Detailed information NTG0000170

Experimentally validated

Overview

• Name: cinA
• Type: Machinery gene
• Locus tag: SP_RS09755
• Genome accession: NC_003028
• Coordinates: 1844634..1845890 (-)
• Length: 418 a.a.
• NCBI ID: WP_000642718.1
• Uniprot ID: P54184
• Organism: Streptococcus pneumoniae TIGR4
• Function: facilitate localization of RecA Homologous recombination

Function

CinA has been reported to play an important role during competence of Streptococcus pneumoniae. A protein–protein interaction between CinA and RecA was observed in vitro, and the localization of RecA to the membrane of competent S. pneumoniae cells was shown to be dependent on CinA.

Genomic Context

Location: 1839634..1850890

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SP_RS09730	-	1839693..1839899 (-)	207	WP_000366093.1	hypothetical protein	-
SP_RS09735 (SP_1936)	-	1839886..1840047 (-)	162	WP_000289348.1	helix-turn-helix domain-containing protein	-
SP_RS09740 (SP_1937)	lytA	1840405..1841361 (-)	957	WP_000405234.1	N-acetylmuramoyl-L-alanine amidase LytA	Auxiliary factor
SP_RS09745 (SP_1939)	-	1841737..1843107 (-)	1371	WP_001036271.1	MATE family efflux transporter	-
SP_RS09750 (SP_1940)	recA	1843413..1844579 (-)	1167	WP_001085462.1	recombinase RecA	Machinery gene
SP_RS09755 (SP_1941)	cinA	1844634..1845890 (-)	1257	WP_000642718.1	competence/damage-inducible protein A	Machinery gene
SP_RS09760 (SP_1942)	brpA	1845975..1846991 (-)	1017	WP_000239283.1	biofilm formation/cell division transcriptional regulator BrpA	-
SP_RS09765 (SP_1943)	-	1846999..1847517 (-)	519	WP_000455537.1	GNAT family N-acetyltransferase	-
SP_RS09770 (SP_1944)	tsaE	1847507..1847950 (-)	444	WP_000288232.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
SP_RS09775 (SP_1945)	comM	1848036..1848656 (-)	621	WP_000839908.1	hypothetical protein	Regulator
SP_RS09780 (SP_1946)	-	1849039..1849902 (-)	864	WP_001093641.1	helix-turn-helix domain-containing protein	-
SP_RS13695 (SP_1947)	-	1850094..1850264 (+)	171	WP_001227862.1	PhrA family quorum-sensing system peptide	-
SP_RS09790 (SP_1948)	pneA1	1850367..1850591 (+)	225	WP_000181087.1	two-peptide bacteriocin subunit PneA1	-
SP_RS09795 (SP_1949)	pneA2	1850598..1850786 (+)	189	WP_000786759.1	two-peptide bacteriocin subunit PneA2	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 45091.48 Da
• Isoelectric Point: 4.6027

>NTDB_id=170 SP_RS09755 WP_000642718.1 1844634..1845890(-) (cinA) [Streptococcus pneumoniae TIGR4]
MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGDNEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQT
LAKFLGKALVFDPQAQEKLDIFFTLRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLRLSTKASSQEEANQALDILENQI
LDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQGKTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRML
DIPAKNLEEHGVVSEFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIGGRSRADVR
HIAVMHAFNLVRKALLSD

