Detailed information    

experimental Experimentally validated

Overview

Name   cinA   Type   Machinery gene
Locus tag   SP_RS09755 Genome accession   NC_003028
Coordinates   1844634..1845890 (-) Length   418 a.a.
NCBI ID   WP_000642718.1    Uniprot ID   P54184
Organism   Streptococcus pneumoniae TIGR4     
Function   facilitate localization of RecA   
Homologous recombination

Function

CinA has been reported to play an important role during competence of Streptococcus pneumoniae. A protein–protein interaction between CinA and RecA was observed in vitro, and the localization of RecA to the membrane of competent S. pneumoniae cells was shown to be dependent on CinA.


Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1837532..1859723 1844634..1845890 within 0


Gene organization within MGE regions


Location: 1837532..1859723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS09715 (SP_1933) - 1837532..1838326 (-) 795 WP_001101210.1 hypothetical protein -
  SP_RS09720 (SP_1934) - 1838734..1839159 (-) 426 WP_000204063.1 hypothetical protein -
  SP_RS09725 (SP_1935) - 1839288..1839647 (-) 360 WP_000749819.1 ImmA/IrrE family metallo-endopeptidase -
  SP_RS09730 - 1839693..1839899 (-) 207 WP_000366093.1 hypothetical protein -
  SP_RS09735 (SP_1936) - 1839886..1840047 (-) 162 WP_000289348.1 helix-turn-helix domain-containing protein -
  SP_RS09740 (SP_1937) lytA 1840405..1841361 (-) 957 WP_000405234.1 N-acetylmuramoyl-L-alanine amidase LytA Auxiliary factor
  SP_RS09745 (SP_1939) - 1841737..1843107 (-) 1371 WP_001036271.1 MATE family efflux transporter -
  SP_RS09750 (SP_1940) recA 1843413..1844579 (-) 1167 WP_001085462.1 recombinase RecA Machinery gene
  SP_RS09755 (SP_1941) cinA 1844634..1845890 (-) 1257 WP_000642718.1 competence/damage-inducible protein A Machinery gene
  SP_RS09760 (SP_1942) brpA 1845975..1846991 (-) 1017 WP_000239283.1 biofilm formation/cell division transcriptional regulator BrpA -
  SP_RS09765 (SP_1943) - 1846999..1847517 (-) 519 WP_000455537.1 GNAT family N-acetyltransferase -
  SP_RS09770 (SP_1944) tsaE 1847507..1847950 (-) 444 WP_000288232.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
  SP_RS09775 (SP_1945) comM 1848036..1848656 (-) 621 WP_000839908.1 hypothetical protein Regulator
  SP_RS09780 (SP_1946) - 1849039..1849902 (-) 864 WP_001093641.1 helix-turn-helix domain-containing protein -
  SP_RS13695 (SP_1947) - 1850094..1850264 (+) 171 WP_001227862.1 PhrA family quorum-sensing system peptide -
  SP_RS09790 (SP_1948) pneA1 1850367..1850591 (+) 225 WP_000181087.1 two-peptide bacteriocin subunit PneA1 -
  SP_RS09795 (SP_1949) pneA2 1850598..1850786 (+) 189 WP_000786759.1 two-peptide bacteriocin subunit PneA2 -
  SP_RS09800 (SP_1950) - 1850902..1853892 (+) 2991 WP_000875573.1 type 2 lanthipeptide synthetase LanM family protein -
  SP_RS09805 (SP_1951) - 1854242..1854946 (+) 705 WP_001033170.1 flavodoxin family protein -
  SP_RS09810 (SP_1952) - 1854927..1855856 (+) 930 WP_000912954.1 M50 family metallopeptidase -
  SP_RS09815 (SP_1953) - 1855853..1857961 (+) 2109 WP_000701298.1 peptidase domain-containing ABC transporter -
  SP_RS09820 (SP_1954) - 1857985..1859397 (+) 1413 WP_000699968.1 S8 family peptidase -
  SP_RS13515 (SP_1955) - 1859451..1859723 (+) 273 WP_000939671.1 hypothetical protein -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45091.48 Da        Isoelectric Point: 4.6027

>NTDB_id=170 SP_RS09755 WP_000642718.1 1844634..1845890(-) (cinA) [Streptococcus pneumoniae TIGR4]
MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGDNEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQT
LAKFLGKALVFDPQAQEKLDIFFTLRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLRLSTKASSQEEANQALDILENQI
LDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQGKTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRML
DIPAKNLEEHGVVSEFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIGGRSRADVR
HIAVMHAFNLVRKALLSD

