Detailed information NTG0001022

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: ACIAD_RS06390
• Genome accession: NC_005966
• Coordinates: 1380692..1381741 (-)
• Length: 349 a.a.
• NCBI ID: WP_004925639.1
• Uniprot ID: A0A0A8JAA4
• Organism: Acinetobacter baylyi ADP1
• Function: homologous recombination Homologous recombination

Function

RecA protein is essential for the formation of a circular, double-stranded cytoplasmic intermediate from incoming donor DNA.

Genomic Context

Location: 1375692..1386741

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACIAD_RS06360 (ACIAD1379)	-	1376005..1376505 (+)	501	WP_004925653.1	OmpH family outer membrane protein	-
ACIAD_RS06365 (ACIAD1380)	lpxD	1376509..1377579 (+)	1071	WP_011182273.1	UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase	-
ACIAD_RS06370 (ACIAD1381)	fabZ	1377586..1378071 (+)	486	WP_026056961.1	3-hydroxyacyl-ACP dehydratase FabZ	-
ACIAD_RS06375 (ACIAD1382)	lpxA	1378068..1378856 (+)	789	WP_004925646.1	acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase	-
ACIAD_RS06380 (ACIAD1383)	-	1378916..1379743 (-)	828	WP_004925645.1	YbgF trimerization domain-containing protein	-
ACIAD_RS06385 (ACIAD1384)	-	1380155..1380634 (-)	480	WP_004925642.1	regulatory protein RecX	-
ACIAD_RS06390 (ACIAD1385)	recA	1380692..1381741 (-)	1050	WP_004925639.1	recombinase RecA	Machinery gene
ACIAD_RS06395 (ACIAD1386)	-	1381899..1382342 (-)	444	WP_004925636.1	RNA-binding S4 domain-containing protein	-
ACIAD_RS06400 (ACIAD1387)	-	1382955..1383815 (+)	861	WP_004925631.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
ACIAD_RS06405 (ACIAD1388)	-	1383872..1384606 (+)	735	WP_004925629.1	phosphoglycerate mutase family protein	-
ACIAD_RS06410 (ACIAD1389)	rpsT	1384664..1384933 (-)	270	WP_004925626.1	30S ribosomal protein S20	-
ACIAD_RS06415 (ACIAD1390)	-	1385143..1385805 (+)	663	WP_004925623.1	nucleoside-diphosphate sugar epimerase	-
ACIAD_RS06420 (ACIAD1391)	rraA	1385821..1386333 (+)	513	WP_004925622.1	ribonuclease E activity regulator RraA	-

Sequence

Protein

• Length: 349 a.a.
• Molecular weight: 37815.20 Da
• Isoelectric Point: 5.0237

>NTDB_id=1022 ACIAD_RS06390 WP_004925639.1 1380692..1381741(-) (recA) [Acinetobacter baylyi ADP1]
MDDNKSKALQAALSQIEKQFGKNTVMRLGDNTVQAVEAVSTGSLTLDIALGIGGLPKGRIVEIYGPESSGKTTMTLQAIA
QCQKAGGTCAFIDAEHALDPQYARKLGVDIDNLLVSQPDHGEQALEIADMLVRSGAIDLIVVDSVAALTPKAEIEGEMGD
SHMGLQARLMSQALRKITGNAKRSNCMVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGQVKEGDEIIGSE
TKVKVVKNKMAPPFREAIFQILYGKGVNQLGELVDLAVQQNIVQKAGAWYSYQGNKIGQGKNNVIRYLEENPQISTEIEA
VIREQLLTKASDQTAAHDETEEEPDLLES

