Detailed information    

experimental Experimentally validated

Overview

Name   cinA   Type   Machinery gene
Locus tag   SPR_RS08830 Genome accession   NC_003098
Coordinates   1726299..1727555 (-) Length   418 a.a.
NCBI ID   WP_000642700.1    Uniprot ID   A0A2U3RWX3
Organism   Streptococcus pneumoniae R6     
Function   facilitate localization of RecA   
Homologous recombination

Function

CinA has been reported to play an important role during competence of Streptococcus pneumoniae. A protein–protein interaction between CinA and RecA was observed in vitro, and the localization of RecA to the membrane of competent S. pneumoniae cells was shown to be dependent on CinA.


Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1718982..1741389 1726299..1727555 within 0


Gene organization within MGE regions


Location: 1718982..1741389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS08785 (spr1748) - 1718982..1719179 (-) 198 WP_000890144.1 hypothetical protein -
  SPR_RS08790 (spr1749) - 1719183..1719977 (-) 795 WP_001101210.1 hypothetical protein -
  SPR_RS08795 (spr1750) - 1720385..1720810 (-) 426 WP_000204065.1 hypothetical protein -
  SPR_RS08800 (spr1751) - 1720939..1721298 (-) 360 WP_000749822.1 ImmA/IrrE family metallo-endopeptidase -
  SPR_RS08805 (spr1752) - 1721344..1721550 (-) 207 WP_000366091.1 hypothetical protein -
  SPR_RS08810 (spr1753) - 1721537..1721710 (-) 174 WP_000289349.1 helix-turn-helix domain-containing protein -
  SPR_RS08815 (spr1754) lytA 1722069..1723025 (-) 957 WP_000405235.1 N-acetylmuramoyl-L-alanine amidase LytA Auxiliary factor
  SPR_RS08820 (spr1756) - 1723401..1724771 (-) 1371 WP_001036271.1 MATE family efflux transporter -
  SPR_RS08825 (spr1757) recA 1725078..1726244 (-) 1167 WP_001085462.1 recombinase RecA Machinery gene
  SPR_RS08830 (spr1758) cinA 1726299..1727555 (-) 1257 WP_000642700.1 competence/damage-inducible protein A Machinery gene
  SPR_RS08835 (spr1759) brpA 1727640..1728656 (-) 1017 WP_000239282.1 biofilm formation/cell division transcriptional regulator BrpA -
  SPR_RS08840 (spr1760) - 1728664..1729182 (-) 519 WP_000455537.1 GNAT family N-acetyltransferase -
  SPR_RS08845 (spr1761) tsaE 1729172..1729615 (-) 444 WP_000288232.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
  SPR_RS08850 (spr1762) comM 1729701..1730321 (-) 621 WP_000839908.1 hypothetical protein Regulator
  SPR_RS08855 (spr1763) - 1730704..1731567 (-) 864 WP_001093641.1 helix-turn-helix domain-containing protein -
  SPR_RS11015 (spr1764) - 1731759..1731929 (+) 171 WP_001227862.1 PhrA family quorum-sensing system peptide -
  SPR_RS08860 (spr1765) pneA1 1732032..1732256 (+) 225 WP_000181087.1 two-peptide bacteriocin subunit PneA1 -
  SPR_RS08865 (spr1766) pneA2 1732263..1732451 (+) 189 WP_000786759.1 two-peptide bacteriocin subunit PneA2 -
  SPR_RS08870 (spr1767) - 1732567..1735557 (+) 2991 WP_000875575.1 type 2 lanthipeptide synthetase LanM family protein -
  SPR_RS08875 (spr1768) - 1735907..1736611 (+) 705 WP_001033170.1 flavodoxin family protein -
  SPR_RS08880 (spr1769) - 1736592..1737521 (+) 930 WP_000912954.1 M50 family metallopeptidase -
  SPR_RS08885 (spr1770) - 1737518..1739626 (+) 2109 WP_000701298.1 peptidase domain-containing ABC transporter -
  SPR_RS08890 (spr1771) - 1739650..1741389 (+) 1740 WP_000699969.1 S8 family peptidase -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45036.36 Da        Isoelectric Point: 4.5211

>NTDB_id=205 SPR_RS08830 WP_000642700.1 1726299..1727555(-) (cinA) [Streptococcus pneumoniae R6]
MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGDNEVRLLSLLEIASQRSSLVILTGGLGATEDDLTKQT
LAKFLGKALVFDPQAQEKLDIFFALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLRLSTKASSQEEANQALDILENQI
LDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQGKTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRML
DIPAKNLEEHGVVSEFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIGGRSRADVR
HIAVMHAFNLVRKALLSD

