Detailed information    

experimental Experimentally validated

Overview

Name   cinA   Type   Machinery gene
Locus tag   SMU_RS09535 Genome accession   NC_004350
Coordinates   1957650..1958906 (-) Length   418 a.a.
NCBI ID   WP_002262393.1    Uniprot ID   Q8DRX2
Organism   Streptococcus mutans UA159     
Function   facilitate localization of RecA   
Homologous recombination

Function

In CSP-supplemented cultures, a two-fold increase in cell viability was noted for SmuCinA relative to UA159 (P < 0.002), suggesting CinA's involvement in the CSP-modulated cell killing response. Relative to UA159, loss of CinA also rendered the mutant hypersensitive to killing by methyl methanesulfonate (MMS), which impairs homologous recombination. Despite our use of a non-polar mutagenesis strategy to knockout cinA, which is the first gene of the multicistronic operon harboring cinA, we noted a drastic reduction in recA expression.


Genomic Context

Location: 1952650..1963906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS09490 (SMU.2077c) - 1952844..1953143 (-) 300 WP_002262384.1 DUF1292 domain-containing protein -
  SMU_RS09495 (SMU.2078c) ruvX 1953224..1953643 (-) 420 WP_002262385.1 Holliday junction resolvase RuvX -
  SMU_RS09500 (SMU.2079c) - 1953640..1953909 (-) 270 WP_002262386.1 IreB family regulatory phosphoprotein -
  SMU_RS09505 (SMU.2080) brsR 1954077..1954511 (+) 435 WP_002262387.1 bacteriocin genes transcriptional regulator BrsR Regulator
  SMU_RS09510 (SMU.2081) brsM 1954524..1954970 (+) 447 WP_002262388.1 bacteriocin genes regulator BrsM Regulator
  SMU_RS09515 - 1954961..1955173 (-) 213 WP_002262389.1 hypothetical protein -
  SMU_RS09520 (SMU.2083c) - 1955300..1955725 (-) 426 WP_002262390.1 hypothetical protein -
  SMU_RS09525 (SMU.2084c) spx 1955976..1956374 (-) 399 WP_002262391.1 transcriptional regulator Spx -
  SMU_RS09530 (SMU.2085) recA 1956459..1957610 (-) 1152 WP_002262392.1 recombinase RecA Machinery gene
  SMU_RS09535 (SMU.2086) cinA 1957650..1958906 (-) 1257 WP_002262393.1 competence/damage-inducible protein A Machinery gene
  SMU_RS09540 (SMU.2087) - 1959055..1959615 (-) 561 WP_002262394.1 DNA-3-methyladenine glycosylase I -
  SMU_RS09545 (SMU.2088) ruvA 1959697..1960290 (-) 594 WP_002262395.1 Holliday junction branch migration protein RuvA -
  SMU_RS09550 (SMU.2089) mutL 1960473..1962428 (-) 1956 WP_002262396.1 DNA mismatch repair endonuclease MutL -
  SMU_RS09555 - 1962653..1962931 (+) 279 Protein_1857 transposase -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45732.54 Da        Isoelectric Point: 6.2714

>NTDB_id=412 SMU_RS09535 WP_002262393.1 1957650..1958906(-) (cinA) [Streptococcus mutans UA159]
MKSEIIAVGTEILTGQIVNTNSQFLSEKFAELGIDVYFQTAVGDNEERLLSVLKIAKERSDLIVLCGGLGPTEDDLTKQT
LAKFLKRELVFDKTAQERLDEFFASRPTSMRTPNNECQAQIIAGSQPLSNKTGLAVGGLLEADGVTYVVLPGPPSELKPM
VNKELLPYLSKTSEKLYSRVLRFFGIGESHLVTLLHDLIAEQTDPTIAPYAKTGEVTIRLSTKAHRQKEADSKLDKLEKK
IITIDNLADYFYGYGEENSLPQVVFDLLKEKGKTITAAESLTAGLFQARLADFAGASDIFKGGFITYSIEEKARMLGIPF
EDLQLHGVVSAFTAEKMAERSRQLTQADLAISLTGVAGPDSLEGQPAGTVFIGLSSSKRTMAIKVLIGGRSRSDVRYIAV
LHAFNLVRQTLLSHKNLV

