Detailed information    

experimental Experimentally validated

Overview

Name   cinA   Type   Machinery gene
Locus tag   SM12261_RS08320 Genome accession   NZ_CP028414
Coordinates   1647903..1649159 (-) Length   418 a.a.
NCBI ID   WP_000642690.1    Uniprot ID   A0AAX2L6F9
Organism   Streptococcus mitis NCTC 12261     
Function   facilitate localization of RecA   
Homologous recombination

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 1642903..1654159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS08295 (SM12261_1600) - 1643025..1643486 (-) 462 WP_000360529.1 MarR family winged helix-turn-helix transcriptional regulator -
  SM12261_RS08300 (SM12261_1601) - 1643700..1644345 (+) 646 Protein_1508 DUF4190 domain-containing protein -
  SM12261_RS08305 (SM12261_1602) - 1644395..1645111 (-) 717 WP_000532882.1 YebC/PmpR family DNA-binding transcriptional regulator -
  SM12261_RS08310 (SM12261_1603) - 1645208..1646578 (-) 1371 WP_001036250.1 MATE family efflux transporter -
  SM12261_RS08315 (SM12261_1604) recA 1646694..1647848 (-) 1155 WP_078228268.1 recombinase RecA Machinery gene
  SM12261_RS08320 (SM12261_1605) cinA 1647903..1649159 (-) 1257 WP_000642690.1 competence/damage-inducible protein A Machinery gene
  SM12261_RS08325 (SM12261_1606) brpA 1649237..1650292 (-) 1056 WP_000239266.1 biofilm formation/cell division transcriptional regulator BrpA -
  SM12261_RS08330 (SM12261_1607) - 1650300..1650818 (-) 519 WP_000455549.1 GNAT family N-acetyltransferase -
  SM12261_RS08335 (SM12261_1608) tsaE 1650808..1651251 (-) 444 WP_000288249.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
  SM12261_RS08340 (SM12261_1609) comM 1651347..1651979 (-) 633 WP_000970273.1 hypothetical protein Regulator
  SM12261_RS08345 (SM12261_1610) ndk 1652109..1652531 (-) 423 WP_004239114.1 nucleoside-diphosphate kinase -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45122.44 Da        Isoelectric Point: 4.5781

>NTDB_id=510 SM12261_RS08320 WP_000642690.1 1647903..1649159(-) (cinA) [Streptococcus mitis NCTC 12261]
MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGDNEARLLSLLEIASQRSSLVILTGGLGPTEDDLTKQT
LAKFLGKELVFDPQAQEKLDVFFAQRPDYARTPNNERQAQLVEGAIPLPNETGLAVGGILEVDGVTYVVLPGPPSELKPM
VLNQLLPKLMTGSKLHSRVLRFFGIGESQLVTILSDLIDNQTDPTLAPYAKTGEVTLRLSTKASSQEEANQVLDILENQI
LGRQTFEGLSLRDLCYGYGEETSLASIVVEELKRQGKTITSAESLTAGLFQARVADFSGASSIFKGGFVTYSLEEKSKML
DIPVKDLEEQGVVSEFTAQKMAEQARIKTQSDFGLSLTGVAGPDSLEGHPAGTVFIGLAQEQGTEVIKVNIGGRSRADVR
HIAVMHAFNLVRKALLSD

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=510 SM12261_RS08320 WP_000642690.1 1647903..1649159(-) (cinA) [Streptococcus mitis NCTC 12261]
ATGAAAGCAGAAATTATTGCTGTTGGAACAGAGATTTTGACAGGACAGATTGTCAATACCAATGCTCAGTTTTTGTCGGA
AAAACTAGCAGAGATTGGGGTAGATGTATATTTTCAGACGGCTGTAGGAGACAATGAAGCTCGTCTCTTGTCTTTGCTCG
AAATTGCCAGTCAACGTAGTAGTCTAGTGATTTTGACAGGTGGTTTAGGGCCAACTGAGGACGATTTGACCAAACAAACT
CTGGCTAAATTTTTAGGGAAAGAATTAGTCTTTGATCCTCAGGCGCAGGAGAAGTTGGATGTCTTTTTTGCCCAGAGACC
AGACTATGCCCGAACACCGAATAACGAAAGACAAGCTCAACTTGTAGAAGGAGCGATTCCACTGCCAAATGAAACAGGAC
TGGCTGTGGGAGGAATATTGGAAGTCGATGGAGTGACCTACGTTGTCCTTCCAGGTCCACCAAGTGAATTAAAACCTATG
GTTTTAAACCAACTTCTACCCAAGTTGATGACAGGGAGCAAGCTGCATTCCCGAGTTCTTCGTTTCTTTGGGATTGGCGA
GAGCCAGTTGGTTACGATTTTGTCTGATTTGATTGATAATCAAACCGATCCGACCTTGGCCCCTTATGCTAAGACAGGAG
AAGTCACTCTGCGTCTGTCAACAAAAGCTAGCAGCCAAGAAGAGGCGAATCAAGTGCTGGATATCTTGGAAAATCAAATC
TTAGGTCGCCAGACCTTTGAAGGCCTTTCTTTACGAGATCTTTGTTATGGTTATGGGGAAGAAACTAGTTTAGCTAGCAT
TGTGGTAGAAGAACTGAAAAGGCAAGGGAAAACCATCACGTCTGCTGAGAGTTTGACGGCTGGTCTTTTCCAAGCTAGGG
TAGCGGATTTTTCGGGTGCTTCAAGTATATTTAAGGGTGGTTTTGTAACCTATAGCTTGGAGGAAAAATCAAAGATGTTG
GATATTCCTGTCAAGGATTTGGAAGAACAGGGTGTGGTGTCTGAATTTACAGCACAGAAGATGGCTGAGCAGGCACGAAT
CAAGACCCAGTCTGATTTTGGCCTTAGTTTGACTGGTGTGGCAGGGCCAGATAGCCTAGAAGGACATCCAGCTGGGACAG
TCTTCATAGGCTTGGCTCAAGAGCAAGGAACTGAGGTCATCAAGGTCAATATTGGAGGCAGAAGTCGAGCAGATGTACGT
CACATTGCGGTTATGCATGCCTTTAACCTAGTTCGCAAGGCTTTATTAAGTGACTAA

Domains

Predicted by InterproScan.

Probable molybdopterin binding domain (MoCF_biosynth)

(5-169)

Damage-inducible protein CinA KH domain (CinA_KH)

(177-240)

Competence-damaged protein (CinA)

(262-414)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mitis SK321

97.368

100

0.974
  cinA Streptococcus pneumoniae TIGR4

93.301

100

0.933
  cinA Streptococcus pneumoniae Rx1

93.062

100

0.931
  cinA Streptococcus pneumoniae R6

93.062

100

0.931
  cinA Streptococcus pneumoniae D39

92.823

100

0.928
  cinA Streptococcus mutans UA159

71.77

100

0.718
  cinA Streptococcus suis isolate S10

54.567

100

0.578
  cinA Bacillus subtilis subsp. subtilis str. 168

45.694

100

0.459

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]