Detailed information    

experimental Experimentally validated

Overview

Name   sbcB   Type   Machinery gene
Locus tag   GTF74_RS08270 Genome accession   NZ_CP047303
Coordinates   1819603..1821027 (+) Length   474 a.a.
NCBI ID   WP_001183680.1    Uniprot ID   A0A0H3AJR3
Organism   Vibrio cholerae O1 biovar El Tor strain E7946     
Function   promote homologous recombination   
Homologous recombination

Function

SbcB directly interacts with the primary bacterial recombinase, RecA, to promote homologous recombination during NT in a manner that does not rely on its canonical exonuclease activity.


Genomic Context

Location: 1814603..1826027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTF74_RS08250 (GTF74_08255) - 1814871..1815656 (-) 786 WP_000718969.1 adenosylcobinamide-GDP ribazoletransferase -
  GTF74_RS08255 (GTF74_08260) cobT 1815656..1816681 (-) 1026 WP_000887084.1 nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase -
  GTF74_RS08260 (GTF74_08265) msrB 1817292..1817738 (-) 447 WP_000935885.1 peptide-methionine (R)-S-oxide reductase MsrB -
  GTF74_RS08265 (GTF74_08270) - 1818084..1819457 (+) 1374 WP_000117953.1 L-cystine transporter -
  GTF74_RS08270 (GTF74_08275) sbcB 1819603..1821027 (+) 1425 WP_001183680.1 exodeoxyribonuclease I Machinery gene
  GTF74_RS08275 (GTF74_08280) - 1821059..1821433 (+) 375 WP_001881007.1 CidA/LrgA family protein -
  GTF74_RS08280 (GTF74_08285) - 1821424..1822098 (+) 675 WP_000266245.1 LrgB family protein -
  GTF74_RS08285 (GTF74_08290) cdd 1822268..1823155 (+) 888 WP_001245905.1 cytidine deaminase -
  GTF74_RS08290 (GTF74_08295) - 1823314..1824189 (+) 876 WP_000462872.1 AEC family transporter -
  GTF74_RS08295 (GTF74_08300) purT 1824343..1825518 (+) 1176 WP_000468082.1 phosphoribosylglycinamide formyltransferase 2 -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sbcB recA positive effect

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54598.02 Da        Isoelectric Point: 6.0792

>NTDB_id=1141 GTF74_RS08270 WP_001183680.1 1819603..1821027(+) (sbcB) [Vibrio cholerae O1 biovar El Tor strain E7946]
MQQEHQPTFFFFDYETWGVNPAKDRPSQFAGVRTDMDFNIIGEPLVIYCQLPNDYLPAPEAALITGITPQKANSQGLAEP
EFIARIHAELSTPNTINLGYNSIRFDDEVTRYTCYRNFIDPYAWSWQHGNSRWDLLDVLRACHALRPEGIEWPENDDGFT
SFKLEHLSVANGIEHSNAHDAMADVIATIELAKKVKAAQPKLFDYFFTMRHKRKLTELIDIVNQTPLMHVSGMLGRERHY
TSWIVPIAWHPTNQNAVIVVDLGKDPQPLLELDAETLQARLYTRYEDLAPDELPVPVKLVHLNKCPILAPAKTLTAENAQ
KIGIDREQCLAHLNVIRQHPEIREKLVTVFSQEREFNQESDVDSQLYAGFFSPADRAAMEIIRTTAPENLGSLAIRVADK
RIEPLLFRYRARHYPHTLTDTEQRRWAAHCRDYFERNLEGYMLNLENLVHEHQADPKKMAILKSVYHYVEKLAC

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=1141 GTF74_RS08270 WP_001183680.1 1819603..1821027(+) (sbcB) [Vibrio cholerae O1 biovar El Tor strain E7946]
ATGCAGCAAGAACACCAGCCCACATTCTTCTTTTTCGACTACGAGACTTGGGGAGTCAATCCCGCAAAAGATCGTCCTAG
TCAGTTTGCGGGTGTGCGTACCGATATGGACTTCAACATCATCGGCGAGCCTTTGGTAATTTACTGTCAGCTGCCCAACG
ACTATTTGCCCGCCCCTGAGGCCGCATTGATCACAGGGATCACCCCTCAAAAAGCCAATAGCCAAGGTCTCGCGGAGCCC
GAATTTATCGCGCGTATTCATGCTGAGCTCTCCACACCCAATACCATCAACCTAGGTTATAACAGCATACGTTTTGATGA
TGAAGTGACGCGCTACACCTGCTATCGCAACTTTATCGACCCCTATGCTTGGAGCTGGCAACACGGCAATTCCCGTTGGG
ATCTGCTGGATGTGCTTCGCGCGTGTCATGCTTTGCGTCCTGAAGGTATCGAGTGGCCGGAAAATGATGACGGCTTCACC
AGCTTTAAACTGGAACATCTTTCTGTTGCCAACGGCATTGAGCACAGCAACGCGCACGATGCGATGGCCGATGTGATTGC
CACCATTGAACTGGCGAAAAAAGTCAAAGCCGCGCAGCCGAAATTGTTCGACTACTTCTTCACTATGCGCCACAAACGAA
AGCTGACAGAACTGATTGATATTGTGAACCAAACTCCGCTGATGCATGTTTCTGGCATGCTTGGGCGTGAGCGTCACTAC
ACTAGCTGGATTGTGCCTATTGCTTGGCATCCAACCAATCAAAATGCGGTGATTGTGGTGGATTTGGGTAAAGACCCTCA
GCCGCTGCTGGAGCTCGATGCCGAAACGCTGCAAGCGCGTCTCTATACGCGGTATGAAGACCTAGCGCCGGATGAGCTAC
CAGTACCCGTGAAATTGGTGCATCTCAATAAATGCCCTATTCTCGCTCCGGCCAAAACCTTGACCGCAGAAAATGCACAA
AAGATTGGTATTGACCGCGAACAGTGTCTCGCGCACTTGAATGTGATTCGTCAACACCCCGAGATTCGTGAAAAGCTGGT
GACGGTATTTAGCCAAGAGCGCGAATTTAACCAAGAGAGTGATGTGGATAGCCAACTCTATGCCGGCTTTTTCTCCCCAG
CCGATCGCGCGGCGATGGAGATCATCCGCACCACTGCACCGGAGAATCTGGGCAGCTTAGCGATCCGGGTGGCCGATAAG
CGCATTGAACCGCTGCTGTTTCGTTATCGTGCGCGCCATTATCCCCACACCTTAACCGATACCGAGCAACGCCGCTGGGC
AGCGCATTGTCGTGACTATTTCGAGCGCAATCTAGAAGGTTATATGTTGAATTTGGAAAATCTGGTGCATGAACATCAAG
CTGATCCAAAGAAAATGGCGATTTTAAAATCCGTCTACCATTACGTGGAAAAACTCGCTTGCTAA

Domains

Predicted by InterproScan.

Exonuclease C-terminal (Exonuc_X-T_C)

(210-470)

Exonuclease (RNase_T)

(11-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3AJR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Triana N Dalia et al. (2025) SbcB facilitates natural transformation in Vibrio cholerae in an exonuclease-independent manner. Journal of Bacteriology 207(1):e0041924. [PMID: 39670763]