Detailed information NTG0001313

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: DR_RS12030
• Genome accession: NC_001263
• Coordinates: 2337795..2338886 (+)
• Length: 363 a.a.
• NCBI ID: WP_010888966.1
• Uniprot ID: P42443
• Organism: Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539
• Function: homologous recombination Homologous recombination

Function

RecA is essential for chromosomal transformation but not for plasmid establishment.

Genomic Context

Location: 2332795..2343886

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DR_RS12005 (DR_2335)	-	2333447..2334127 (-)	681	WP_010888961.1	DsbA family oxidoreductase	-
DR_RS12010 (DR_2336)	-	2334286..2335248 (-)	963	WP_010888962.1	potassium channel family protein	-
DR_RS12015 (DR_2337)	-	2335245..2335742 (-)	498	WP_010888963.1	hypothetical protein	-
DR_RS12020 (DR_2338)	-	2335883..2337097 (+)	1215	WP_010888964.1	CinA family nicotinamide mononucleotide deamidase-related protein	-
DR_RS12025 (DR_2339)	thpR	2337094..2337798 (+)	705	WP_010888965.1	RNA 2',3'-cyclic phosphodiesterase	-
DR_RS12030 (DR_2340)	recA	2337795..2338886 (+)	1092	WP_010888966.1	recombinase RecA	Machinery gene
DR_RS12035	-	2339068..2339988 (-)	921	WP_028328034.1	bifunctional biotin--[acetyl-CoA-carboxylase] synthetase/biotin operon repressor	-
DR_RS12040	-	2340095..2340489 (+)	395	Protein_2371	transcriptional repressor	-
DR_RS12045 (DR_2342)	-	2340539..2341201 (+)	663	WP_010888968.1	hypothetical protein	-
DR_RS12055 (DR_2343)	-	2341403..2342056 (+)	654	WP_010888969.1	MarC family protein	-
DR_RS12060 (DR_2344)	-	2342113..2342595 (-)	483	WP_034350554.1	hypothetical protein	-
DR_RS12065 (DR_2345)	-	2342780..2343697 (-)	918	WP_010888971.1	metallophosphoesterase	-

Sequence

Protein

• Length: 363 a.a.
• Molecular weight: 38144.76 Da
• Isoelectric Point: 5.1867

>NTDB_id=1313 DR_RS12030 WP_010888966.1 2337795..2338886(+) (recA) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
MSKDATKEISAPTDAKERSKAIETAMSQIEKAFGKGSIMKLGAESKLDVQVVSTGSLSLDLALGVGGIPRGRITEIYGPE
SGGKTTLALAIVAQAQKAGGTCAFIDAEHALDPVYARALGVNTDELLVSQPDNGEQALEIMELLVRSGAIDVVVVDSVAA
LTPRAEIEGDMGDSLPGLQARLMSQALRKLTAILSKTGTAAIFINQVREKIGVMYGNPETTTGGRALKFYASVRLDVRKI
GQPTKVGNDAVANTVKIKTVKNKVAAPFKEVELALVYGKGFDQLSDLVGLAADMDIIKKAGSFYSYGDERIGQGKEKTIA
YIAERPEMEQEIRDRVMAAIRAGNAGEAPALAPAPAAPEAAEA