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=412 SMU_RS09535 WP_002262393.1 1957650..1958906(-) (cinA) [Streptococcus mutans UA159]
ATGAAATCTGAGATCATTGCTGTTGGAACAGAAATTTTAACAGGACAAATTGTTAATACTAATAGTCAATTTTTATCAGA
AAAATTCGCAGAATTAGGAATTGATGTTTATTTTCAAACAGCAGTTGGTGATAATGAAGAACGCCTTTTATCTGTTTTGA
AAATTGCAAAGGAACGTAGTGATTTAATCGTACTTTGTGGTGGTTTGGGACCAACAGAAGATGATTTAACAAAACAAACC
CTAGCAAAATTTTTGAAAAGAGAATTGGTATTTGATAAAACAGCTCAAGAACGTCTAGATGAATTTTTTGCATCACGTCC
AACATCTATGCGCACACCAAATAATGAATGTCAGGCGCAGATTATAGCAGGAAGTCAGCCTTTATCTAATAAAACAGGTT
TAGCAGTTGGGGGGCTTTTGGAGGCAGATGGAGTGACTTATGTTGTATTACCTGGTCCGCCTAGCGAATTGAAGCCTATG
GTTAACAAAGAGTTACTCCCTTATTTATCAAAAACTTCTGAGAAGCTTTATTCTCGTGTGTTACGCTTTTTTGGTATTGG
TGAGAGTCATCTAGTGACCTTGTTGCATGACTTAATAGCGGAGCAAACAGATCCCACAATTGCTCCTTATGCTAAGACAG
GCGAGGTAACAATACGTTTATCAACAAAAGCCCATAGGCAGAAAGAAGCAGATAGTAAGTTAGATAAGTTGGAAAAGAAA
ATTATAACTATTGATAACTTGGCTGATTATTTTTATGGTTATGGTGAAGAAAATTCATTACCACAGGTTGTATTTGATCT
GCTTAAGGAAAAGGGTAAAACGATTACAGCAGCTGAAAGTTTAACAGCAGGCCTTTTTCAGGCAAGATTAGCAGATTTTG
CAGGAGCATCTGATATCTTTAAGGGAGGTTTTATAACTTACAGTATAGAAGAGAAGGCTAGAATGCTAGGTATTCCCTTT
GAAGATCTCCAACTTCATGGGGTTGTTAGTGCTTTTACTGCTGAAAAGATGGCAGAGCGCTCCCGCCAGCTGACTCAAGC
AGATTTGGCTATTTCTTTAACAGGAGTTGCAGGTCCGGATAGCTTAGAAGGTCAGCCGGCAGGGACAGTTTTTATCGGTT
TGTCAAGTTCCAAAAGAACAATGGCAATCAAAGTGTTAATTGGCGGACGCAGTCGGTCTGATGTGCGTTATATCGCTGTT
TTACATGCTTTTAATTTAGTACGTCAAACTTTATTATCTCATAAAAATTTAGTATAA

Domains

Predicted by InterproScan.

Damage-inducible protein CinA KH domain (CinA_KH)

(178-253)

Probable molybdopterin binding domain (MoCF_biosynth)

(5-170)

Competence-damaged protein (CinA)

(259-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 6MR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mitis SK321

72.488

100

0.725
  cinA Streptococcus mitis NCTC 12261

71.77

100

0.718
  cinA Streptococcus pneumoniae TIGR4

70.813

100

0.708
  cinA Streptococcus pneumoniae R6

70.574

100

0.706
  cinA Streptococcus pneumoniae Rx1

70.574

100

0.706
  cinA Streptococcus pneumoniae D39

70.335

100

0.703
  cinA Streptococcus suis isolate S10

55.474

100

0.58
  cinA Bacillus subtilis subsp. subtilis str. 168

46.005

99.279

0.457

Multiple sequence alignment    



References

[1] Richard W Mair et al. (2012) CinA is regulated via ComX to modulate genetic transformation and cell viability in Streptococcus mutans. FEMS Microbiology Letters 331(1):44-52. [PMID: 22428842]