Nucleotide

• Length: 1050 bp

>NTDB_id=1022 ACIAD_RS06390 WP_004925639.1 1380692..1381741(-) (recA) [Acinetobacter baylyi ADP1]
ATGGATGATAATAAAAGCAAGGCATTACAAGCTGCTTTAAGTCAGATTGAAAAACAATTTGGCAAAAATACAGTAATGCG
TTTAGGTGATAATACCGTTCAAGCTGTTGAAGCCGTTTCGACAGGCTCTCTTACTCTAGATATTGCATTAGGTATTGGTG
GCTTACCTAAAGGGCGTATTGTAGAGATTTACGGTCCTGAATCTTCTGGTAAAACAACCATGACTTTGCAAGCAATTGCA
CAATGTCAGAAAGCAGGTGGTACCTGTGCTTTTATTGATGCAGAACATGCATTAGACCCTCAGTATGCCCGTAAGCTGGG
TGTAGACATAGACAATCTTTTGGTATCGCAGCCTGATCATGGTGAACAAGCTCTGGAAATTGCAGATATGCTTGTACGCT
CTGGTGCGATTGATCTGATTGTAGTCGACTCTGTAGCAGCATTAACCCCAAAAGCCGAAATTGAAGGTGAAATGGGCGAC
TCCCACATGGGTTTACAAGCCCGCTTAATGAGTCAAGCCTTACGTAAAATCACGGGTAATGCTAAACGTTCGAATTGCAT
GGTAATCTTCATTAACCAGATTCGTATGAAAATTGGCGTAATGTTTGGCAGCCCAGAAACCACAACTGGTGGTAATGCAC
TAAAATTCTATGCATCTGTACGTCTAGATATACGCCGTATTGGTCAAGTTAAAGAAGGTGATGAAATCATTGGTTCTGAA
ACCAAAGTCAAAGTCGTTAAAAATAAAATGGCCCCTCCTTTCCGTGAAGCAATCTTCCAAATTTTATATGGCAAGGGTGT
CAATCAACTTGGCGAATTGGTTGATCTTGCAGTTCAACAAAATATTGTACAAAAAGCTGGTGCTTGGTATTCATACCAAG
GCAATAAAATTGGTCAGGGTAAGAATAACGTGATTCGTTATCTTGAAGAGAACCCTCAGATTTCTACAGAAATTGAAGCG
GTTATCCGTGAACAATTATTGACTAAGGCATCAGATCAGACCGCTGCTCACGATGAAACTGAAGAAGAACCAGATTTACT
GGAAAGTTAA

Domains

Predicted by InterproScan.

• recA bacterial DNA recombination protein (RecA) (7-268)

3D structure

• AlphaFold DB: A0A0A8JAA4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Acinetobacter baumannii D1279779	93.084	99.427	0.926
recA	Pseudomonas stutzeri DSM 10701	75.224	96.542	0.726
recA	Vibrio cholerae O1 biovar El Tor strain E7946	70.69	99.713	0.705
recA	Vibrio cholerae strain A1552	70.69	99.713	0.705
recA	Ralstonia pseudosolanacearum GMI1000	67.33	100	0.679
recA	Glaesserella parasuis strain SC1401	69.298	97.994	0.679
recA	Neisseria gonorrhoeae strain FA1090	66.859	99.713	0.667
recA	Neisseria gonorrhoeae MS11	66.859	99.713	0.667
recA	Neisseria gonorrhoeae MS11	66.859	99.713	0.667
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	62.974	100	0.63
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	63.772	97.376	0.621
recA	Helicobacter pylori strain NCTC11637	63.914	94.236	0.602
recA	Helicobacter pylori 26695	63.609	94.236	0.599
recA	Streptococcus mutans UA159	59.375	100	0.599
recA	Streptococcus mitis NCTC 12261	58.192	100	0.59
recA	Streptococcus pyogenes NZ131	62.154	93.123	0.579
recA	Bacillus subtilis subsp. subtilis str. 168	62.422	92.529	0.578
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	63.81	90.258	0.576
recA	Streptococcus pneumoniae Rx1	61.35	93.41	0.573
recA	Streptococcus pneumoniae D39	61.35	93.41	0.573
recA	Streptococcus pneumoniae R6	61.35	93.41	0.573
recA	Streptococcus pneumoniae TIGR4	61.35	93.41	0.573
recA	Streptococcus mitis SK321	61.043	93.41	0.57
recA	Lactococcus lactis subsp. cremoris KW2	59.443	92.55	0.55
recA	Latilactobacillus sakei subsp. sakei 23K	59.19	91.977	0.544

References

• [1]: Julia Kloos et al. (2021) Tn1 transposition in the course of natural transformation enables horizontal antibiotic resistance spread in Acinetobacter baylyi. Microbiology (Reading, England) 167(1):001003. [PMID: 33270000]