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=170 SP_RS09755 WP_000642718.1 1844634..1845890(-) (cinA) [Streptococcus pneumoniae TIGR4]
ATGAAAGCAGAAATCATTGCTGTTGGAACAGAGATTTTGACAGGGCAGATTGTCAACACCAATGCCCAGTTTTTGTCGGA
AAAACTAGCTGAGATTGGGGTAGATGTATATTTTCAGACGGCTGTAGGAGACAATGAAGTTCGTCTCTTGTCTTTGCTTG
AGATTGCCAGTCAACGTAGCAGTCTGGTGATTTTGACAGGCGGTTTGGGGCCAACTGAGGACGACCTAACCAAACAAACC
CTAGCTAAATTTTTAGGGAAAGCATTAGTCTTTGATCCTCAGGCTCAGGAGAAGTTGGATATCTTTTTTACCCTGCGACC
AGACTATGCCCGAACACCGAATAACGAAAGACAAGCTCAAATTGTAGAAGGAGCGATTCCACTGCCAAACGAAACAGGAC
TGGCTGTTGGAGGAAAATTAGAAGTAGACGGAGTGACCTATGTCGTCCTTCCAGGTCCGCCAAGTGAATTGAAACCCATG
GTCTTAAACCAACTTCTACCCAAGTTGATGACAGGGAGCAAGCTGTATTCCCGAGTTCTTCGTTTCTTTGGGATTGGCGA
GAGCCAGTTGGTTACGATTTTGGCTGATTTAATTGATAATCAGATCGATCCTACCTTGGCCCCTTATGCCAAGACAGGAG
AAGTCACTCTACGTCTGTCAACAAAGGCTAGCAGTCAAGAAGAGGCGAATCAAGCGCTGGATATCTTGGAAAATCAAATC
TTGGACTGCCAGACTTTCGAAGGAATTTCTTTACGAGACTTTTGCTATGGTTATGGGGAAGAAACTAGTTTAGCCAGCAT
TGTGGTAGAAGAACTGAAAAGGCAAGGGAAAACCATCGCGGCTGCAGAGAGTTTGACGGCAGGTCTTTTCCAAGCTACCG
TGGCGAATTTTTCTGGAGTTTCAAGTATATTTAAGGGTGGTTTTGTGACCTATAGCTTGGAGGAAAAATCAAGGATGTTG
GATATTCCTGCCAAGAATTTGGAAGAACATGGTGTGGTGTCTGAATTTACAGCTCAGAAGATGGCTGAGCAGGCACGAAG
CAAGACCCAGTCTGATTTTGGAATTAGTTTGACTGGAGTGGCAGGACCAGATAGCCTAGAAGGACACCCAGTTGGGACAG
TCTTCATAGGCTTGGCGCAAGATCAAGGAACTGAGGTTATCAAGGTTAATATTGGAGGCAGAAGCCGAGCAGATGTACGT
CACATTGCGGTTATGCATGCCTTTAACCTAGTTCGCAAGGCTTTATTAAGTGACTAA

Domains

Predicted by InterproScan.

Competence-damaged protein (CinA)

(262-414)

Damage-inducible protein CinA KH domain (CinA_KH)

(177-240)

Probable molybdopterin binding domain (MoCF_biosynth)

(5-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P54184

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus pneumoniae R6

99.282

100

0.993
  cinA Streptococcus pneumoniae Rx1

99.282

100

0.993
  cinA Streptococcus pneumoniae D39

99.043

100

0.99
  cinA Streptococcus mitis SK321

93.541

100

0.935
  cinA Streptococcus mitis NCTC 12261

93.301

100

0.933
  cinA Streptococcus mutans UA159

70.813

100

0.708
  cinA Streptococcus suis isolate S10

54.808

100

0.58
  cinA Bacillus subtilis subsp. subtilis str. 168

45.455

100

0.457

Multiple sequence alignment    



References

[1] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
[2] Ping Luo et al. (2003) Transient association of an alternative sigma factor, ComX, with RNA polymerase during the period of competence for genetic transformation in Streptococcus pneumoniae. Journal of Bacteriology 185(1):349-58. [PMID: 12486073]
[3] M S Lee et al. (1999) Identification of a new regulator in Streptococcus pneumoniae linking quorum sensing to competence for genetic transformation. Journal of Bacteriology 181(16):5004-16. [PMID: 10438773]
[4] I Mortier-Barrière et al. (1998) Competence-specific induction of recA is required for full recombination proficiency during transformation in Streptococcus pneumoniae. Molecular Microbiology 27(1):159-70. [PMID: 9466264]
[5] H R Masure et al. (1998) Membrane targeting of RecA during genetic transformation. Molecular Microbiology 27(4):845-52. [PMID: 9515709]