Detailed information NTG0000649

Experimentally validated

Overview

• Name: cinA
• Type: Machinery gene
• Locus tag: KZB15_RS08685
• Genome accession: NZ_CP079922
• Coordinates: 1727671..1728927 (-)
• Length: 418 a.a.
• NCBI ID: WP_000642700.1
• Uniprot ID: Q7ZAK3
• Organism: Streptococcus pneumoniae R36A
• Function: facilitate localization of RecA Homologous recombination

Function

CinA has been reported to play an important role during competence of Streptococcus pneumoniae. A protein–protein interaction between CinA and RecA was observed in vitro, and the localization of RecA to the membrane of competent S. pneumoniae cells was shown to be dependent on CinA.

Genomic Context

Location: 1722671..1733927

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KZB15_RS08660 (KZB15_08665)	-	1722716..1722922 (-)	207	WP_000366091.1	hypothetical protein	-
KZB15_RS08665 (KZB15_08670)	-	1722909..1723082 (-)	174	WP_000289349.1	helix-turn-helix domain-containing protein	-
KZB15_RS08670 (KZB15_08675)	lytA	1723441..1724397 (-)	957	WP_000405235.1	N-acetylmuramoyl-L-alanine amidase LytA	-
KZB15_RS08675 (KZB15_08680)	-	1724773..1726143 (-)	1371	WP_001036271.1	MATE family efflux transporter	-
KZB15_RS08680 (KZB15_08685)	recA	1726450..1727616 (-)	1167	WP_001085462.1	recombinase RecA	Machinery gene
KZB15_RS08685 (KZB15_08690)	cinA	1727671..1728927 (-)	1257	WP_000642700.1	competence/damage-inducible protein A	Machinery gene
KZB15_RS08690 (KZB15_08695)	-	1729012..1730028 (-)	1017	WP_000239282.1	LCP family protein	-
KZB15_RS08695 (KZB15_08700)	-	1730036..1730554 (-)	519	WP_000455537.1	GNAT family N-acetyltransferase	-
KZB15_RS08700 (KZB15_08705)	tsaE	1730544..1730987 (-)	444	WP_000288232.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
KZB15_RS08705 (KZB15_08710)	comM	1731073..1731693 (-)	621	WP_000839908.1	hypothetical protein	Regulator
KZB15_RS08710 (KZB15_08715)	-	1732076..1732939 (-)	864	WP_001093641.1	helix-turn-helix domain-containing protein	-
KZB15_RS10705	-	1733131..1733301 (+)	171	WP_001227862.1	PhrA family quorum-sensing system peptide	-
KZB15_RS08715 (KZB15_08720)	pneA1	1733404..1733628 (+)	225	WP_000181087.1	two-peptide bacteriocin subunit PneA1	-
KZB15_RS08720 (KZB15_08725)	pneA2	1733635..1733823 (+)	189	WP_000786759.1	two-peptide bacteriocin subunit PneA2	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 45036.36 Da
• Isoelectric Point: 4.5211

>NTDB_id=649 KZB15_RS08685 WP_000642700.1 1727671..1728927(-) (cinA) [Streptococcus pneumoniae R36A]
MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGDNEVRLLSLLEIASQRSSLVILTGGLGATEDDLTKQT
LAKFLGKALVFDPQAQEKLDIFFALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLRLSTKASSQEEANQALDILENQI
LDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQGKTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRML
DIPAKNLEEHGVVSEFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIGGRSRADVR
HIAVMHAFNLVRKALLSD