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=205 SPR_RS08830 WP_000642700.1 1726299..1727555(-) (cinA) [Streptococcus pneumoniae R6]
ATGAAAGCAGAAATCATTGCTGTTGGAACAGAGATTTTGACAGGACAGATTGTCAACACCAATGCCCAGTTTTTGTCGGA
AAAACTAGCTGAGATTGGGGTAGATGTATATTTTCAGACGGCTGTAGGAGACAATGAAGTTCGTCTCTTGTCTTTGCTTG
AGATTGCCAGTCAACGTAGCAGTCTGGTGATTTTGACAGGCGGTTTGGGGGCAACTGAGGACGACCTAACCAAACAAACC
CTAGCTAAATTTTTAGGGAAAGCATTAGTCTTTGATCCTCAGGCTCAGGAGAAGTTGGATATCTTTTTTGCCCTGCGACC
AGACTATGCCCGAACACCGAATAACGAAAGACAAGCTCAAATTGTAGAAGGAGCGATTCCACTGCCAAACGAAACAGGAC
TGGCTGTTGGAGGAAAATTAGAAGTAGACGGAGTGACCTATGTCGTCCTTCCAGGTCCGCCAAGTGAATTGAAACCCATG
GTCTTAAACCAACTTCTACCCAAGTTGATGACAGGGAGCAAGCTGTATTCCCGAGTTCTTCGTTTCTTTGGGATTGGCGA
GAGCCAGTTGGTTACGATTTTGGCTGATTTAATTGATAATCAGATCGATCCTACCTTGGCCCCTTATGCCAAGACAGGAG
AAGTCACTCTACGTCTGTCAACAAAGGCTAGCAGTCAAGAAGAGGCGAATCAAGCGCTGGATATCTTGGAAAATCAAATC
TTGGACTGCCAGACTTTCGAAGGAATTTCTTTACGAGACTTTTGCTATGGTTATGGGGAAGAAACTAGTTTAGCCAGCAT
TGTGGTAGAAGAACTGAAAAGGCAAGGGAAAACCATCGCGGCTGCAGAGAGTTTGACGGCAGGTCTTTTCCAAGCTACCG
TGGCGAATTTTTCTGGAGTTTCAAGTATATTTGAGGGTGGTTTTGTGACCTATAGCTTGGAGGAAAAATCAAGGATGTTG
GATATTCCTGCCAAGAATTTGGAAGAACATGGTGTGGTGTCTGAATTTACAGCTCAGAAGATGGCTGAGCAGGCACGAAG
CAAGACCCAGTCTGATTTTGGAATTAGTTTGACTGGAGTGGCAGGACCAGATAGCCTAGAAGGACACCCAGTTGGGACAG
TCTTCATAGGCTTGGCGCAAGATCAAGGAACTGAGGTTATCAAGGTTAATATTGGAGGCAGAAGCCGAGCAGATGTACGT
CACATTGCGGTTATGCATGCCTTTAACCTAGTTCGCAAGGCTTTATTAAGTGACTAA

Domains

Predicted by InterproScan.

Damage-inducible protein CinA KH domain (CinA_KH)

(177-240)

Probable molybdopterin binding domain (MoCF_biosynth)

(5-169)

Competence-damaged protein (CinA)

(262-414)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U3RWX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus pneumoniae Rx1

100

100

1
  cinA Streptococcus pneumoniae D39

99.761

100

0.998
  cinA Streptococcus pneumoniae TIGR4

99.282

100

0.993
  cinA Streptococcus mitis SK321

93.301

100

0.933
  cinA Streptococcus mitis NCTC 12261

93.062

100

0.931
  cinA Streptococcus mutans UA159

70.574

100

0.706
  cinA Streptococcus suis isolate S10

54.808

100

0.58
  cinA Bacillus subtilis subsp. subtilis str. 168

45.215

100

0.454

Multiple sequence alignment    



References

[1] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
[2] Ping Luo et al. (2003) Transient association of an alternative sigma factor, ComX, with RNA polymerase during the period of competence for genetic transformation in Streptococcus pneumoniae. Journal of Bacteriology 185(1):349-58. [PMID: 12486073]
[3] M S Lee et al. (1999) Identification of a new regulator in Streptococcus pneumoniae linking quorum sensing to competence for genetic transformation. Journal of Bacteriology 181(16):5004-16. [PMID: 10438773]
[4] I Mortier-Barrière et al. (1998) Competence-specific induction of recA is required for full recombination proficiency during transformation in Streptococcus pneumoniae. Molecular Microbiology 27(1):159-70. [PMID: 9466264]
[5] H R Masure et al. (1998) Membrane targeting of RecA during genetic transformation. Molecular Microbiology 27(4):845-52. [PMID: 9515709]