764472 (+) 1251 NP_389575.2 competence-damage inducible regulator Machinery gene
  BSU_16940 (BSU16940) recA 1764645..1765691 (+) 1047 NP_389576.2 multifunctional SOS repair factor Machinery gene
  BSU_16950 (BSU16950) pbpX 1765859..1767034 (+) 1176 NP_389577.1 penicillin-binding endopeptidase X (lysozyme resistance) -
  BSU_16960 (BSU16960) rny 1767310..1768872 (+) 1563 NP_389578.1 endoribonuclease Y -

Sequence

Protein


Download         Length: 416 a.a.        Molecular weight: 45537.85 Da        Isoelectric Point: 5.3737

>NTDB_id=124 BSU_16930 NP_389575.2 1763222..1764472(+) (cinA) [Bacillus subtilis subsp. subtilis str. 168]
MEFPKKAEIIAVGSELLLGQIANTNAQFISKQLAEIGVNVFYHTAVGDNPERLKQVIRIAEERSDFIIFSGGLGPTKDDL
TKETIANTLGRPLVLNDEAFQSIEDYFKRTKRTMSPNNRKQALVIEGSDVLANHFGMAPGMLTEHESRYYMLLPGPPSEL
RPMFENEAKPLLLKKMGSNEKIVSTVLRFFGIGESQLEADLEDIIDAQTNPTIAPLAADGEVTLRLTAKHADEKETERLL
KETEAVILERVGEFFYGYDDTSLVKELSIACKEKGITISAAESFTGGLFSEWLTDHSGASKLFAGGVVCYTNDVKQNVLG
VKKETLDRFGAVSKECASELAKGVQKLTGSDIGISFTGVAGPDAQEGHEPGHVFIGISANGKEEVHEFHFAGSRTGIRKR
GAKYGCHLILKLLEQK

Nucleotide


Download         Length: 1251 bp        

>NTDB_id=124 BSU_16930 NP_389575.2 1763222..1764472(+) (cinA) [Bacillus subtilis subsp. subtilis str. 168]
ATGGAATTTCCAAAAAAAGCAGAAATAATTGCAGTCGGTTCAGAGTTGCTGCTTGGTCAAATCGCCAATACAAATGCTCA
ATTTATCAGCAAACAGCTCGCTGAAATCGGGGTCAACGTATTTTATCATACAGCGGTCGGAGATAACCCGGAGCGGCTGA
AGCAGGTCATCCGCATTGCTGAAGAACGCTCTGACTTCATTATTTTTTCAGGAGGGCTTGGACCCACAAAAGATGATCTG
ACGAAAGAAACGATTGCAAATACGCTGGGACGTCCGCTTGTGTTAAATGATGAGGCATTCCAATCCATTGAGGACTATTT
CAAACGAACAAAACGCACGATGTCACCTAATAACCGAAAACAGGCGCTTGTGATCGAAGGCTCTGACGTGCTGGCGAATC
ACTTTGGAATGGCCCCGGGAATGCTCACAGAGCATGAATCGCGCTATTATATGCTTCTTCCTGGACCGCCGAGCGAATTG
CGTCCAATGTTTGAAAACGAGGCAAAGCCTCTTCTGCTGAAAAAGATGGGTTCAAATGAAAAAATTGTGTCAACTGTTCT
TCGCTTTTTCGGTATCGGTGAATCTCAGCTTGAAGCCGATTTGGAAGATATTATTGATGCACAAACCAATCCGACAATTG
CGCCTTTGGCGGCTGATGGAGAGGTGACGCTGCGTCTGACAGCCAAACACGCCGATGAAAAGGAAACAGAGCGTCTGTTA
AAAGAAACAGAGGCCGTTATCTTAGAGCGTGTCGGTGAATTTTTCTATGGTTACGATGATACTTCTCTTGTAAAAGAGCT
ATCTATAGCATGTAAGGAAAAAGGCATAACAATTTCTGCGGCAGAAAGCTTTACCGGAGGGCTGTTTTCTGAATGGCTGA
CGGATCATAGCGGTGCCTCAAAATTATTTGCCGGGGGCGTTGTTTGCTATACAAACGACGTGAAGCAGAATGTGCTTGGT
GTCAAGAAGGAAACATTAGATCGTTTTGGGGCGGTCAGCAAGGAGTGCGCATCCGAACTGGCTAAGGGTGTTCAAAAACT
CACTGGCAGCGATATCGGCATTAGCTTTACTGGTGTAGCAGGACCTGATGCTCAAGAAGGGCATGAGCCTGGGCATGTGT
TTATCGGCATTTCCGCAAATGGTAAAGAAGAGGTTCACGAGTTTCACTTTGCGGGCTCCAGAACGGGGATCAGAAAACGC
GGCGCTAAATACGGCTGCCATTTAATCTTAAAGCTTTTAGAGCAAAAATAA

Domains

Predicted by InterproScan.

Competence-damaged protein (CinA)

(262-412)

Probable molybdopterin binding domain (MoCF_biosynth)

(9-172)

Damage-inducible protein CinA KH domain (CinA_KH)

(184-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P46323

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mitis SK321

45.694

100

0.459
  cinA Streptococcus mitis NCTC 12261

45.694

100

0.459
  cinA Streptococcus pneumoniae TIGR4

45.455

100

0.457
  cinA Streptococcus mutans UA159

46.005

99.279

0.457
  cinA Streptococcus pneumoniae Rx1

45.215

100

0.454
  cinA Streptococcus pneumoniae R6

45.215

100

0.454
  cinA Streptococcus pneumoniae D39

44.976

100

0.452
  cinA Streptococcus suis isolate S10

41.379

95.929

0.397

Multiple sequence alignment    



References

[1] Christine Kaimer et al. (2010) Bacillus subtilis CinA is a stationary phase-induced protein that localizes to the nucleoid and plays a minor role in competent cells. Archives of Microbiology 192(7):549-57. [PMID: 20480359]