Nucleotide

• Length: 1257 bp

>NTDB_id=649 KZB15_RS08685 WP_000642700.1 1727671..1728927(-) (cinA) [Streptococcus pneumoniae R36A]
ATGAAAGCAGAAATCATTGCTGTTGGAACAGAGATTTTGACAGGACAGATTGTCAACACCAATGCCCAGTTTTTGTCGGA
AAAACTAGCTGAGATTGGGGTAGATGTATATTTTCAGACGGCTGTAGGAGACAATGAAGTTCGTCTCTTGTCTTTGCTTG
AGATTGCCAGTCAACGTAGCAGTCTGGTGATTTTGACAGGCGGTTTGGGGGCAACTGAGGACGACCTAACCAAACAAACC
CTAGCTAAATTTTTAGGGAAAGCATTAGTCTTTGATCCTCAGGCTCAGGAGAAGTTGGATATCTTTTTTGCCCTGCGACC
AGACTATGCCCGAACACCGAATAACGAAAGACAAGCTCAAATTGTAGAAGGAGCGATTCCACTGCCAAACGAAACAGGAC
TGGCTGTTGGAGGAAAATTAGAAGTAGACGGAGTGACCTATGTCGTCCTTCCAGGTCCGCCAAGTGAATTGAAACCCATG
GTCTTAAACCAACTTCTACCCAAGTTGATGACAGGGAGCAAGCTGTATTCCCGAGTTCTTCGTTTCTTTGGGATTGGCGA
GAGCCAGTTGGTTACGATTTTGGCTGATTTAATTGATAATCAGATCGATCCTACCTTGGCCCCTTATGCCAAGACAGGAG
AAGTCACTCTACGTCTGTCAACAAAGGCTAGCAGTCAAGAAGAGGCGAATCAAGCGCTGGATATCTTGGAAAATCAAATC
TTGGACTGCCAGACTTTCGAAGGAATTTCTTTACGAGACTTTTGCTATGGTTATGGGGAAGAAACTAGTTTAGCCAGCAT
TGTGGTAGAAGAACTGAAAAGGCAAGGGAAAACCATCGCGGCTGCAGAGAGTTTGACGGCAGGTCTTTTCCAAGCTACCG
TGGCGAATTTTTCTGGAGTTTCAAGTATATTTGAGGGTGGTTTTGTGACCTATAGCTTGGAGGAAAAATCAAGGATGTTG
GATATTCCTGCCAAGAATTTGGAAGAACATGGTGTGGTGTCTGAATTTACAGCTCAGAAGATGGCTGAGCAGGCACGAAG
CAAGACCCAGTCTGATTTTGGAATTAGTTTGACTGGAGTGGCAGGACCAGATAGCCTAGAAGGACACCCAGTTGGGACAG
TCTTCATAGGCTTGGCGCAAGATCAAGGAACTGAGGTTATCAAGGTTAATATTGGAGGCAGAAGCCGAGCAGATGTACGT
CACATTGCGGTTATGCATGCCTTTAACCTAGTTCGCAAGGCTTTATTAAGTGACTAA

Domains

Predicted by InterProScan.

• Damage-inducible protein CinA KH domain (CinA_KH) (177-240)

• Probable molybdopterin binding domain (MoCF_biosynth) (5-169)

• Competence-damaged protein (CinA) (262-414)

3D structure

• AlphaFold DB: Q7ZAK3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
cinA	Streptococcus pneumoniae Rx1	100	100	1
cinA	Streptococcus pneumoniae R6	100	100	1
cinA	Streptococcus pneumoniae D39	99.761	100	0.998
cinA	Streptococcus pneumoniae TIGR4	99.282	100	0.993
cinA	Streptococcus mitis SK321	93.301	100	0.933
cinA	Streptococcus mitis NCTC 12261	93.062	100	0.931
cinA	Streptococcus mutans UA159	70.574	100	0.706
cinA	Streptococcus suis isolate S10	54.808	100	0.58
cinA	Bacillus subtilis subsp. subtilis str. 168	45.215	100	0.454

References

• [1]: Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
• [2]: Ping Luo et al. (2003) Transient association of an alternative sigma factor, ComX, with RNA polymerase during the period of competence for genetic transformation in Streptococcus pneumoniae. Journal of Bacteriology 185(1):349-58. [PMID: 12486073]
• [3]: M S Lee et al. (1999) Identification of a new regulator in Streptococcus pneumoniae linking quorum sensing to competence for genetic transformation. Journal of Bacteriology 181(16):5004-16. [PMID: 10438773]
• [4]: I Mortier-Barrière et al. (1998) Competence-specific induction of recA is required for full recombination proficiency during transformation in Streptococcus pneumoniae. Molecular Microbiology 27(1):159-70. [PMID: 9466264]
• [5]: H R Masure et al. (1998) Membrane targeting of RecA during genetic transformation. Molecular Microbiology 27(4):845-52. [PMID: 9515709]
• [6]: B J Pearce et al. (1995) The rec locus, a competence-induced operon in Streptococcus pneumoniae. Journal of Bacteriology 177(1):86-93. [PMID: 7798154]