Detailed information    

experimental Experimentally validated

Overview

Name   recA   Type   Machinery gene
Locus tag   NCTC11637_00359 Genome accession   LS483488
Coordinates   365357..366400 (+) Length   347 a.a.
NCBI ID   SQJ03206.1    Uniprot ID   -
Organism   Helicobacter pylori strain NCTC11637     
Function   homologous recombination   
Homologous recombination

Function

Compared to the wild-type strains, isogenic H. pylori recA deletion mutants displayed increased sensitivity to ultraviolet light and abolished general homologous recombination.


Genomic Context

Location: 360357..371400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCTC11637_00351 - 360947..361165 (+) 219 SQJ03188.1 cb-type cytochrome c oxidase subunit IV -
  NCTC11637_00352 fixP 361167..362045 (+) 879 SQJ03190.1 cb-type cytochrome c oxidase subunit III -
  NCTC11637_00353 - 362056..362262 (+) 207 SQJ03192.1 Uncharacterised protein -
  NCTC11637_00354 - 362259..362366 (+) 108 SQJ03194.1 Uncharacterised protein -
  NCTC11637_00355 - 362363..362947 (+) 585 SQJ03199.1 putative inner membrane protein -
  NCTC11637_00356 - 362959..363540 (+) 582 SQJ03201.1 putative inner membrane protein -
  NCTC11637_00357 - 363628..364395 (+) 768 SQJ03203.1 membrane protein -
  NCTC11637_00358 - 364392..365258 (-) 867 SQJ03205.1 succinyl-CoA ligase -
  NCTC11637_00359 recA 365357..366400 (+) 1044 SQJ03206.1 recombinase A Machinery gene
  NCTC11637_00360 eno 366412..367692 (+) 1281 SQJ03208.1 phosphopyruvate hydratase -
  NCTC11637_00361 - 367685..367960 (+) 276 SQJ03210.1 Uncharacterised protein -
  NCTC11637_00362 - 367978..368574 (+) 597 SQJ03212.1 Putative periplasmic protein -
  NCTC11637_00363 aroK 368579..369067 (+) 489 SQJ03213.1 shikimate kinase -
  NCTC11637_00364 pglG 369089..370045 (+) 957 SQJ03215.1 N-linked glycosylation glycosyltransferase -
  NCTC11637_00365 gspA_1 370042..371160 (-) 1119 SQJ03217.1 lipopolysaccharide 1,2-glucosyltransferase RfaJ -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37654.39 Da        Isoelectric Point: 5.5047

>NTDB_id=1214 NCTC11637_00359 SQJ03206.1 365357..366400(+) (recA) [Helicobacter pylori strain NCTC11637]
MAIDEDKQKAISLAIKQIDKVFGKGALVRLGDKQVEKIDAISTGSLGLDLALGIGGVPKGRIIEIYGPESSGKTTLSLHI
IAECQKNGGVCAFIDAEHALDVHYAKRLGVDTENLLVSQPDTGEQALEILETITRSGGIDLVVVDSVAALTPKAEIDGDM
GDQHVGLQARLMSHALRKITGVLHKMNTTLIFINQIRMKIGMMGYGSPETTTGGNALKFYASVRIDIRRIAALKQNEQHI
GNRAKAKVVKNKVAPPFREAEFDIMFGEGISKEGEIIDYGVKLDIVDKSGAWLSYQDKKLGQGRENAKALLKEDKALADE
ITLKIKESIGSNEEIMPLPDEPLEEME

