Detailed information    

experimental Experimentally validated

Overview

Name   cinA   Type   Machinery gene
Locus tag   GPW51_RS00385 Genome accession   NZ_LR738721
Coordinates   66452..67633 (+) Length   393 a.a.
NCBI ID   WP_012774894.1    Uniprot ID   -
Organism   Streptococcus suis isolate S10     
Function   facilitate localization of RecA   
Homologous recombination

Function

The ComX regulon also included genes involved in DNA repair including cinA which we showed to be required for high efficiency transformation.


Genomic Context

Location: 61452..72633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW51_RS00365 mutL 62555..64492 (+) 1938 WP_012774891.1 DNA mismatch repair endonuclease MutL -
  GPW51_RS00370 ruvA 64531..65121 (+) 591 WP_011921679.1 Holliday junction branch migration protein RuvA -
  GPW51_RS00380 - 65846..66415 (+) 570 WP_012774893.1 DNA-3-methyladenine glycosylase I -
  GPW51_RS00385 cinA 66452..67633 (+) 1182 WP_012774894.1 competence/damage-inducible protein A Machinery gene
  GPW51_RS00390 recA 67685..68836 (+) 1152 WP_011922545.1 recombinase RecA Machinery gene
  GPW51_RS00395 spx 69072..69470 (+) 399 WP_002939362.1 transcriptional regulator Spx -
  GPW51_RS00400 - 69570..69836 (+) 267 WP_002939360.1 IreB family regulatory phosphoprotein -
  GPW51_RS00405 ruvX 69836..70255 (+) 420 WP_002939356.1 Holliday junction resolvase RuvX -
  GPW51_RS00410 - 70267..70587 (+) 321 WP_002939343.1 DUF1292 domain-containing protein -
  GPW51_RS00415 - 70795..71373 (+) 579 WP_012774895.1 nucleotidyltransferase family protein -
  GPW51_RS00420 rpsJ 72036..72344 (+) 309 WP_011922546.1 30S ribosomal protein S10 -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 42862.76 Da        Isoelectric Point: 4.3975

>NTDB_id=462 GPW51_RS00385 WP_012774894.1 66452..67633(+) (cinA) [Streptococcus suis isolate S10]
MKAELIAVGTEILTGQIINTNAQFLSEKCAELGIDVYFHTAVGDNEGRLLSTLEVASKRSNMVVLCGGLGPTEDDLTKQT
LATFLGRNLVFDELAMAKLDRFFASRPGRVRTPNNERQAQIVEGSQALQNPAGLAVGGMIEQDGVTYIVLPGPPSELKAM
FSESLLPLLSQSQQQLYSRVLRFFGIGESQLVTVLADVIDKQTDPTLAPYAKVGEVTLRLSTKATSQEEANLRLNQLEED
ILQHDKLADYFYAYGEDNSLVKTVATRLAEKRQTIAIVEQGTGGLLQAELSLALADQPYFSGGKVVGQLGIESGWLSEEA
DCIRQELQADLGLAVSVLIKPESTEDNVLAKVYLTLATPSGISQKEIDLGGYSWQYLRQLACLQAWDFVRNTL

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=462 GPW51_RS00385 WP_012774894.1 66452..67633(+) (cinA) [Streptococcus suis isolate S10]
ATGAAAGCAGAACTAATCGCCGTTGGGACGGAGATTTTGACAGGTCAAATCATTAATACCAATGCTCAGTTCCTTTCGGA
GAAATGTGCAGAGCTGGGAATTGATGTTTATTTTCATACGGCAGTTGGTGACAATGAAGGCCGTTTATTATCAACCCTTG
AAGTAGCCAGCAAGCGGAGTAATATGGTCGTTCTTTGCGGTGGTTTAGGACCAACTGAGGACGACCTGACCAAGCAGACC
TTGGCGACCTTCTTAGGTAGAAATCTTGTGTTTGATGAGTTGGCTATGGCAAAATTAGACCGTTTCTTTGCCAGTCGCCC
AGGTCGTGTCCGTACACCAAATAATGAACGTCAGGCACAGATTGTGGAGGGAAGTCAGGCGCTACAGAATCCAGCTGGTT
TAGCTGTTGGTGGTATGATTGAGCAAGATGGTGTAACCTATATTGTTTTGCCTGGCCCACCAAGTGAGCTCAAGGCCATG
TTTTCTGAGAGTCTCTTACCTTTACTGTCCCAATCTCAGCAGCAACTTTATTCGCGTGTCCTACGCTTTTTTGGAATTGG
CGAAAGCCAGTTGGTGACTGTTTTGGCGGACGTGATTGATAAGCAGACAGACCCAACTCTTGCTCCTTATGCAAAAGTTG
GAGAGGTGACTTTACGCTTGTCCACCAAAGCGACCAGCCAAGAAGAAGCAAATCTACGTTTAAATCAGTTGGAAGAAGAC
ATCTTACAACATGACAAACTGGCAGACTATTTCTATGCCTACGGAGAGGACAATAGTTTGGTCAAAACGGTAGCGACTCG
TTTGGCGGAGAAAAGACAAACCATCGCTATCGTCGAACAGGGGACAGGTGGTCTCTTGCAAGCTGAATTGAGCCTGGCTT
TGGCTGATCAGCCGTATTTTAGCGGAGGAAAAGTCGTCGGTCAGCTAGGGATAGAATCGGGCTGGCTATCAGAGGAAGCT
GACTGCATTCGGCAGGAGCTGCAAGCTGATTTAGGTTTGGCTGTGTCTGTGCTTATCAAACCGGAATCAACAGAGGACAA
CGTACTTGCAAAAGTATACCTCACTTTGGCTACGCCCTCGGGTATTTCCCAAAAAGAGATAGATTTAGGTGGTTATTCGT
GGCAATACCTTCGCCAGCTTGCTTGTCTGCAGGCCTGGGATTTTGTACGAAACACTTTGTGA

Domains

Predicted by InterproScan.

Probable molybdopterin binding domain (MoCF_biosynth)

(5-170)

Competence-damaged protein (CinA)

(258-392)

Damage-inducible protein CinA KH domain (CinA_KH)

(178-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mitis SK321

54.808

100

0.58
  cinA Streptococcus pneumoniae TIGR4

54.808

100

0.58
  cinA Streptococcus pneumoniae Rx1

54.808

100

0.58
  cinA Streptococcus pneumoniae R6

54.808

100

0.58
  cinA Streptococcus mutans UA159

55.474

100

0.58
  cinA Streptococcus pneumoniae D39

54.567

100

0.578
  cinA Streptococcus mitis NCTC 12261

54.567

100

0.578
  cinA Bacillus subtilis subsp. subtilis str. 168

41.379

95.929

0.397

Multiple sequence alignment    



References

[1] Edoardo Zaccaria et al. (2014) Control of competence for DNA transformation in streptococcus suis by genetically transferable pherotypes. PloS One 9(6):e99394. [PMID: 24968201]