Detailed information NTG0000494

Experimentally validated

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: SM12261_RS04305
• Genome accession: NZ_CP028414
• Coordinates: 795536..797776 (+)
• Length: 746 a.a.
• NCBI ID: WP_000942409.1
• Uniprot ID: -
• Organism: Streptococcus mitis NCTC 12261
• Function: ssDNA transport into the cell DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 790536..802776

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SM12261_RS04275 (SM12261_0830)	cvfB	790767..791621 (+)	855	WP_001095458.1	RNA-binding virulence regulatory protein CvfB	-
SM12261_RS04280 (SM12261_0831)	-	791630..791845 (+)	216	WP_001232083.1	YozE family protein	-
SM12261_RS04285 (SM12261_0832)	-	791930..792925 (+)	996	WP_000658173.1	PhoH family protein	-
SM12261_RS04290 (SM12261_0833)	ald	792982..794094 (-)	1113	WP_000904719.1	alanine dehydrogenase	-
SM12261_RS04295 (SM12261_0834)	-	794266..794835 (+)	570	WP_000443732.1	GNAT family N-acetyltransferase	-
SM12261_RS04300 (SM12261_0835)	comEA/celA/cilE	794902..795552 (+)	651	WP_000387351.1	helix-hairpin-helix domain-containing protein	Machinery gene
SM12261_RS04305 (SM12261_0836)	comEC/celB	795536..797776 (+)	2241	WP_000942409.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SM12261_RS04310 (SM12261_0837)	infC	797996..798526 (+)	531	WP_000848180.1	translation initiation factor IF-3	-
SM12261_RS04315 (SM12261_0838)	rpmI	798559..798759 (+)	201	WP_001125943.1	50S ribosomal protein L35	-
SM12261_RS04320 (SM12261_0839)	rplT	798811..799170 (+)	360	WP_000124834.1	50S ribosomal protein L20	-
SM12261_RS04325 (SM12261_0840)	-	799346..801010 (+)	1665	WP_000635031.1	molecular chaperone HscC	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84460.75 Da
• Isoelectric Point: 9.5655

>NTDB_id=494 SM12261_RS04305 WP_000942409.1 795536..797776(+) (comEC/celB) [Streptococcus mitis NCTC 12261]
MLQWIKNFSIPLIYLSFLLLWLYYAIFSSSYLALLGFVFLLVCLFIQFPWKSAGKVLIICGIFGFWFLFQNWQQSQASQN
LADSVERVRILPDTVKVNGDSLSFRGKSDGRIFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPDGQRNFGGFDYQAY
LKTQGIYQTLTIKRIQSLQKVSSWDIGENLSSLRRKTVVWIKTHFPDPMRNYMTGLLLGHLDTDFEEMNGLYSSLGIIHL
FALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIV
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVARESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLVFLPLLSI
LFALSFLHPVIQLNFIFEWLEGMIRFVSQVASRPLVFGQPNAWILILLLISLALVYDLRKNIKRLAVLSLLITGLFFLTK
HPLENEITMLDVGQGESIFLRDVTGKIILIDVGGKAESSKKIEKWQEKTTTSNAQRTLIPYLKSRGVSKIDQLILTNTDK
EYVGDLLEVTKAFHVGEILVSKGSLKQKQFVAELQATQTKVRSITAGENLSIFGSQLEVLSPRKIGDGGYEDSLVLYGKL
LDKYFLFTGNLEEKGEKELLKHYPDLKVDVLKAGQHGSKKSSSSDFLEKLKPEFTLISVGKNNRAKLPHQETLTRLETIN
SKVYRTDQHGAIRFKGWNSWKVETVG

