Detailed information
Experimentally validated
Overview
| Name | recA | Type | Machinery gene |
| Locus tag | SPD_RS09265 | Genome accession | NC_008533 |
| Coordinates | 1732611..1733777 (-) | Length | 388 a.a. |
| NCBI ID | WP_001085462.1 | Uniprot ID | P0A451 |
| Organism | Streptococcus pneumoniae D39 | ||
| Function | homologous recombination Homologous recombination |
||
Function
RecA is the central recombinase enzyme essential for homologous recombination during natural transformation. It binds single-stranded DNA (ssDNA) internalized by the competence machinery, forms nucleoprotein filaments, and catalyzes strand pairing and exchange between the exogenous DNA and the host chromosome, enabling genomic integration of transformed DNA.
Genomic Context
Location: 1727611..1738777
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SPD_RS09230 (SPD_1735) | - | 1727918..1728343 (-) | 426 | WP_000204065.1 | hypothetical protein | - |
| SPD_RS09235 (SPD_1736) | - | 1728472..1728831 (-) | 360 | WP_000749822.1 | ImmA/IrrE family metallo-endopeptidase | - |
| SPD_RS09240 | - | 1728877..1729083 (-) | 207 | WP_000366091.1 | hypothetical protein | - |
| SPD_RS09245 | - | 1729070..1729243 (-) | 174 | WP_000289349.1 | helix-turn-helix domain-containing protein | - |
| SPD_RS09250 (SPD_1737) | lytA | 1729602..1730558 (-) | 957 | WP_000405235.1 | N-acetylmuramoyl-L-alanine amidase LytA | Auxiliary factor |
| SPD_RS09255 (SPD_1738) | - | 1730934..1732304 (-) | 1371 | WP_001036271.1 | MATE family efflux transporter | - |
| SPD_RS09265 (SPD_1739) | recA | 1732611..1733777 (-) | 1167 | WP_001085462.1 | recombinase RecA | Machinery gene |
| SPD_RS09270 (SPD_1740) | cinA | 1733832..1735088 (-) | 1257 | WP_000642701.1 | competence/damage-inducible protein A | Machinery gene |
| SPD_RS09275 (SPD_1741) | brpA | 1735173..1736189 (-) | 1017 | WP_000239282.1 | biofilm formation/cell division transcriptional regulator BrpA | - |
| SPD_RS09280 (SPD_1742) | - | 1736197..1736715 (-) | 519 | WP_000455537.1 | GNAT family N-acetyltransferase | - |
| SPD_RS09285 (SPD_1743) | tsaE | 1736705..1737148 (-) | 444 | WP_000288232.1 | tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE | - |
| SPD_RS09290 (SPD_1744) | comM | 1737234..1737854 (-) | 621 | WP_000839908.1 | hypothetical protein | Regulator |
Sequence
Protein
Download Length: 388 a.a. Molecular weight: 41949.78 Da Isoelectric Point: 4.8403
>NTDB_id=228 SPD_RS09265 WP_001085462.1 1732611..1733777(-) (recA) [Streptococcus pneumoniae D39]
MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGERAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGP
ESSGKTTVALHAVAQAQKEGGIAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGNPETTPGGRALKFYASVRLDVRG
NTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAVVEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSE
NAKKYLAEHPEIFDEIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE
MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGERAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGP
ESSGKTTVALHAVAQAQKEGGIAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGNPETTPGGRALKFYASVRLDVRG
NTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAVVEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSE
NAKKYLAEHPEIFDEIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE
Nucleotide
Download Length: 1167 bp
>NTDB_id=228 SPD_RS09265 WP_001085462.1 1732611..1733777(-) (recA) [Streptococcus pneumoniae D39]
ATGGCGAAAAAACCAAAAAAATTAGAAGAAATTTCAAAAAAATTTGGGGCAGAACGTGAAAAGGCCTTGAATGACGCTCT
TAAATTGATTGAGAAAGACTTTGGTAAAGGATCAATCATGCGTTTGGGTGAACGTGCGGAGCAAAAGGTGCAAGTGATGA
GCTCAGGTTCTTTAGCTCTTGACATTGCCCTTGGCTCAGGTGGTTATCCTAAGGGACGTATCATCGAAATCTATGGCCCA
GAGTCATCTGGTAAGACAACGGTTGCCCTTCATGCAGTTGCACAAGCGCAAAAAGAAGGTGGGATTGCTGCCTTTATCGA
TGCGGAACATGCCCTTGATCCAGCTTATGCTGCGGCCCTTGGTGTCAATATTGACGAATTGCTCTTGTCTCAACCAGACT
CAGGAGAGCAAGGTCTTGAGATTGCGGGAAAATTGATTGACTCAGGTGCAGTTGATCTTGTCGTAGTCGACTCAGTTGCT
GCCCTTGTTCCTCGTGCGGAAATTGATGGAGATATCGGAGATAGCCATGTTGGTTTGCAGGCTCGTATGATGAGCCAGGC
CATGCGTAAACTTGGCGCCTCTATCAATAAAACCAAAACAATTGCCATTTTTATCAACCAATTGCGTGAAAAAGTTGGAG
TGATGTTTGGAAATCCAGAAACAACACCGGGCGGACGTGCTTTGAAATTCTATGCTTCAGTCCGCTTGGATGTTCGTGGT
AATACACAAATTAAGGGAACTGGTGATCAAAAAGAAACCAATGTCGGTAAAGAAACTAAGATTAAGGTTGTAAAAAATAA
GGTAGCTCCACCGTTTAAGGAAGCCGTAGTTGAAATTATGTACGGAGAAGGAATTTCTAAGACTGGTGAGCTTTTGAAGA
TTGCAAGCGATTTGGATATTATCAAAAAAGCAGGGGCTTGGTATTCTTACAAAGATGAAAAAATTGGGCAAGGTTCTGAG
AATGCTAAGAAATACTTGGCAGAGCACCCAGAAATCTTTGATGAAATTGATAAGCAAGTCCGTTCTAAATTTGGCTTGAT
TGATGGAGAAGAAGTTTCAGAACAAGATACTGAAAACAAAAAAGATGAGCCAAAGAAAGAAGAAGCAGTGAATGAAGAAG
TTCCGCTTGACTTAGGCGATGAACTTGAAATCGAAATTGAAGAATAA
ATGGCGAAAAAACCAAAAAAATTAGAAGAAATTTCAAAAAAATTTGGGGCAGAACGTGAAAAGGCCTTGAATGACGCTCT
TAAATTGATTGAGAAAGACTTTGGTAAAGGATCAATCATGCGTTTGGGTGAACGTGCGGAGCAAAAGGTGCAAGTGATGA
GCTCAGGTTCTTTAGCTCTTGACATTGCCCTTGGCTCAGGTGGTTATCCTAAGGGACGTATCATCGAAATCTATGGCCCA
GAGTCATCTGGTAAGACAACGGTTGCCCTTCATGCAGTTGCACAAGCGCAAAAAGAAGGTGGGATTGCTGCCTTTATCGA
TGCGGAACATGCCCTTGATCCAGCTTATGCTGCGGCCCTTGGTGTCAATATTGACGAATTGCTCTTGTCTCAACCAGACT
CAGGAGAGCAAGGTCTTGAGATTGCGGGAAAATTGATTGACTCAGGTGCAGTTGATCTTGTCGTAGTCGACTCAGTTGCT
GCCCTTGTTCCTCGTGCGGAAATTGATGGAGATATCGGAGATAGCCATGTTGGTTTGCAGGCTCGTATGATGAGCCAGGC
CATGCGTAAACTTGGCGCCTCTATCAATAAAACCAAAACAATTGCCATTTTTATCAACCAATTGCGTGAAAAAGTTGGAG
TGATGTTTGGAAATCCAGAAACAACACCGGGCGGACGTGCTTTGAAATTCTATGCTTCAGTCCGCTTGGATGTTCGTGGT
AATACACAAATTAAGGGAACTGGTGATCAAAAAGAAACCAATGTCGGTAAAGAAACTAAGATTAAGGTTGTAAAAAATAA
GGTAGCTCCACCGTTTAAGGAAGCCGTAGTTGAAATTATGTACGGAGAAGGAATTTCTAAGACTGGTGAGCTTTTGAAGA
TTGCAAGCGATTTGGATATTATCAAAAAAGCAGGGGCTTGGTATTCTTACAAAGATGAAAAAATTGGGCAAGGTTCTGAG
AATGCTAAGAAATACTTGGCAGAGCACCCAGAAATCTTTGATGAAATTGATAAGCAAGTCCGTTCTAAATTTGGCTTGAT
TGATGGAGAAGAAGTTTCAGAACAAGATACTGAAAACAAAAAAGATGAGCCAAAGAAAGAAGAAGCAGTGAATGAAGAAG
TTCCGCTTGACTTAGGCGATGAACTTGAAATCGAAATTGAAGAATAA
Similar proteins
Only experimentally validated proteins are listed.