Nucleotide

• Length: 1257 bp

>NTDB_id=170 SP_RS09755 WP_000642718.1 1844634..1845890(-) (cinA) [Streptococcus pneumoniae TIGR4]
ATGAAAGCAGAAATCATTGCTGTTGGAACAGAGATTTTGACAGGGCAGATTGTCAACACCAATGCCCAGTTTTTGTCGGA
AAAACTAGCTGAGATTGGGGTAGATGTATATTTTCAGACGGCTGTAGGAGACAATGAAGTTCGTCTCTTGTCTTTGCTTG
AGATTGCCAGTCAACGTAGCAGTCTGGTGATTTTGACAGGCGGTTTGGGGCCAACTGAGGACGACCTAACCAAACAAACC
CTAGCTAAATTTTTAGGGAAAGCATTAGTCTTTGATCCTCAGGCTCAGGAGAAGTTGGATATCTTTTTTACCCTGCGACC
AGACTATGCCCGAACACCGAATAACGAAAGACAAGCTCAAATTGTAGAAGGAGCGATTCCACTGCCAAACGAAACAGGAC
TGGCTGTTGGAGGAAAATTAGAAGTAGACGGAGTGACCTATGTCGTCCTTCCAGGTCCGCCAAGTGAATTGAAACCCATG
GTCTTAAACCAACTTCTACCCAAGTTGATGACAGGGAGCAAGCTGTATTCCCGAGTTCTTCGTTTCTTTGGGATTGGCGA
GAGCCAGTTGGTTACGATTTTGGCTGATTTAATTGATAATCAGATCGATCCTACCTTGGCCCCTTATGCCAAGACAGGAG
AAGTCACTCTACGTCTGTCAACAAAGGCTAGCAGTCAAGAAGAGGCGAATCAAGCGCTGGATATCTTGGAAAATCAAATC
TTGGACTGCCAGACTTTCGAAGGAATTTCTTTACGAGACTTTTGCTATGGTTATGGGGAAGAAACTAGTTTAGCCAGCAT
TGTGGTAGAAGAACTGAAAAGGCAAGGGAAAACCATCGCGGCTGCAGAGAGTTTGACGGCAGGTCTTTTCCAAGCTACCG
TGGCGAATTTTTCTGGAGTTTCAAGTATATTTAAGGGTGGTTTTGTGACCTATAGCTTGGAGGAAAAATCAAGGATGTTG
GATATTCCTGCCAAGAATTTGGAAGAACATGGTGTGGTGTCTGAATTTACAGCTCAGAAGATGGCTGAGCAGGCACGAAG
CAAGACCCAGTCTGATTTTGGAATTAGTTTGACTGGAGTGGCAGGACCAGATAGCCTAGAAGGACACCCAGTTGGGACAG
TCTTCATAGGCTTGGCGCAAGATCAAGGAACTGAGGTTATCAAGGTTAATATTGGAGGCAGAAGCCGAGCAGATGTACGT
CACATTGCGGTTATGCATGCCTTTAACCTAGTTCGCAAGGCTTTATTAAGTGACTAA

Domains

Predicted by InterProScan.

• Competence-damaged protein (CinA) (262-414)

• Damage-inducible protein CinA KH domain (CinA_KH) (177-240)

• Probable molybdopterin binding domain (MoCF_biosynth) (5-169)

3D structure

• AlphaFold DB: P54184

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
cinA	Streptococcus pneumoniae R6	99.282	100	0.993
cinA	Streptococcus pneumoniae Rx1	99.282	100	0.993
cinA	Streptococcus pneumoniae R36A	99.282	100	0.993
cinA	Streptococcus pneumoniae D39	99.043	100	0.99
cinA	Streptococcus mitis SK321	93.541	100	0.935
cinA	Streptococcus mitis NCTC 12261	93.301	100	0.933
cinA	Streptococcus mutans UA159	70.813	100	0.708
cinA	Streptococcus suis isolate S10	54.808	100	0.58
cinA	Bacillus subtilis subsp. subtilis str. 168	45.455	100	0.457

References

• [1]: Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
• [2]: Ping Luo et al. (2003) Transient association of an alternative sigma factor, ComX, with RNA polymerase during the period of competence for genetic transformation in Streptococcus pneumoniae. Journal of Bacteriology 185(1):349-58. [PMID: 12486073]
• [3]: M S Lee et al. (1999) Identification of a new regulator in Streptococcus pneumoniae linking quorum sensing to competence for genetic transformation. Journal of Bacteriology 181(16):5004-16. [PMID: 10438773]
• [4]: I Mortier-Barrière et al. (1998) Competence-specific induction of recA is required for full recombination proficiency during transformation in Streptococcus pneumoniae. Molecular Microbiology 27(1):159-70. [PMID: 9466264]
• [5]: H R Masure et al. (1998) Membrane targeting of RecA during genetic transformation. Molecular Microbiology 27(4):845-52. [PMID: 9515709]