Detailed information NTG0001165

Experimentally validated

Overview

• Name: recA
• Type: Machinery gene
• Locus tag: A1552VC_RS01565
• Genome accession: NZ_CP028894
• Coordinates: 302577..303641 (+)
• Length: 354 a.a.
• NCBI ID: WP_000344154.1
• Uniprot ID: A0A366AH98
• Organism: Vibrio cholerae strain A1552
• Function: homologous recombination Homologous recombination

Function

Once inside the cytoplasm, Ssb and DprA bind the ssDNA and facilitate loading of RecA, which ultimately catalyzes recombination with the bacterial chromosome.

Genomic Context

Location: 297577..308641

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A1552VC_RS01540 (A1552VC_00301)	cysP	297840..298841 (+)	1002	WP_001079835.1	thiosulfate ABC transporter substrate-binding protein CysP	-
A1552VC_RS01545 (A1552VC_00302)	cysT	298918..299769 (+)	852	WP_000531810.1	sulfate/thiosulfate ABC transporter permease CysT	-
A1552VC_RS01550 (A1552VC_00303)	cysW	299780..300643 (+)	864	WP_000951362.1	sulfate ABC transporter permease subunit CysW	-
A1552VC_RS01555 (A1552VC_00304)	-	300640..301770 (+)	1131	WP_000027776.1	sulfate/molybdate ABC transporter ATP-binding protein	-
A1552VC_RS01560 (A1552VC_00305)	pncC	301937..302443 (+)	507	WP_000853811.1	nicotinamide-nucleotide amidase	-
A1552VC_RS01565 (A1552VC_00306)	recA	302577..303641 (+)	1065	WP_000344154.1	recombinase RecA	Machinery gene
A1552VC_RS01570 (A1552VC_00307)	recX	303774..304232 (+)	459	WP_000006855.1	recombination regulator RecX	-
A1552VC_RS01575 (A1552VC_00308)	alaS	304405..306987 (+)	2583	WP_000481017.1	alanine--tRNA ligase	-
A1552VC_RS01580 (A1552VC_00309)	-	307191..308378 (+)	1188	WP_001883372.1	aspartate kinase	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38249.92 Da
• Isoelectric Point: 5.0766

>NTDB_id=1165 A1552VC_RS01565 WP_000344154.1 302577..303641(+) (recA) [Vibrio cholerae strain A1552]
MDENKQKALAAALGQIEKQFGKGSIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRIVEIFGPESSGKTTLTLELIA
AAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGD
SHMGLQARMLSQAMRKLTGNLKQSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNE
TRIKVVKNKIAAPFKEANTQIMYGQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQGKANACKYLKENPEIAKTLDK
KLREMLLNPENMQLIAETSSAADDVEFGAVPEEF

Nucleotide

• Length: 1065 bp

>NTDB_id=1165 A1552VC_RS01565 WP_000344154.1 302577..303641(+) (recA) [Vibrio cholerae strain A1552]
ATGGACGAGAATAAACAGAAGGCACTTGCGGCCGCCCTCGGTCAAATTGAAAAGCAATTTGGTAAAGGCTCCATCATGCG
CTTAGGCGACAACCGCGCGATGGATGTAGAAACCATTTCGACCGGTTCTCTGTCTCTGGATATCGCGTTGGGTGCTGGTG
GTCTACCAATGGGACGTATCGTTGAGATCTTCGGCCCTGAATCTTCGGGTAAAACGACACTGACTCTGGAACTGATTGCT
GCTGCACAACGTGAAGGTAAAACCTGTGCGTTTATCGATGCCGAGCACGCACTGGATCCGGTTTATGCCAAAAAATTGGG
CGTGAATATCGATGAGCTACTGGTTTCTCAGCCAGATACCGGCGAGCAAGCACTGGAAATTTGTGATGCACTGGCTCGCT
CTGGTGCTGTGGATGTCATCGTTGTTGACTCAGTAGCGGCTTTGACACCAAAAGCGGAAATTGAAGGCGAAATGGGCGAT
AGCCACATGGGTCTGCAAGCGCGTATGTTGTCGCAAGCAATGCGTAAACTGACGGGTAACCTCAAGCAATCCAACTGTAT
GTGTATCTTCATCAACCAAATTCGTATGAAGATTGGTGTGATGTTTGGTAACCCAGAAACTACCACTGGCGGTAACGCAC
TGAAATTCTACGCTTCTGTTCGTTTGGATATTCGCCGTACTGGCGCAATCAAAGAAGGCGAAGAAGTGGTGGGTAACGAA
ACCCGTATCAAGGTTGTGAAGAACAAGATTGCTGCGCCGTTTAAAGAAGCGAACACTCAGATCATGTATGGCCAAGGTTT
CAATCGTGAAGGTGAATTGATCGATCTTGGTGTGAAACACAAAATGGTTGAGAAGTCAGGCGCTTGGTACAGCTATAACG
GCGATAAAATCGGCCAAGGTAAAGCGAATGCCTGCAAGTATCTGAAAGAAAACCCAGAAATTGCGAAAACTCTAGATAAA
AAACTGCGTGAAATGCTGTTAAACCCAGAAAACATGCAGCTGATCGCTGAAACATCTTCTGCGGCGGATGACGTCGAATT
TGGCGCGGTGCCAGAAGAGTTTTAA