Nucleotide

• Length: 2241 bp

>NTDB_id=494 SM12261_RS04305 WP_000942409.1 795536..797776(+) (comEC/celB) [Streptococcus mitis NCTC 12261]
ATGTTACAGTGGATTAAGAATTTCTCTATTCCCCTAATTTACCTTAGTTTTCTGTTGCTTTGGCTTTACTACGCCATTTT
CTCATCATCTTATCTCGCACTACTAGGTTTTGTTTTTCTGCTAGTCTGTCTCTTTATCCAATTTCCGTGGAAATCTGCTG
GTAAAGTTCTAATAATTTGTGGAATCTTTGGTTTCTGGTTTCTGTTTCAAAATTGGCAACAGAGTCAAGCGAGTCAAAAT
TTGGCGGATTCTGTTGAAAGGGTACGGATTTTACCAGACACTGTTAAGGTCAATGGTGATAGTCTATCCTTTCGTGGTAA
GTCTGATGGACGCATTTTTCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAGCCTTTCAAGCTTTAACCGACC
TTCATGAGATAGGACTAGAAGGAAAACTTTCAGAACCAGACGGGCAGAGAAATTTTGGTGGTTTTGACTACCAAGCCTAT
CTGAAAACTCAAGGGATTTACCAAACATTAACTATCAAAAGAATCCAGTCACTTCAAAAGGTTAGCAGTTGGGATATAGG
AGAAAATCTGTCCAGTTTACGTCGAAAGACTGTGGTTTGGATTAAGACGCATTTTCCAGACCCTATGCGCAATTACATGA
CAGGGCTCTTGTTAGGACATCTGGACACCGACTTTGAGGAGATGAACGGGCTTTATTCTAGTTTAGGAATTATCCACCTC
TTTGCCTTGTCGGGTATGCAGGTAGGTTTTTTCATGGACGGATTTAAGAAACTCCTCTTGCGATTGGGCTTGACTCAAGA
AAAGTTGAAATGGCTGACTTATCCCTTTTCCCTTATCTATGCAGGGCTGACAGGATTTTCAGCATCAGTTATTCGCAGTC
TTTTGCAAAAGTTACTGGCACAACATGGTGTTAAGGGCTTGGATAATTTTGCCTTGACGGTCCTTGTCCTCTTTATCGTC
ATGCCCAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGCGCTTATGCTTTTATCTTGACCATGACCAGCAAAGAAGG
AGAAGGCCTCAAGGCTGTTGCTAGAGAAAGTCTAGTCATTTCCTTGGGAATATTACCCATCCTATCCTTCTATTTTGCAG
AATTTCAGCCTTGGTCTATCCTTTTGACCTTTGTCTTTTCCTTTCTTTTTGACTTGGTCTTCTTACCGCTCTTGTCTATC
TTATTTGCCCTTTCCTTTCTTCATCCAGTCATTCAGCTGAATTTTATTTTTGAATGGTTGGAGGGAATGATTCGCTTTGT
ATCACAGGTGGCAAGTAGACCTCTGGTCTTTGGACAACCCAATGCATGGATTTTAATCTTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGGAAAAACATTAAAAGGCTAGCAGTGTTGAGCTTATTGATTACAGGTCTCTTTTTCTTGACCAAG
CATCCACTGGAAAATGAAATCACCATGCTGGATGTTGGGCAAGGGGAAAGTATTTTCCTACGGGATGTAACTGGTAAAAT
CATTCTCATAGATGTGGGTGGCAAGGCAGAGTCTAGTAAGAAAATAGAAAAATGGCAAGAAAAGACGACGACCAGTAATG
CCCAGCGAACCTTGATACCCTATCTCAAAAGTCGCGGAGTATCCAAGATTGACCAGCTAATTTTGACCAATACGGACAAG
GAATATGTTGGAGATTTGTTGGAGGTGACCAAGGCTTTTCATGTAGGCGAAATTTTAGTGTCAAAAGGCAGTTTGAAACA
GAAGCAATTTGTGGCAGAACTACAAGCGACCCAAACAAAAGTTAGAAGTATAACAGCAGGAGAGAACTTGTCAATTTTTG
GAAGTCAGTTAGAAGTCCTATCTCCAAGGAAAATTGGAGATGGAGGTTATGAAGATTCCCTGGTTCTGTATGGAAAACTT
TTGGATAAGTACTTTCTCTTCACAGGAAATTTGGAGGAAAAAGGAGAGAAGGAATTGCTGAAGCACTATCCAGACTTGAA
AGTGGATGTTTTAAAAGCTGGCCAACATGGCTCTAAAAAATCATCAAGTTCAGACTTTCTAGAAAAACTCAAACCAGAGT
TTACTCTTATCTCAGTTGGAAAGAACAATCGAGCGAAACTCCCCCATCAGGAAACTTTGACACGACTGGAAACTATCAAT
AGTAAAGTTTACCGAACTGACCAGCATGGAGCTATACGCTTTAAAGGGTGGAATAGTTGGAAAGTCGAAACGGTTGGTTA
A

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (492-693)

• Domain of unknown function (DUF4131) (DUF4131) (29-176)

• Competence protein (Competence) (216-458)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis SK321	91.946	99.866	0.918
comEC/celB	Streptococcus pneumoniae TIGR4	91.812	99.866	0.917
comEC/celB	Streptococcus pneumoniae Rx1	91.544	99.866	0.914
comEC/celB	Streptococcus pneumoniae R6	91.544	99.866	0.914
comEC/celB	Streptococcus pneumoniae D39	91.544	99.866	0.914
comEC	Lactococcus lactis subsp. cremoris KW2	44.459	100	0.447

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]