Detailed information    

experimental Experimentally validated

Overview

Name   comS   Type   Regulator
Locus tag   - Genome accession   NZ_ACLO01000018
Coordinates   82608..82682 (-) Length   25 a.a.
NCBI ID   - Uniprot ID   -
Organism   Streptococcus salivarius SK126     
Function   activate transcription of comX; activate transcription of comS   
Competence regulation

Function

ComS is the precursor of the competence pheromone. ComS is secreted by as yet undiscovered transporter(s). At the cell surface, the protease Eep processes ComS, releasing the C-terminal pheromone domain named XIP (for σX inducing peptide). XIP interacts with the small chaperone protein AmiA3 and is brought to the Ami oligopeptide transporter. Once reimported, XIP then interacts with the transcriptional activator ComR and stimulates the binding of the ComR-XIP complex as a dimer to the ComR-box located in the promoter of comX and comS, thereby promoting their transcription. The ComR-XIP complex induces a positive feedback loop on ComS production, but not on ComR.


Genomic Context

Location: 77608..87682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STRSA0001_RS01265 (STRSA0001_1787) - 78596..79990 (+) 1395 WP_227826957.1 DUF4153 domain-containing protein -
  STRSA0001_RS01270 (STRSA0001_1788) - 80042..80374 (-) 333 WP_002883800.1 DUF805 domain-containing protein -
  STRSA0001_RS01275 (STRSA0001_1789) comA 80499..82595 (-) 2097 WP_002883724.1 peptide cleavage/export ABC transporter Regulator
  - comS 82608..82682 (-) 75 - - Regulator
  STRSA0001_RS01280 (STRSA0001_1790) comR 82770..83669 (-) 900 WP_002883753.1 helix-turn-helix transcriptional regulator Regulator
  STRSA0001_RS01285 (STRSA0001_1791) - 83849..84499 (-) 651 WP_002883719.1 phosphatase PAP2 family protein -
  STRSA0001_RS01290 (STRSA0001_1792) - 84502..85059 (-) 558 WP_002883726.1 ECF transporter S component -
  STRSA0001_RS01295 (STRSA0001_1794) - 85349..85858 (-) 510 WP_002883735.1 tRNA (cytidine(34)-2'-O)-methyltransferase -
  STRSA0001_RS01300 (STRSA0001_1795) trkA 86149..87498 (+) 1350 WP_002883814.1 Trk system potassium transporter TrkA -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comS comX positive effect
  comX late competence genes positive effect
  comX late competence genes positive effect
  comR comX positive effect
  comR comS positive effect
  comS comS positive effect

Sequence

Protein


Download         Length: 25 a.a.        Molecular weight: 2804.57 Da        Isoelectric Point: 10.1174

>NTDB_id=52 82608..82682(-) (comS) [Streptococcus salivarius SK126]
MKKLKLFTLFSLLITILPYFTGCL*

Nucleotide


Download         Length: 75 bp        

>NTDB_id=52 82608..82682(-) (comS) [Streptococcus salivarius SK126]
TTGAAAAAACTAAAATTATTTACACTATTCTCACTACTTATCACTATCTTGCCCTATTTTACAGGTTGTCTTTAA

XIP

This gene is known to encode the precursor of σX inducing peptide (XIP), which involves in competence development. The mature XIP sequence is displayed as below:

PYFTGCL


Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comS Streptococcus salivarius strain HSISS4

96

100

0.96
  comS Streptococcus thermophilus LMG 18311

79.167

100

0.792
  comS Streptococcus thermophilus LMD-9

79.167

100

0.792

Multiple sequence alignment    



References

[1] Laetitia Fontaine et al. (2010) A novel pheromone quorum-sensing system controls the development of natural competence in Streptococcus thermophilus and Streptococcus salivarius. Journal of Bacteriology 192(5):1444-54. [PMID: 20023010]