Nucleotide

• Length: 1092 bp

>NTDB_id=1313 DR_RS12030 WP_010888966.1 2337795..2338886(+) (recA) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
ATGAGCAAGGACGCCACCAAAGAAATCTCCGCCCCCACCGACGCCAAGGAACGCAGCAAGGCCATCGAAACAGCCATGAG
CCAGATCGAAAAGGCCTTCGGCAAGGGCTCGATCATGAAACTGGGCGCCGAGAGCAAACTCGACGTGCAGGTCGTCAGCA
CCGGCAGCCTCAGCCTTGACCTCGCACTGGGCGTGGGCGGCATTCCGCGTGGCCGCATCACCGAGATCTACGGCCCCGAG
TCGGGCGGCAAGACCACCCTGGCCCTCGCCATCGTCGCGCAGGCCCAGAAAGCGGGCGGCACCTGTGCGTTTATCGACGC
CGAGCACGCGCTCGACCCGGTGTACGCCCGCGCCCTGGGCGTGAACACCGACGAACTGCTGGTGTCGCAGCCCGACAACG
GCGAGCAGGCGCTCGAAATCATGGAACTGCTGGTGCGTTCGGGCGCGATTGATGTGGTGGTCGTGGACTCGGTGGCTGCT
CTGACCCCCCGCGCCGAAATCGAGGGCGACATGGGCGACAGCCTGCCCGGTCTTCAGGCCCGCCTGATGTCGCAGGCGCT
GCGCAAGCTGACGGCGATTCTCTCCAAGACCGGCACCGCCGCCATCTTCATCAACCAGGTTCGCGAGAAAATCGGCGTGA
TGTACGGCAACCCCGAAACCACCACCGGGGGCCGGGCGCTGAAGTTCTACGCCAGCGTGCGCCTCGACGTGCGTAAGATC
GGCCAGCCCACCAAGGTCGGCAACGACGCGGTCGCCAACACCGTCAAGATCAAGACCGTGAAGAACAAGGTCGCCGCCCC
CTTCAAGGAAGTCGAACTCGCGCTGGTCTACGGCAAGGGCTTCGACCAGCTCAGCGACCTCGTGGGCCTGGCCGCCGACA
TGGACATCATCAAGAAGGCCGGCAGCTTCTACTCCTACGGCGACGAGCGCATCGGCCAGGGCAAGGAAAAGACCATCGCC
TACATCGCCGAGCGCCCCGAGATGGAGCAGGAAATCCGCGACCGCGTGATGGCCGCCATCCGCGCGGGCAACGCGGGCGA
AGCACCGGCCCTGGCCCCCGCGCCTGCCGCGCCCGAAGCCGCCGAAGCGTAA

Domains

Predicted by InterProScan.

• recA bacterial DNA recombination protein (RecA_N) (20-281)

3D structure

• PDB: 1XP8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Acinetobacter baumannii D1279779	62.883	93.41	0.587
recA	Acinetobacter nosocomialis M2	62.577	93.143	0.583
recA	Acinetobacter baylyi ADP1	63.81	90.258	0.576
recA	Streptococcus pneumoniae D39	64.706	88.981	0.576
recA	Streptococcus pneumoniae R36A	64.706	88.981	0.576
recA	Streptococcus pneumoniae Rx1	64.706	88.981	0.576
recA	Streptococcus pneumoniae R6	64.706	88.981	0.576
recA	Streptococcus pneumoniae TIGR4	64.706	88.981	0.576
recA	Streptococcus mitis NCTC 12261	64.396	88.981	0.573
recA	Streptococcus mitis SK321	64.396	88.981	0.573
recA	Glaesserella parasuis strain SC1401	64.396	88.981	0.573
recA	Neisseria gonorrhoeae strain FA1090	60.121	95.115	0.572
recA	Neisseria gonorrhoeae MS11	60.121	95.115	0.572
recA	Bacillus subtilis subsp. subtilis str. 168	60.681	92.816	0.563
recA	Staphylococcus aureus strain ATCC 12600	61.129	91.931	0.562
recA	Latilactobacillus sakei subsp. sakei 23K	61.61	90.986	0.561
recA	Ralstonia pseudosolanacearum GMI1000	60.123	92.614	0.557
recA	Streptococcus thermophilus LMD-9	62.539	88.981	0.556
recA	Streptococcus thermophilus LMG 18311	62.539	88.981	0.556
recA	Streptococcus pyogenes NZ131	62.229	88.981	0.554
recA	Streptococcus mutans UA159	61.92	88.981	0.551
recA	Vibrio cholerae O1 biovar El Tor strain E7946	62.3	88.418	0.551
recA	Vibrio cholerae strain A1552	62.3	88.418	0.551
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	54.81	100	0.548
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	59.177	92.128	0.545
recA	Lactococcus lactis subsp. cremoris KW2	57.817	93.388	0.54
recA	Helicobacter pylori 26695	56.442	93.948	0.53
recA	Helicobacter pylori strain NCTC11637	56.442	93.948	0.53
recA	Pseudomonas stutzeri DSM 10701	56	93.66	0.524

References

• [1]: Solenne Ithurbide et al. (2020) Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals the Major Roles of DprA, DdrB, RecA, RecF, and RecO Proteins. Frontiers in Microbiology 11:1253. [PMID: 32625182]
• [2]: Yuan Wang et al. (2019) Distinct roles of Deinococcus radiodurans RecFOR and RecA in DNA transformation. Biochemical And Biophysical Research Communications 513(3):740-745. [PMID: 30992133]
• [3]: J D Carroll et al. (1996) Expression of recA in Deinococcus radiodurans. Journal of Bacteriology 178(1):130-5. [PMID: 8550406]