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1214 NCTC11637_00359 SQJ03206.1 365357..366400(+) (recA) [Helicobacter pylori strain NCTC11637]
ATGGCAATAGATGAAGACAAACAAAAAGCGATTTCTTTAGCGATCAAACAAATTGATAAGGTTTTTGGTAAGGGGGCGTT
GGTGCGCCTTGGGGATAAGCAAGTAGAAAAGATTGACGCTATTTCTACAGGCTCGTTAGGGTTGGATCTGGCTTTAGGGA
TTGGGGGCGTTCCAAAGGGCAGGATCATTGAAATTTATGGGCCAGAGTCAAGCGGGAAGACCACTTTAAGCTTGCATATT
ATTGCAGAATGCCAAAAAAATGGCGGCGTGTGCGCGTTCATTGACGCTGAGCATGCCCTAGATGTGCATTACGCTAAGAG
ACTAGGCGTGGATACGGAAAACTTACTCGTTTCCCAACCTGATACAGGCGAGCAAGCTTTAGAGATTTTAGAAACGATCA
CAAGAAGCGGAGGGATTGATTTAGTGGTGGTGGATTCGGTGGCGGCTCTTACGCCTAAAGCGGAGATTGATGGGGATATG
GGCGATCAGCATGTGGGCTTGCAAGCAAGGCTTATGAGCCATGCGTTAAGAAAAATCACCGGTGTCTTGCACAAGATGAA
CACTACTCTTATTTTTATCAATCAAATCAGAATGAAGATTGGCATGATGGGTTATGGGAGTCCAGAGACCACAACCGGAG
GTAACGCTTTAAAATTCTATGCGAGCGTTAGGATTGATATTAGAAGAATCGCGGCTTTAAAGCAAAACGAACAGCATATT
GGCAACAGGGCTAAAGCCAAAGTGGTTAAAAATAAAGTCGCTCCACCCTTTAGAGAAGCGGAATTTGACATCATGTTTGG
GGAGGGGATTTCTAAAGAGGGCGAAATCATTGATTATGGCGTGAAATTAGACATTGTGGATAAGAGTGGGGCATGGCTTA
GCTACCAGGATAAAAAGCTAGGGCAAGGCCGAGAAAACGCTAAAGCCTTACTGAAAGAAGACAAAGCCCTAGCGGATGAA
ATCACTCTTAAGATTAAAGAGAGTATTGGCTCTAATGAAGAGATCATGCCCTTACCCGATGAGCCTTTAGAAGAAATGGA
ATAA

Domains

Predicted by InterproScan.

recA bacterial DNA recombination protein (RecA)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Helicobacter pylori 26695

99.424

100

0.994
  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

78.963

95.627

0.755
  recA Neisseria gonorrhoeae MS11

66.769

93.66

0.625
  recA Neisseria gonorrhoeae MS11

66.769

93.66

0.625
  recA Neisseria gonorrhoeae strain FA1090

66.769

93.66

0.625
  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

64.458

96.793

0.624
  recA Bacillus subtilis subsp. subtilis str. 168

63.081

98.851

0.624
  recA Acinetobacter baumannii D1279779

64.22

94.236

0.605
  recA Acinetobacter baylyi ADP1

63.914

94.236

0.602
  recA Ralstonia pseudosolanacearum GMI1000

61.905

96.83

0.599
  recA Vibrio cholerae strain A1552

61.31

96.83

0.594
  recA Vibrio cholerae O1 biovar El Tor strain E7946

61.31

96.83

0.594
  recA Pseudomonas stutzeri DSM 10701

60.234

98.559

0.594
  recA Glaesserella parasuis strain SC1401

60.671

94.524

0.573
  recA Streptococcus pneumoniae Rx1

56.125

100

0.568
  recA Streptococcus pneumoniae D39

56.125

100

0.568
  recA Streptococcus pneumoniae R6

56.125

100

0.568
  recA Streptococcus pneumoniae TIGR4

56.125

100

0.568
  recA Latilactobacillus sakei subsp. sakei 23K

54.902

100

0.565
  recA Lactococcus lactis subsp. cremoris KW2

58.055

94.813

0.55
  recA Streptococcus pyogenes NZ131

57.879

95.101

0.55
  recA Streptococcus mitis NCTC 12261

57.751

94.813

0.548
  recA Streptococcus mitis SK321

57.447

94.813

0.545
  recA Streptococcus mutans UA159

56.287

96.254

0.542
  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.442

93.948

0.53

Multiple sequence alignment    



References

[1] W Schmitt et al. (1995) Cloning of the Helicobacter pylori recA gene and functional characterization of its product. Molecular & General Genetics : MGG 248(5):563-72. [PMID: 7476856]