Domains

Predicted by InterProScan.

• recA bacterial DNA recombination protein (RecA_N) (7-267)

3D structure

• AlphaFold DB: A0A366AH98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recA	Vibrio cholerae O1 biovar El Tor strain E7946	100	100	1
recA	Pseudomonas stutzeri DSM 10701	75.841	94.236	0.715
recA	Acinetobacter baylyi ADP1	70.69	99.713	0.705
recA	Acinetobacter baumannii D1279779	73.7	93.696	0.691
recA	Glaesserella parasuis strain SC1401	69.516	99.153	0.689
recA	Acinetobacter nosocomialis M2	73.394	93.429	0.686
recA	Neisseria gonorrhoeae strain FA1090	66.949	100	0.681
recA	Neisseria gonorrhoeae MS11	66.949	100	0.681
recA	Ralstonia pseudosolanacearum GMI1000	69.822	96.023	0.67
recA	Staphylococcus aureus strain ATCC 12600	65.644	93.948	0.617
recA	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	64.506	94.461	0.609
recA	Bacillus subtilis subsp. subtilis str. 168	60.465	98.851	0.598
recA	Helicobacter pylori strain NCTC11637	61.31	96.83	0.594
recA	Helicobacter pylori 26695	61.012	96.83	0.591
recA	Riemerella anatipestifer ATCC 11845 = DSM 15868	61.027	96.501	0.589
recA	Streptococcus pneumoniae D39	62.848	91.243	0.573
recA	Streptococcus pneumoniae R36A	62.848	91.243	0.573
recA	Streptococcus pneumoniae Rx1	62.848	91.243	0.573
recA	Streptococcus pneumoniae R6	62.848	91.243	0.573
recA	Streptococcus pneumoniae TIGR4	62.848	91.243	0.573
recA	Streptococcus mitis NCTC 12261	62.229	91.243	0.568
recA	Streptococcus mitis SK321	61.92	91.243	0.565
recA	Streptococcus mutans UA159	61.231	91.808	0.562
recA	Streptococcus thermophilus LMG 18311	61.231	91.808	0.562
recA	Streptococcus thermophilus LMD-9	61.231	91.808	0.562
recA	Lactococcus lactis subsp. cremoris KW2	60.991	91.243	0.556
recA	Streptococcus pyogenes NZ131	60.615	91.808	0.556
recA	Latilactobacillus sakei subsp. sakei 23K	60.748	90.678	0.551
recA	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	62.3	88.418	0.551

References

• [1]: Patrick Seitz et al. (2013) DNA-uptake machinery of naturally competent Vibrio cholerae. Proceedings of The National Academy of Sciences of The United States of America 110(44):17987-92. [PMID: 24127573]
• [2]: Gaia Suckow et al. (2011) Quorum sensing contributes to natural transformation of Vibrio cholerae in a species-specific manner. Journal of Bacteriology 193(18):4914-24. [PMID: 21784943]