Detailed information    

experimental Experimentally validated

Overview

Name   recA   Type   Machinery gene
Locus tag   OK783_RS03845 Genome accession   NZ_CP115654
Coordinates   736929..737975 (+) Length   348 a.a.
NCBI ID   WP_003688695.1    Uniprot ID   Q5F8M9
Organism   Neisseria gonorrhoeae strain FA1090     
Function   homologous recombination   
Homologous recombination

Function

DprA mutants exhibited increased RecA-dependent pilin variation suggesting that DprA affects pilin variation. Unlike the exquisite UV sensitivity of a recA mutant, inactivation of dprA did not affect survival following UV irradiation.


Genomic Context

Location: 731929..742975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OK783_RS03830 (OK783_03830) dinB 732802..733860 (+) 1059 WP_003695315.1 DNA polymerase IV -
  OK783_RS03835 (OK783_03835) rep 733930..735945 (-) 2016 WP_003706226.1 DNA helicase Rep -
  OK783_RS03840 (OK783_03840) aroD 735965..736729 (-) 765 WP_003688696.1 type I 3-dehydroquinate dehydratase -
  OK783_RS03845 (OK783_03845) recA 736929..737975 (+) 1047 WP_003688695.1 recombinase RecA Machinery gene
  OK783_RS03850 (OK783_03850) - 738628..738963 (-) 336 WP_003688693.1 YbaB/EbfC family nucleoid-associated protein -
  OK783_RS03855 (OK783_03855) dnaX 739044..741167 (-) 2124 WP_003695312.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 37642.87 Da        Isoelectric Point: 4.8330

>NTDB_id=1123 OK783_RS03845 WP_003688695.1 736929..737975(+) (recA) [Neisseria gonorrhoeae strain FA1090]
MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGSLGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAV
AQCQKNGGVCAFVDAEHAFDPVYARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFYSSVRLDIRRTGSIKKGEEVLGN
ETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELIDIGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEID
AKIRALNGVEMHITEGTQDETDGERPEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=1123 OK783_RS03845 WP_003688695.1 736929..737975(+) (recA) [Neisseria gonorrhoeae strain FA1090]
ATGTCAGACGACAAAAGCAAAGCCCTTGCCGCCGCACTGGCGCAAATCGAAAAAAGTTTCGGCAAAGGCGCCATCATGAA
AATGGACGGCAGCCAGCAGGAAGAAAACCTCGAAGTCATTTCCACCGGATCGCTCGGATTAGACCTCGCCCTCGGAGTCG
GCGGCCTGCCGCGCGGGCGCATCGTCGAAATCTTCGGCCCCGAATCCTCCGGCAAAACCACACTCTGCCTCGAAGCCGTC
GCCCAATGCCAGAAAAACGGCGGCGTGTGCGCCTTTGTCGATGCCGAACACGCCTTCGATCCCGTTTACGCCCGCAAACT
CGGCGTAAAAGTCGAAGAGCTTTACCTGTCCCAGCCCGATACCGGCGAACAGGCTTTGGAAATCTGCGACACGCTCGTCC
GTTCGGGCGGCATAGATATGGTAGTCGTCGATTCCGTAGCCGCACTCGTCCCCAAAGCCGAAATCGAAGGCGATATGGGG
GACAGCCACGTCGGACTGCAGGCGCGCCTGATGAGTCAGGCTTTGCGCAAACTGACCGGACACATCAAAAAAACCAATAC
GCTGGTTGTGTTCATCAACCAAATCCGAATGAAGATCGGCGTAATGTTCGGCAGCCCCGAGACCACGACCGGCGGCAACG
CGCTGAAATTCTATTCTTCCGTCCGCCTCGACATCCGCCGCACCGGATCCATCAAAAAAGGCGAAGAAGTATTGGGCAAC
GAAACCCGCGTCAAAGTCATCAAAAACAAAGTCGCCCCCCCGTTCCGTCAGGCAGAGTTTGACATCCTCTACGGAGAAGG
CATCAGTTGGGAAGGCGAATTGATCGACATCGGCGTGAAAAACGACATCATCAACAAATCCGGCGCGTGGTACAGCTACA
ACGGCGCGAAAATCGGTCAGGGCAAAGACAACGTCCGCGTCTGGCTGAAAGAAAACCCCGAAATTTCCGACGAAATCGAC
GCAAAAATCCGCGCCCTCAACGGCGTAGAAATGCACATCACCGAAGGGACGCAGGACGAAACCGACGGCGAACGACCCGA
AGAATAA

Domains

Predicted by InterProScan.

recA bacterial DNA recombination protein (RecA_N)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5F8M9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Neisseria gonorrhoeae MS11

100

100

1
  recA Ralstonia pseudosolanacearum GMI1000

74.468

94.54

0.704
  recA Vibrio cholerae O1 biovar El Tor strain E7946

66.949

100

0.681
  recA Vibrio cholerae strain A1552

66.949

100

0.681
  recA Glaesserella parasuis strain SC1401

67.521

100

0.681
  recA Pseudomonas stutzeri DSM 10701

71.692

93.66

0.671
  recA Acinetobacter baylyi ADP1

66.859

99.713

0.667
  recA Acinetobacter nosocomialis M2

65.812

100

0.664
  recA Acinetobacter baumannii D1279779

65.517

100

0.655
  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

65.103

99.417

0.647
  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

67.073

95.627

0.641
  recA Staphylococcus aureus strain ATCC 12600

65.192

97.695

0.637
  recA Bacillus subtilis subsp. subtilis str. 168

63.584

99.425

0.632
  recA Helicobacter pylori 26695

67.077

93.66

0.628
  recA Helicobacter pylori strain NCTC11637

66.769

93.66

0.625
  recA Streptococcus mutans UA159

60.684

100

0.612
  recA Streptococcus pneumoniae R36A

59.544

100

0.601
  recA Streptococcus pneumoniae Rx1

59.544

100

0.601
  recA Streptococcus pneumoniae D39

59.544

100

0.601
  recA Streptococcus pneumoniae R6

59.544

100

0.601
  recA Streptococcus pneumoniae TIGR4

59.544

100

0.601
  recA Streptococcus thermophilus LMD-9

59.827

99.425

0.595
  recA Streptococcus thermophilus LMG 18311

59.827

99.425

0.595
  recA Streptococcus mitis NCTC 12261

59.884

98.851

0.592
  recA Latilactobacillus sakei subsp. sakei 23K

62.691

93.966

0.589
  recA Streptococcus pyogenes NZ131

61.963

93.678

0.58
  recA Streptococcus mitis SK321

60.606

94.828

0.575
  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.121

95.115

0.572
  recA Lactococcus lactis subsp. cremoris KW2

60.303

94.828

0.572

Multiple sequence alignment    



References

[1] Paul M Duffin et al. (2016) DprA is required for natural transformation and affects pilin variation in Neisseria gonorrhoeae. Microbiology (Reading, England) 162(9):1620-1628. [PMID: 27469477]