Multiple sequence alignment
References
| [1] | Calum H G Johnston et al. (2023) The RecA-directed recombination pathway of natural transformation initiates at chromosomal replication forks in the pneumococcus. Proceedings of The National Academy of Sciences of The United States of America 120(8):e2213867120. [PMID: 36795748] |
| [2] | Maud Hertzog et al. (2023) Assembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae. Nucleic Acids Research 51(6):2800-2817. [PMID: 36806960] |
| [3] | Johnny Lisboa et al. (2014) Molecular determinants of the DprA-RecA interaction for nucleation on ssDNA. Nucleic Acids Research 42(11):7395-408. [PMID: 24782530] |
| [4] | Nicolas Mirouze et al. (2013) Direct involvement of DprA, the transformation-dedicated RecA loader, in the shut-off of pneumococcal competence. Proceedings of The National Academy of Sciences of The United States of America 110(11):E1035-44. [PMID: 23440217] |
| [5] | V Sistek et al. (2012) Development of a real-time PCR assay for the specific detection and identification of Streptococcus pseudopneumoniae using the recA gene. Clinical Microbiology And Infection : The Official Publication of The European Society of Clinical Microbiology And Infectious Diseases 18(11):1089-96. [PMID: 22022828] |
| [6] | Diane E Grove et al. (2012) Stimulation of the Streptococcus pneumoniae RecA protein-promoted three-strand exchange reaction by the competence-specific SsbB protein. Biochemical And Biophysical Research Communications 424(1):40-4. [PMID: 22713474] |
| [7] | Sophie Quevillon-Cheruel et al. (2012) Structure-function analysis of pneumococcal DprA protein reveals that dimerization is crucial for loading RecA recombinase onto DNA during transformation. Proceedings of The National Academy of Sciences of The United States of America 109(37):E2466-75. [PMID: 22904190] |
| [8] | Isabelle Mortier-Barrière et al. (2007) A key presynaptic role in transformation for a widespread bacterial protein: DprA conveys incoming ssDNA to RecA. Cell 130(5):824-36. [PMID: 17803906] |
| [9] | Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980] |
| [10] | Francine S Katz et al. (2003) Three-strand exchange by the Escherichia coli RecA protein using ITP as a nucleotide cofactor: mechanistic parallels with the ATP-dependent reaction of the RecA protein from Streptococcus pneumoniae. The Journal of Biological Chemistry 278(38):35889-96. [PMID: 12842880] |
| [11] | Mathieu Bergé et al. (2003) Transformation of Streptococcus pneumoniae relies on DprA- and RecA-dependent protection of incoming DNA single strands. Molecular Microbiology 50(2):527-36. [PMID: 14617176] |
| [12] | Scott E Steffen et al. (2002) Complete inhibition of Streptococcus pneumoniae RecA protein-catalyzed ATP hydrolysis by single-stranded DNA-binding protein (SSB protein): implications for the mechanism of SSB protein-stimulated DNA strand exchange. The Journal of Biological Chemistry 277(17):14493-500. [PMID: 11854290] |
| [13] | Mohammad A Hedayati et al. (2002) Effect of the Streptococcus pneumoniae MmsA protein on the RecA protein-promoted three-strand exchange reaction. Implications for the mechanism of transformational recombination. The Journal of Biological Chemistry 277(28):24863-9. [PMID: 11960988] |
| [14] | S E Steffen et al. (2000) Purification and characterization of the RecA protein from Streptococcus pneumoniae. Archives of Biochemistry And Biophysics 382(2):303-9. [PMID: 11068882] |
| [15] | I Mortier-Barrière et al. (1998) Competence-specific induction of recA is required for full recombination proficiency during transformation in Streptococcus pneumoniae. Molecular Microbiology 27(1):159-70. [PMID: 9466264] |
| [16] | I Mortier-Barriere et al. (1997) Control of recombination rate during transformation of Streptococcus pneumoniae: an overview. Microbial Drug Resistance (Larchmont, N.Y.) 3(3):233-42. [PMID: 9270992] |
| [17] | B Martin et al. (1995) The recA gene of Streptococcus pneumoniae is part of a competence-induced operon and controls lysogenic induction. Molecular Microbiology 15(2):367-79. [PMID: 7538190] |
| [18] | B Martin et al. (1995) The recA gene of Streptococcus pneumoniae is part of a competence-induced operon and controls an SOS regulon. Developments in Biological Standardization 85:293-300. [PMID: 8586192] |
| [19] | B Martin et al. (1992) Identification of the recA gene of Streptococcus pneumoniae. Nucleic Acids Research 20(23):6412. [PMID: 1475203] |