Detailed information    

experimental Experimentally validated

Overview

Name   recA   Type   Machinery gene
Locus tag   GTF74_RS11550 Genome accession   NZ_CP047303
Coordinates   2552452..2553516 (-) Length   354 a.a.
NCBI ID   WP_000344154.1    Uniprot ID   A0A0K9UMZ1
Organism   Vibrio cholerae O1 biovar El Tor strain E7946     
Function   homologous recombination   
Homologous recombination

Function

SbcB directly interacts with the primary bacterial recombinase, RecA, to promote homologous recombination during NT in a manner that does not rely on its canonical exonuclease activity.


Genomic Context

Location: 2547452..2558516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTF74_RS11535 (GTF74_11540) - 2547715..2548902 (-) 1188 WP_001883372.1 aspartate kinase -
  GTF74_RS11540 (GTF74_11545) alaS 2549106..2551688 (-) 2583 WP_000481017.1 alanine--tRNA ligase -
  GTF74_RS11545 (GTF74_11550) recX 2551861..2552319 (-) 459 WP_000006855.1 recombination regulator RecX -
  GTF74_RS11550 (GTF74_11555) recA 2552452..2553516 (-) 1065 WP_000344154.1 recombinase RecA Machinery gene
  GTF74_RS11555 (GTF74_11560) pncC 2553650..2554156 (-) 507 WP_000853811.1 nicotinamide-nucleotide amidase -
  GTF74_RS11560 (GTF74_11565) - 2554323..2555453 (-) 1131 WP_000027776.1 sulfate/molybdate ABC transporter ATP-binding protein -
  GTF74_RS11565 (GTF74_11570) cysW 2555450..2556313 (-) 864 WP_000951362.1 sulfate ABC transporter permease subunit CysW -
  GTF74_RS11570 (GTF74_11575) cysT 2556324..2557175 (-) 852 WP_000531810.1 sulfate/thiosulfate ABC transporter permease CysT -
  GTF74_RS11575 (GTF74_11580) cysP 2557252..2558253 (-) 1002 WP_001079835.1 thiosulfate ABC transporter substrate-binding protein CysP -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sbcB recA positive effect

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38249.92 Da        Isoelectric Point: 5.0766

>NTDB_id=1142 GTF74_RS11550 WP_000344154.1 2552452..2553516(-) (recA) [Vibrio cholerae O1 biovar El Tor strain E7946]
MDENKQKALAAALGQIEKQFGKGSIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRIVEIFGPESSGKTTLTLELIA
AAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGD
SHMGLQARMLSQAMRKLTGNLKQSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNE
TRIKVVKNKIAAPFKEANTQIMYGQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQGKANACKYLKENPEIAKTLDK
KLREMLLNPENMQLIAETSSAADDVEFGAVPEEF

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1142 GTF74_RS11550 WP_000344154.1 2552452..2553516(-) (recA) [Vibrio cholerae O1 biovar El Tor strain E7946]
ATGGACGAGAATAAACAGAAGGCACTTGCGGCCGCCCTCGGTCAAATTGAAAAGCAATTTGGTAAAGGCTCCATCATGCG
CTTAGGCGACAACCGCGCGATGGATGTAGAAACCATTTCGACCGGTTCTCTGTCTCTGGATATCGCGTTGGGTGCTGGTG
GTCTACCAATGGGACGTATCGTTGAGATCTTCGGCCCTGAATCTTCGGGTAAAACGACACTGACTCTGGAACTGATTGCT
GCTGCACAACGTGAAGGTAAAACCTGTGCGTTTATCGATGCCGAGCACGCACTGGATCCGGTTTATGCCAAAAAATTGGG
CGTGAATATCGATGAGCTACTGGTTTCTCAGCCAGATACCGGCGAGCAAGCACTGGAAATTTGTGATGCACTGGCTCGCT
CTGGTGCTGTGGATGTCATCGTTGTTGACTCAGTAGCGGCTTTGACACCAAAAGCGGAAATTGAAGGCGAAATGGGCGAT
AGCCACATGGGTCTGCAAGCGCGTATGTTGTCGCAAGCAATGCGTAAACTGACGGGTAACCTCAAGCAATCCAACTGTAT
GTGTATCTTCATCAACCAAATTCGTATGAAGATTGGTGTGATGTTTGGTAACCCAGAAACTACCACTGGCGGTAACGCAC
TGAAATTCTACGCTTCTGTTCGTTTGGATATTCGCCGTACTGGCGCAATCAAAGAAGGCGAAGAAGTGGTGGGTAACGAA
ACCCGTATCAAGGTTGTGAAGAACAAGATTGCTGCGCCGTTTAAAGAAGCGAACACTCAGATCATGTATGGCCAAGGTTT
CAATCGTGAAGGTGAATTGATCGATCTTGGTGTGAAACACAAAATGGTTGAGAAGTCAGGCGCTTGGTACAGCTATAACG
GCGATAAAATCGGCCAAGGTAAAGCGAATGCCTGCAAGTATCTGAAAGAAAACCCAGAAATTGCGAAAACTCTAGATAAA
AAACTGCGTGAAATGCTGTTAAACCCAGAAAACATGCAGCTGATCGCTGAAACATCTTCTGCGGCGGATGACGTCGAATT
TGGCGCGGTGCCAGAAGAGTTTTAA

Domains

Predicted by InterproScan.

recA bacterial DNA recombination protein (RecA)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K9UMZ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Vibrio cholerae strain A1552

100

100

1
  recA Pseudomonas stutzeri DSM 10701

75.841

94.236

0.715
  recA Acinetobacter baylyi ADP1

70.69

99.713

0.705
  recA Acinetobacter baumannii D1279779

73.7

93.696

0.691
  recA Glaesserella parasuis strain SC1401

69.516

99.153

0.689
  recA Neisseria gonorrhoeae strain FA1090

66.949

100

0.681
  recA Neisseria gonorrhoeae MS11

66.949

100

0.681
  recA Neisseria gonorrhoeae MS11

66.949

100

0.681
  recA Ralstonia pseudosolanacearum GMI1000

69.822

96.023

0.67
  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

64.506

94.461

0.609
  recA Bacillus subtilis subsp. subtilis str. 168

60.465

98.851

0.598
  recA Helicobacter pylori strain NCTC11637

61.31

96.83

0.594
  recA Helicobacter pylori 26695

61.012

96.83

0.591
  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

61.027

96.501

0.589
  recA Streptococcus pneumoniae Rx1

62.848

91.243

0.573
  recA Streptococcus pneumoniae D39

62.848

91.243

0.573
  recA Streptococcus pneumoniae R6

62.848

91.243

0.573
  recA Streptococcus pneumoniae TIGR4

62.848

91.243

0.573
  recA Streptococcus mitis NCTC 12261

62.229

91.243

0.568
  recA Streptococcus mitis SK321

61.92

91.243

0.565
  recA Streptococcus mutans UA159

61.231

91.808

0.562
  recA Lactococcus lactis subsp. cremoris KW2

60.991

91.243

0.556
  recA Streptococcus pyogenes NZ131

60.615

91.808

0.556
  recA Latilactobacillus sakei subsp. sakei 23K

60.748

90.678

0.551
  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

62.3

88.418

0.551

Multiple sequence alignment    



References

[1] Triana N Dalia et al. (2025) SbcB facilitates natural transformation in Vibrio cholerae in an exonuclease-independent manner. Journal of Bacteriology 207(1):e0041924. [PMID: 39670763]